Recombinant Mouse Interleukin-1 Receptor-Associated Kinase 3 (IRAK3) Protein (His&Myc)

Name: Recombinant Mouse Interleukin-1 Receptor-Associated Kinase 3 (IRAK3) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-05044P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Interleukin-1 Receptor-Associated Kinase 3 (IRAK3) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q8K4B2
Target Symbol	IRAK3
Synonyms	Irak3Interleukin-1 receptor-associated kinase 3; IRAK-3; EC 2.7.11.1; IL-1 receptor-associated kinase M; IRAK-M
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	MAGRCGARGALSPQLLLFDLPPALLGELCGILDSCDGPLGWRGLAERLSNSWLDVRHIEKYVNQGKSGTRELLWSWAQKNKTIGDLLEVLQDMGHQRAIHLIINYGVSWTPSVQTHHELPFPSFPPEVKHACRENDPGPLEPANVTVDNVLVPEHNEKGTLQKTPISFQSILEGTKHFHKDFLIGEGEIFEVYRVDIRNQAYAVKLFKQEKKMQLKKHWKRFLSELEVLLLFRHPHILELAAYFTETEKLCLVYPYMSNGTLFDRLQCTNGTTPLSWHVRISVLIGIAKAIQYLHNTQPCAVICGNVSSANILLDDQLQPKLTDFAAAHFRPNLEQQSSTINMTGGGRKHLWYMPEEYIRQGRLSVKTDVYSFGIVIMEVLTGCKVVLDDPKHVQLRDLLMELMEKRGLDSCLSFLDRKIPPCPRNFSAKLFSLAGRCVATKAKLRPTMDEVLSSLESTQPSLYFAEDPPTSLKSFRCPSPLFLDNVPSIPVEDDENQNNHSVPPKEVLGTDRVTQKTPFECSQSEVTFLGLDRNRGNRGSEADCNVPSSSHEECWSPELVAPSQDLSPTVISLGSSWEVPGHSYGSKPMEKRCSSGLFCSEHEQSKKQ
Expression Range	1-609aa
Protein Length	Full Length
Mol. Weight	73.5 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Putative inactive protein kinase which regulates signaling downstream of immune receptors including IL1R and Toll-like receptors. Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex. Upon IL33-induced lung inflammation, positively regulates expression of IL6, CSF3, CXCL2 and CCL5 mRNAs in dendritic cells.
Subcellular Location	Cytoplasm. Nucleus.
Protein Families	Protein kinase superfamily, TKL Ser/Thr protein kinase family, Pelle subfamily
Database References	KEGG: mmu:73914 STRING: 10090.ENSMUSP00000020448 UniGene: PMID: 29686383 IRAK3 methylation may be a predictive factor in the transition from colitis to cancer. PMID: 28713897 IRAK-M plays a crucial role in the regulation of allergic airway inflammation by modifying the function of airway epithelia PMID: 28665693 Taken together, these results strongly support a role for IRAK-M in renal injury and identify IRAK-M as a possible modulator in driving an alternatively activated profibrotic macrophage phenotype in unilateral ureteral obstruction-induced chronic kidney disease. PMID: 28701510 polycomb recessive complex 2 repressed the IRAK-M promoter, allowing low levels of expression; following LPS stimulation, the IRAK-M promoter is derepressed, and transcription is induced to allow its expression. PMID: 28011933 this study shows that following Pseudomonas aeruginosa infection, IRAK-M knock-out mice have enhanced lung neutrophilic inflammation and reduced bactertial load PMID: 28120642 Data show that interleukin-1 receptor-associated kinase 3 (IRAK-M) is responsible for regulation of microbial colonization of tumors and STAT3 protein stability in tumor cells, leading to tumor cell proliferation. PMID: 27150039 IRAK-M functions to modulate inflammatory signaling pathways and is critical in maintaining immune system homeostasis in the gut. However, increased IRAK-M is associated with increased disease pathogenesis and increased cancer severity in human patients. PMID: 27939424 IRAK-M may also contribute to myofibroblast conversion. PMID: 26542797 These data demonstrate LTi cells are present in the stomach and promote lymphoid follicle formation in response to infection, but are limited by IRAK-M expression. PMID: 25603827 IL-7 reduced IRAK-M expression and attenuated immune tolerance induced by either LPS or CpGA PMID: 26218271 the results suggest that IRAK-M may be targeted by L. donovani to inhibit TLR-mediated proinflammatory response late during in vitro infection. PMID: 26140693 These data indicate expression of IRAK-M skews lung macrophages toward an alternatively activated profibrotic phenotype, which promotes collagen production, leading to the progression of experimental pulmonary fibrosis. PMID: 25595781 Our study identifies the DAP12/IRAK-M/IL-10 to be a novel molecular pathway in APCs exploited by mycobacterial pathogens, allowing infection a foothold in the lung. PMID: 24172845 This study illustrates how the modulation of innate immune pathways through IRAK-M influences the development of autoimmune diabetes. PMID: 24696448 novel findings provide new insights into the understanding of negative regulatory mechanisms of the TLR4 signaling pathway. PMID: 23872113 Altered gut microbiota promotes colitis-associated cancer in IL-1 receptor-associated kinase M-deficient mice. PMID: 23567778 IRAK-M plays an important role in alcohol-induced liver injury and IRAK-M negatively regulates the innate and possibly the adaptive immune response in the liver reacting to acute insult by alcohol. PMID: 23437317 debris-induced IRAK-M decreases foreign body reactions, but at the same time, the over-expression of IRAK-M may also be detrimental on local intrusion of PAMPs or bacteria PMID: 22941946 IRAK-M mediates TLR7-induced MEKK3-dependent second wave NFjB activation to produce inhibitory molecules PMID: 23376919 IRAK-M impairs host defense during pneumonia caused by a common gram-negative respiratory pathogen. PMID: 22729155 Along with endotoxin, bacterial sonicate is able to induce refractory tolerance in BM-DCs, and IRAK-M plays a role in modulating cell surface expression of MHC class II and CD80 and release of IL-10 during this tolerance. PMID: 22472665 These data suggest that IRAK-M impairs host defense during pneumococcal pneumonia at the primary site of infection at least in part by inhibiting the early immune response. PMID: 22492852 The upregulation of IRAK-M in macrophages is involved in the local immunosuppression around implants, and may contribute to septic and aseptic implant loosening. PMID: 21987497 Endotoxin tolerization in vivo blocked TLR4-driven IRAK4 phosphorylation and activation in macrophages, while increasing expression of IRAK-M, SHIP-1, A20 mRNA, and A20 protein. PMID: 21934070 These data identify a previously unknown function of IRAK-M :suppression of TLR7-mediated autoimmunity-and mutant IRAK-M as a previously unknown genetic risk for murine SLE. PMID: 21875872 Role of IL-1 receptor-associated kinase-M (IRAK-M) in priming of immune and inflammatory responses by nitrogen bisphosphonates. PMID: 21690387 By facilitating IRAK-M expression, DNAX-activation protein (DAP)12 functions to negatively regulate lipopolysaccharide-induced liver dendritic cell maturation. PMID: 21257958 Loss of IRAK-M is associated with colitis. PMID: 20848470 IRAK-M is bound to caspase (CASP)-6 in a complex located near the plasma membrane in resting cells, and the components of this complex redistribute to nucleus and cytoplasm after neutrophil stimulation. PMID: 21098228 findings indicate that systemic sepsis induces epigenetic silencing of cytokine gene expression in lung macrophages, and IRAK-M appears to be a critical mediator of this response PMID: 20585389 manipulation of IRAK-M levels can increase the potency of DC vaccines by enhancing their Ag-presenting function, migration, and longevity PMID: 20817880 these findings suggest that IRAK-M negatively regulates the alternative NFkappaB pathway in a ligand-specific manner. PMID: 19809574 These data suggest that the absence of IRAK-M in the hematopoietic compartment post-bone marrow transplantation enhances pulmonary host defense and mitigates alveolar macrophage sensitivity to the inhibitory effects of PGE(2 PMID: 20439918 IRAK-M is critical to preventing deleterious neutrophil-dependent lung injury during influenza infection of the respiratory tract. PMID: 20042589 Endotoxin tolerance was significantly reduced in macrophages from IRAK-M knockout mice. IRAK-M regulates TLR signaling and innate immune homeostasis. PMID: 12150927 induction of IRAK-M and inhibition of kinase activity of IRAK-1 are crucial to PGN-induced tolerance in macrophages PMID: 14660668 IRAK-M is a key regulator of the bone loss that is due to osteoclastic resorption of bone. PMID: 15809356 IRAK-M induction negatively regulates Toll-like receptor-dependent interleukin-12 p40 production in macrophages in the presence of Mycobacterium tuberculosis lipoarabinomannan PMID: 16263713 These findings indicate that IRAK-M selectively attenuates p38 activation and inhibits innate immunity through stabilizing MKP-1. PMID: 17379480 IRAK-M is directly involved in the regulation of both innate and adaptive immune signaling processes, and deletion of IRAK-M enhances host anti-tumor immune response. PMID: 17477969 reduced expression of IRAK-1 and increased expression of IRAK-M after CpG DNA pretreatment resulted in the hyporesponsiveness of macrophages that leads to the protection of mice from hepatic injury and death caused by CpG DNA/D-GalN. PMID: 18378686 Tolerance to intraluminally administered LPS in the lymphocyte recruitment process was induced by enterobacteria, possibly via the induction of IRAK-M and TGF-beta. PMID: 19225984 The expression of IRAK-M as a negative regulator of TLR7 signaling was markedly augmented in immune tolerant macrophage-like cells while the interleukin-1 receptor-associated kinase (IRAK)-1 functioned normally. PMID: 19251253 IRAK-M is a major mediator of globular adiponectin-induced endotoxin tolerance in primary macrophages PMID: 19414798 TREM-1 activation beneficially impacts pulmonary IRAK-M expression PMID: 19596984

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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