Recombinant Mouse Interferon Gamma / IFNG Protein
Beta LifeScience
SKU/CAT #: BLPSN-2996
Recombinant Mouse Interferon Gamma / IFNG Protein
Beta LifeScience
SKU/CAT #: BLPSN-2996
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | N/A |
Host Species | Mouse |
Accession | NP_032363.1 |
Synonym | Ifg, IFN-g |
Background | IFN gamma, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six alpha-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy |
Description | A DNA sequence encoding the mouse IFNG (NP_032363.1) (Met1-Cys155) was expressed and purified. |
Source | HEK293 |
Predicted N Terminal | His 23 |
AA Sequence | Met1-Cys155 |
Molecular Weight | The recombinant mouse IFNG consists of 133 a.a. and predicts a molecular mass of 15.5 kDa. It migrates as approximately 19.6 and 15.4 kDa band in SDS-PAGE under reducing conditions. |
Purity | (80+7.6)% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | 1. Measured by its binding ability in a functional ELISA.2. Immobilized mouse IFNG at 10 ug/mL (100 uL/well) can bind mouse IFNGR1-Fc. The EC50 of mouse IFNGR1-Fc is 0.04-0.08 ug/mL.3. Measured in antiviral assay using L929 cells infected with vesicular stomatitis virus.The ED50 for this effect is typically 0.5-2 ng/mL. |
Formulation | Lyophilized from sterile PBS, pH 7.4.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |