Recombinant Mouse Microtubule-Associated Protein 2 (MAP2) Protein (His-SUMO)

Name: Recombinant Mouse Microtubule-Associated Protein 2 (MAP2) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-10868P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Mouse Microtubule-Associated Protein 2 (MAP2) Protein (His-SUMO) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P20357
Target Symbol	MAP2
Synonyms	Map2; Mtap2; Microtubule-associated protein 2; MAP-2
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	GMEGEKLPPVPFAQTFGTNLEDRKQSTEPSIVMPSIGLSAEPPAPKEPKDWFIEMPTESKKDEWGLAAPISPGPLTPMREKDVLEDIPRWEGKQFDSPMPSPFHGGSFTLPLDTMKNERVSEGPRPFAPVFFQSDDKVSLQDPSALATSKESSKDEEPLKDKADKVADVSISEVTTLLGNVHSPVVEGYVGENISGEVKVTTDQEKKETSAPSVQEPTLTETEPQTKLDEKSTVSIEEAVAKKEESFKLRDDKTGVIQTSTEQSFSKEDQKGQEHTIDELKQDSFPISLEQAVTDAAMTSKTLGKVTSEPEAVSERREIQGLFEEKTADKNKLEGAGSATIAEVEMP
Expression Range	213-559aa
Protein Length	Partial
Mol. Weight	54.0kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Subcellular Location	Cytoplasm, cytoskeleton. Cell projection, dendrite.
Database References	KEGG: mmu:17756 STRING: 10090.ENSMUSP00000109650 UniGene: Mm.256966

Gene Functions References

MAP2c levels in different brain regions of aging mice were positively correlated with neuropsin expression. PMID: 24965600
Accumulation of intraneuronal beta-amyloid 42 peptides is associated with early changes in microtubule-associated protein 2 in neurites and synapses. PMID: 23372648
results demonstrate the structural and functional determinant underlying the v-KIND-MAP2 interaction that controls dendrite arborization patterns PMID: 21385318
Increased MAP2 expression in malignant melanoma cells significantly inhibits tumor cell growth and neoplasm invasiveness, and induces apoptosis and cell cycle arrest. PMID: 20100193
Data show that inhibition of ZNF238 (RP58) expression in mouse GNPs decreases the expression of the neuronal differentiation markers MAP2 and NeuN and downregulates the expression of the cell cycle arrest protein p27, a regulator of GNP differentiation. PMID: 20103640
Nitric oxide affects the phosphorylation state of microtubule-associated protein 2 in brain. PMID: 11829530
PKA binding to MAP2 and MAP2 phosphorylation is essential for the selective development of contextual memory. PMID: 12763072
Multiple brain regions exhibit potential for endogenous recovery and/or reorganization of MAP2 after injury of moderate traumatic severity. PMID: 14588114
As young adults, S100B transgenic mice exhibit significantly greater MAP-2-immunoreactivity in the hippocampus, however in older adults, less MAP-2 staining is exhibited. PMID: 15306236
JNK1 phosphorylation of MAP2 plays an important role in defining dendritic architecture in the brain. PMID: 16000625
We also propose MAP2-deficient mice as an ideal system for identifying protein phosphatases essential for dendritic functions. PMID: 16257389
MAP2 was increased in old females compared with both young and middle-age females with no significant difference in males, suggesting it may constitute a compensatory response to age-related functional decline in mice. PMID: 16330151
TTLL7 is a mammalian beta-tubulin polyglutamylase required for growth of MAP2-positive neurites PMID: 16901895
v-KIND mediates a signaling pathway that links Ras and MAP2 to control dendrite growth PMID: 17984326
AKAP150 and Mtap2 put TREK channels at the center of a complex protein network that finely tunes channel trafficking, addressing, and regulation. PMID: 18716213

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.