Recombinant Mouse Lymphotactin Protein (XCL1), Active

Name: Recombinant Mouse Lymphotactin Protein (XCL1), Active
Brand: Beta LifeScience
SKU: BLC-05546P
Availability: InStock

Collections: Buy cytokines, chemokines, and growth factors for research online, Growth factors and receptors for advanced research, High-quality cytokines for advanced research, High-quality recombinant proteins

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Product Overview

Description	Recombinant Mouse Lymphotactin Protein (XCL1), Active is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 97% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human XCR1 transfected murine BaF3 cells is less than 100 ng/ml, corresponding to a specific activity of >1.0x10 4 IU/mg.
Uniprotkb	P47993
Target Symbol	XCL1
Synonyms	Xcl1; Lptn; Ltn; Scyc1; Lymphotactin; C motif chemokine 1; Cytokine SCM-1; Lymphotaxin; Small-inducible cytokine C1
Species	Mus musculus (Mouse)
Expression System	E.Coli
Tag	Tag-Free
Complete Sequence	VGTEVLEESS CVNLQTQRLP VQKIKTYIIW EGAMRAVIFV TKRGLKICAD PEAKWVKAAI KTVDGRASTR KNMAETVPTG AQRSTSTAIT LTG
Expression Range	22-114aa
Protein Length	Full Length of Mature Protein
Mol. Weight	10.1 kDa
Research Area	Immunology
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Chemotactic activity for lymphocytes but not for monocytes or neutrophils. In thymus, mediates medullary accumulation of thymic dendritic cells and contributes to regulatoy T cell development, playing a role in self-tolerance establishment.
Subcellular Location	Secreted.
Protein Families	Intercrine gamma family
Database References	KEGG: mmu:16963 STRING: 10090.ENSMUSP00000027860 UniGene: PMID: 28550205 The interaction between XCL1 and XCR1 plays a crucial role in the classical immunology response. PMID: 27387353 Data show that mice lacking either XCL1 or XCR1 exhibit similar specific defects in intestinal T cell populations as XCR1-dendritic cells (DC)-deficient mice. Therefore it is possible that the XCR1-XCL1 axis itself is directly involved in intestinal T cell homeostasis, via a mechanism that might either involve interaction with XCR1+ DCs or be independent of this pathway. PMID: 27005831 T inflammatory memory CD8 T cells participate to antiviral response and generate secondary memory cells with an advantage in XCL1 production PMID: 22528127 XCL1-mediated accumulation of dendritic cells in the thymic medulla contributes to naturally occurring regulatory T cell development and is regulated by Aire. PMID: 21300913 Data report the identification of a new stable molecular assembly formed between the ATAC and Mediator complexes in mouse embryonic stem cells. PMID: 20508642 The expression patterns of XCR1 and XCL1 were conserved in human and mice blood cells, including certain dendritic cell subsets. PMID: 20541533 MIP-1alpha, MIP-1beta, RANTES, and ATAC/lymphotactin function together with IFN-gamma as type 1 cytokines. PMID: 11972057 Adenovirus-mediated intratumoral Lptn gene transfer potentiates the antibody-targeted superantigen therapy of experimental melanoma. PMID: 12226740 In a brain-targeted HIV-1 Tat transgenic model, Tat expression up-regulates XCL1 production not only in Tat-expressing murine astrocytes, but also in monocytes and macrophages/microglia. PMID: 14734774 Lymphotactin gene-modified dendritoma induces T-cell proliferation and strong cytotoxic T lymphocytes reaction against allogenic hepatocellular carcinoma cells. PMID: 17990361 Results describe the role of XCL1 during the course of the immune response to pulmonary M. tuberculosis infection. PMID: 19097989

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.