Recombinant Mouse Interferon Regulatory Factor 5 (IRF5) Protein (His-SUMO)

Name: Recombinant Mouse Interferon Regulatory Factor 5 (IRF5) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-10778P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality cytokines for advanced research, High-quality recombinant proteins

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Product Overview

Description	Recombinant Mouse Interferon Regulatory Factor 5 (IRF5) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P56477
Target Symbol	IRF5
Synonyms	Irf5Interferon regulatory factor 5; IRF-5
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	MNHSAPGIPPPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFYIPWRHATRHGPSQDGDNTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFQLFYDGPRDMPPQPYKIYEVCSNGPAPTESQPTDDYVLGEEEEEEEEELQRMLPGLSITEPALPGPPNAPYSLPKEDTKWPPALQPPVGLGPPVPDPNLLAPPSGNPAGFRQLLPEVLEPGPLASSQPPTEPLLPDLLISPHMLPLTDLEIKFQYRGRAPRTLTISNPQGCRLFYSQLEATQEQVELFGPVTLEQVRFPSPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQCKVFWSGPCALAHGSCPNPIQREVKTKLFSLEQFLNELILFQKGQTNTPPPFEIFFCFGEEWPDVKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDLKDHMVEQFKELHHLWQSQQQLQPMVQAPPVAGLDASQGPWPMHPVGMQ
Expression Range	1-497aa
Protein Length	Full Length
Mol. Weight	72.0kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Transcription factor that plays a critical role in innate immunity by activating expression of type I interferon (IFN) IFNA and INFB and inflammatory cytokines downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction downstream of the TLR-activated, MyD88-dependent pathway.
Subcellular Location	Cytoplasm. Nucleus.
Protein Families	IRF family
Database References	KEGG: mmu:27056 STRING: 10090.ENSMUSP00000004392 UniGene: PMID: 28695899 Aged mice expressed higher IRF5 levels in the ischemic brains, suggesting that aging has a significant influence on stroke outcomes in mice, which is probably mediated by age-specific inflammatory responses. PMID: 28905935 this study shows a critical role for IRF5 in regulating allergic airway inflammation PMID: 27759022 IRF5 and IRF5 disease-risk variants increase glycolysis and human m1 macrophage polarization by regulating proximal signaling and Akt2 activation. PMID: 27545875 these results reveal a role for Lyn as a specific suppressor of the TLR-MyD88-IRF5 pathway and illustrate the importance of fine-tuning IRF5 activity for the maintenance of immune homeostasis PMID: 27521268 IRF5 siRNA reverses pancreatitis-induced activation of lung macrophages from M1 phenotype to M2 phenotype in severe acute pancreatitis associated with acute lung injury. PMID: 27895424 Our data show that miR-146b targets IRF5, resulting in the regulation of macrophage activation. Furthermore, miR-146b deficient mice developed intestinal inflammation with enhanced M1 macrophage polarization. PMID: 27825654 data suggest that IRF5 plays a causal role in inflammation, fibrosis and impaired vascular EC function in Tsk/+ mice PMID: 27050551 Collectively, these results indicate a key role for IRF-5 in modulating the host antiviral response in peripheral organs that controls bunyavirus neuroinvasion in mice. PMID: 26468541 studies extend prior ones suggesting that inhibiting IRF5 might be useful for chronic macrophage-induced inflammation and suggest that IRF5 blockade would ameliorate more acute forms of inflammation, including lung injury PMID: 26283380 IRF5 plays an important role in the development of disease in the MRL/lpr mouse model of lupus in the absence of the DOCK2 mutation and that IRF5 is required for the transition from mature B cells to plasma cells. PMID: 25076492 IRF5 expression in microglia is regulated by IRF8. IRF5 directly upregulates P2RX4 expression on microglia in peripheral nerve injury, and may play a role in neuropathic pain. PMID: 24818655 PAR1 suppression of IRF5 and IL-12/23 secretion by macrophages provides a novel mechanism by which the host suppresses the mucosal Th1 and Th17 response to H. pylori infection. PMID: 24866378 This study uncovers a new function for IRF5 in controlling the relative mass of different adipose tissue depots and thus insulin sensitivity in obesity. PMID: 25939064 IKKbeta is an IRF5 kinase that instigates inflammation PMID: 25326420 our results reveal a protective role for IRF5 in lupus-associated atherosclerosis that is mediated through the effects of IRF5 in both immune and nonimmune cells. PMID: 25595782 IRF5 has a critical role in shaping the early innate immune response towards West Nile virus in the draining lymph node, which impacts the spread of virus infection, optimal B cell immunity, and disease pathogenesis. PMID: 25031348 The IRF5 deficiency reduces IFN-alpha, IFN-beta and IL-6 production by Toll-like receptor 9 (TLR9)- and TLR7-stimulated DCs and reduces TLR7- and TLR9-induced IL-6 production by B cells PMID: 23291967 establish the species-invariant role of IRF5 in controlling the inflammatory macrophage phenotype both in vitro and in in vivo PMID: 24453413 These proteins were upregulated in Irf5(-/-) mice. PMID: 23912454 Reduction of IRF5 expression, a factor that regulates macrophage polarization, reduced expression of inflammatory M1 macrophage markers, supported resolution of inflammation, accelerated cutaneous and infarct healing PMID: 24361318 IRF5 levels in Tsk(-/+) hearts were similar to control. PMID: 23251680 Our results establish IRF-3, -5, and -7 as the key transcription factors responsible for mediating the type I IFN and IFN stimulated gene response in myeloid dendritic cells during WNV infection and suggest a novel signaling link between MAVS and IRF-5. PMID: 23300459 Our results show that IRF5 is a crucial driver of lupus development in mice. PMID: 21305501 Irf5-deficient monocytes are defective in their response to peritoneal lavage of pristane-injected mice; pristane-induced Ly6C(hi) antigen-producing monocyte recruitment to the peritoneal cavity is impaired in Irf5-deficient mice. PMID: 22933628 these results identify the IRF-5-Ikaros axis as a critical modulator of IgG2a/c class switching. PMID: 22535200 overexpression of IRF5 in blood cells of systemic lupus erythematosus(SLE) patients may contribute to disease pathogenesis. PMID: 22678902 uncovered a new, IFN-I-independent role of IRF5 in regulating chemokines involved in the homing of pDCs and certain lymphocyte subsets. PMID: 22422888 A spontaneous genomic duplication and frameshift mutation in the guanine exchange factor dedicator of cytokinesis 2 (Dock2) that has arisen in at least a subset of circulating Irf5(-/-) mice and inadvertently been bred to homozygosity. PMID: 22431588 the IRF5/Blimp-1 axis differentially regulates the expression of Nba2 lupus susceptibility genes, and they suggest an important role for the IRF5/Blimp-1/p202 axis in murine lupus susceptibility. PMID: 22116829 The authors show that the myxoma virus-induced innate immune response requires TLR9 and its adaptor MyD88, transcription factors IRF5 and IRF7, and the type I interferon positive-feedback loop mediated by IFNAR1. PMID: 21835795 Levels of Irf5 mRNA and protein were higher in females than in age-matched males. PMID: 20802013 Demonstrate a requirement for IRF5 in the development of SLE via its role in B cells. Antinuclear autoantibodies and Ig glomerular deposits, hallmarks of SLE, are absent in Irf5(-/-) mice challenged to develop SLE by pristane injection. PMID: 20479222 Data show the role of IRF-5 in the B-cell terminal differentiation. PMID: 20176957 IRF5 plays an essential role in lupus pathogenesis in murine models and this is mediated through pathways beyond that of type I IFN production PMID: 20007534 study identifies IRF-5 as a new, principal downstream regulator of the TLR-MyD88 signalling pathway and a potential target of therapeutic intervention to control harmful immune responses PMID: 15665823 we also provide evidence that this TLR synergy is mediated, at least in part, by activation of the transcription factor interferon regulatory factor 5 (IRF5). PMID: 17275788 IRF5 is critical for antiviral immunity and for the induction of apoptosis, but not cell cycle arrest in response to DNA damage, and acts as a tumor suppressor on a pathway that may be distinct from that for p53. PMID: 17360658 IRF5 plays a critical role in interferon (IFN)-alpha and IFN-beta production induced not only by RNA-containing immune complexes but also by conventional Toll-like receptor (TLR)7 and TLR9 ligands. PMID: 17513736 reveal a cell-type-specific function for IRF5 in the complex regulatory mechanism of death-receptor-induced apoptosis PMID: 18268344 reveal the cell type-specific importance of IRF-5 in MyD88-mediated antiviral pathways and the widespread role of IRF-5 in the regulation of inflammatory cytokines PMID: 18332133 Data show that IRF-5, which is activated via the MyD88 pathway, is subjected to TRAF6-mediated K63-linked ubiquitination, which is important for IRF-5 nuclear translocation and target gene regulation. PMID: 18824541 The functional characterization of the novel IRF-5 mutant in transformed B and T cell lines and in ATL and CLL patients may lead to a better understanding of the role of these transcriptional regulators in hematopoietic malignancies. PMID: 19430534

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.