Recombinant Mouse Interferon Regulatory Factor 3 (IRF3) Protein (His&Myc)

Name: Recombinant Mouse Interferon Regulatory Factor 3 (IRF3) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-09314P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Mouse Interferon Regulatory Factor 3 (IRF3) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P70671
Target Symbol	IRF3
Synonyms	Irf3; Interferon regulatory factor 3; IRF-3
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	METPKPRILPWLVSQLDLGQLEGVAWLDESRTRFRIPWKHGLRQDAQMADFGIFQAWAEASGAYTPGKDKPDVSTWKRNFRSALNRKEVLRLAADNSKDPYDPHKVYEFVTPGARDFVHLGASPDTNGKSSLPHSQENLPKLFDGLILGPLKDEGSSDLAIVSDPSQQLPSPNVNNFLNPAPQENPLKQLLAEEQWEFEVTAFYRGRQVFQQTLFCPGGLRLVGSTADMTLPWQPVTLPDPEGFLTDKLVKEYVGQVLKGLGNGLALWQAGQCLWAQRLGHSHAFWALGEELLPDSGRGPDGEVHKDKDGAVFDLRPFVADLIAFMEGSGHSPRYTLWFCMGEMWPQDQPWVKRLVMVKVVPTCLKELLEMAREGGASSLKTVDLHISNSQPISLTSDQYKAYLQDLVEDMDFQATGNI
Expression Range	1-419aa
Protein Length	Full Length
Mol. Weight	51.9 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Subcellular Location	Cytoplasm. Nucleus. Mitochondrion.
Protein Families	IRF family
Database References	KEGG: mmu:54131 STRING: 10090.ENSMUSP00000003284 UniGene: Mm.3960

Gene Functions References

this study shows that activation of IRF3 contributes to IFN-gamma and ISG54 expression during the immune responses to B16F10 tumor growth PMID: 28651122
IRF3 exacerbates P. aeruginosa-induced mortality in mice by inhibiting neutrophil adhesion and recruitment to the lungs PMID: 28181039
IRF3 expression and activation depend on the signal transduction of the the urotensin II/urotensin receptor (UII/UT) system, and play important roles in UII/UT PMID: 27448985
Interruption of IRF3-dependent signaling resulted in decreased cardiac expression of inflammatory cytokines and chemokines and decreased inflammatory cell infiltration of the heart, as well as in attenuated ventricular dilation and improved cardiac function. PMID: 29106401
This study reveals a critical role of NSD3-mediated IRF3 methylation in enhancing antiviral innate immunity. PMID: 29101251
Data show that stimulator of interferon genes (STING)-associated vasculopathy with onset in infancy (SAVI)-associated STING N153S mutation triggers IRF3-independent immune cell dysregulation and lung disease in mice. PMID: 28951494
study provided evidence that the ability of ICP34.5 to control IRF3 activation is through its ability to reverse translational shutoff and sustain the expression of other IFN inhibitors encoded by the virus PMID: 28904192
c-Cbl negatively regulates IFN-beta signaling and cellular antiviral response by promoting IRF3 ubiquitination and degradation. PMID: 27503123
This study did not find non-conservative mutations among SJL, B10.S, B6 and B10 mice in the IRF3 amino acid sequence, and show SJL bone marrow-derived macrophages infected with Theiler's murine encephalomyelitis virus exhibit increased virus RNA replication and infectious virus yields as well as greater IL-6 production than C57Bl strain (including B10.S) cultures. PMID: 28898711
NEMO-IKKbeta Are Essential for IRF3 and NF-kappaB Activation in the cGAS-STING Pathway PMID: 28939760
The IRF-3 pathway is essential for tick-borne encephalitis virus-induced RANTES production in the brain. PMID: 27576490
Yersinia YopJ negatively regulates IRF3-mediated antibacterial response through disruption of STING-mediated cytosolic DNA signaling. PMID: 27742471
IRF3 is a major transcriptional regulator of adipose inflammation and is involved in maintaining systemic glucose and energy homeostasis PMID: 27400129
Findings indicate that STING-TBK1-IRF3 pathway mediates a crosstalk between ER stress and apoptosis during M. bovis infection, which can effectively control intracellular bacteria. PMID: 28018864
GPR146 has an antiviral role in fighting against viral infection, although the GPR146-mediated protection is eliminated by IRF3/HES1-signalling. PMID: 28464285
this study shows that IRF-3-mediated apoptosis of virus-infected cells could be an effective antiviral mechanism, without expression of the interferon-stimulated genes PMID: 27178468
Results establish Irf3, known mostly for its role in antiviral responses, as a transcription factor involved in the induction of Th2 responses through the promotion of pro-Th2 costimulation in CD11b(+) dendritic cells PMID: 27546168
Rubicon specifically interacts with the interferon regulatory factor (IRF) association domain (IAD) of IRF3, and this interaction leads to inhibition of the dimerization of IRF3, which negatively regulates interferon-mediated antiviral response. PMID: 28468885
this study shows that IRF3 in antigen-presenting cells and T cells is required for optimal T-cell effector function and the ability of T cells to influence innate immune function of antigen-presenting cells PMID: 27641636
The mitochondrial damage-cGAS-STING-IRF3 pathway is critically involved in metabolic stress-induced endothelial inflammation. PMID: 28302626
TBK1 complexes required for the phosphorylation of IRF3 and the production of interferon-beta have been identified. PMID: 28159912
this study shows that lipopolysaccharide priming of dendritic cells stabilizes their tolerogenicity through reduction of NF-kappaB and IRF3 signaling PMID: 27859049
this study shows that regulation of the IRF3 pathway can affect multiple cell types and contribute to ameliorate pathogenesis of infection-triggered exacerbation of chronic obstructive pulmonary disease PMID: 28363903
Data show that the formation of a tripartite ribosomal protein S6 kinase 1 (S6K1)-STING membrane protein-TANK-binding kinase 1 (TBK1) complex was necessary for the activation of interferon regulatory factor-3 (IRF3). PMID: 27043414
characterizes SREBP cleavage-activating protein as an essential adaptor in the STING signaling pathway PMID: 26900919
Study identifies crosstalk between PTEN and IRF3 in tumor suppression and innate immunity. PMID: 26692175
results revealed a new paradigm in which the antiviral host factor, IRF3, plays a cell-intrinsic pro-parasitic role. PMID: 25811886
Irf3/IFN activation in hematopoietic stem and progenitor cells expands multipotent progenitors fractions but inhibits HSC mobilization PMID: 25843711
find that IRF3 versus ISGF3 specificity may be critical to limiting IFN-beta production and ISGF3 activation, temporally and spatially, but that partial overlap in their specificity is tolerable and may enhance the effectiveness of the innate-immune response PMID: 25896227
Bipartite nuclear localization signal controls nuclear import and DNA-binding activity of IRF3. PMID: 25994966
PP1 directly interacts with IRF3 and dephosphorylates IRF3 at Ser385 and Ser396, resulting in the suppression of TLR- and RLR-triggered IFN-beta production. PMID: 25239187
our results identify CK2 as a novel regulator of TBK1 and IRF3 and suggest that targeting CK2 by small molecular inhibitor may be a viable approach to prevent and treat viral infections. PMID: 25810395
DNA-PK is a DNA sensor for IRF-3-dependent innate immunity. PMID: 23251783
These data show that IRF3 plays a crucial role in development of Th17 responses and EAE and warrants investigation in human multiple sclerosis. PMID: 25069698
identified IRF3 as a novel regulator of peroxisome proliferator-activated receptor gamma PMID: 24596398
findings demonstrate that IFN-beta, but not IFN-lambda2/3, produced via an IPS-1-IRF3 signaling pathway, is important for hMPV clearance. PMID: 24726644
both IRF3 and IRF4 are induced by adiponectin, with adiponectin-AMPK and adiponectin-PPARalpha signalling regulating IRF3 and IRF4, respectively. PMID: 24627105
These results implicate mitochondria and peroxisomes as new sites for IRF3 regulation by N(pro), and highlight the role of these organelles in the anti-viral pathway. PMID: 24551175
Altogether, IRF3 contributes to early control of Theiler's murine encephalomyelitis virus replication through induction of IL-6 and IFN-beta and support of IL-6 antiviral effects, but contributes to virus-induced hippocampal injury. PMID: 24140628
IRF3 interacts with IKKbeta in the cytoplasm to inhibit IKKbeta/NF-kappaB signaling, thus alleviating hepatic inflammation, insulin resistance, and hepatic steatosis. PMID: 24123166
IRF3 is a key factor controlling melanoma growth through NK-cell activities PMID: 24368188
We describe increased MAIDS resistance in infected IRF-3 knockout and double knockout mice. PMID: 24335302
Optineurin is dispensable for NF-kappaB activation but necessary for optimal IRF3 activation in immune cells. PMID: 24244017
STING-IRF3 pathway links endoplasmic reticulum stress with hepatocyte apoptosis in early alcoholic liver disease. PMID: 24052526
Interferon regulatory factor 3 controls interleukin-17 expression in CD8 T lymphocytes. PMID: 23918362
In summary, the vaccinia virus N2 protein is an intracellular virulence factor that inhibits IRF3 activity in the nucleus. PMID: 23761407
IRF3 may play a key role in innate immune responses against invasion of prion pathogens. PMID: 23093799
IRF-3's transcriptional activity required its coactivators, beta-catenin and CBP, to be modified by HDAC6-mediated deacetylation and protein kinase C isozyme beta (PKC-beta)-mediated phosphorylation, respectively. PMID: 23532979
IRF3 plays a protective role in Aortic Banding-induced hypertrophic response by inactivating ERK1/2 in the heart. PMID: 23307144
Western equine encephalitis virus activated IRF-3-mediated neuronal innate immune pathways in a replication-dependent manner, and abrogation of IRF-3 function enhanced virus-mediated injury. PMID: 23192868

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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