Recombinant Mouse IL-7RA Protein (C-Fc)

Name: Recombinant Mouse IL-7RA Protein (C-Fc)
Brand: Beta LifeScience
SKU: BL-0119NP
Availability: InStock

BL-0119NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Buy cytokines, chemokines, and growth factors for research online, High-purity interleukins & receptors for research, High-quality recombinant proteins

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Product Overview

Description	Recombinant Mouse Interleukin-7 Receptor Subunit Alpha is produced by our Mammalian expression system and the target gene encoding Glu21-Asp239 is expressed with a human IgG1 Fc tag at the C-terminus.
Accession	P16872
Synonym	Interleukin-7 receptor subunit alpha; Il7r; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CD127
Gene Background	Interleukin 7 Receptor alpha (IL-7Rα), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7Rα is majorly expressed on T cells and their precursors, and early in B cell development as well, prior to the appearance of surface IgM. Dynamic regulation of IL-7Rα is important for the generation of appropriate immune responses.
Molecular Mass	52.1 KDa
Apmol Mass	61-75 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 10% Sucrose, 3% Glycine, 100mM NaCl, 0.1mM EDTA, 0.05% Tween 80, pH 8.0.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Biologically active. Please contact us to obtain bioactivity data.
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
Subcellular Location	Membrane; Single-pass type I membrane protein.
Protein Families	Type I cytokine receptor family, Type 4 subfamily
Database References	KEGG: mmu:16197 STRING: 10090.ENSMUSP00000003981 UniGene: PMID: 30451860 IL-7 receptor (IL-7R) is not strictly required for the development of any innate lymphoid cells subset. PMID: 28361874 the results indicate that endogenous IL-7R signals promote Th1 and Tc1 responses and IFN-gamma- and TNF-alpha production to sustain the persistence of SS-like sialadenitis in NOD mice. These findings suggest that IL-7 and Th1 cytokines could serve as promising therapeutic targets for this prevalent autoimmune disease. PMID: 29680668 this study demonstrates a key role for IL-7R in the generation of microenvironments required for thymic dendritic cells PMID: 28890536 show that the differentiation of group 3 ILCs is controlled by the glutamylation of IL-7Ralpha and the induction of transcription factor Sall3 PMID: 28794449 results demonstrated that forced expression of IL-7R not only improved the functionality of tolerized CD8 T cells, it also acted in synergy with PDL-1 deficiency to further promote CD8 T cell cytotoxicity to self antigens PMID: 29272267 this study shows that short-term blockade of IL-7Ralpha induces detectable changes in co-inhibitory receptor expression and Treg frequencies in peripheral blood of NOD mice PMID: 28356069 results indicate that the induction of IL-7Ralpha expression on dendritic cells (DCs) is critical for thymic stromal lymphopoietin responsiveness and that IL-4 can upregulate IL-7Ralpha on DCs. PMID: 28404633 IL-7RA role in hematopoiesis and development of the lympho-myeloid progenitors in the developing fetal liver PMID: 26235516 These studies provide in vivo evidence that IL-7Ralpha signals positively regulate normal human B-cell production and proliferation beyond the fetal period and suggest that TSLP can replace IL-7 in providing these signals. PMID: 27325567 Bcl6 promoted T follicular helper cell differentiation through antagonizing IL-7R / STAT5a axis. PMID: 26876184 results suggest that LNK suppresses IL-7R/JAK/STAT signaling to restrict pro-/pre-B progenitor expansion and leukemia development, providing a pathogenic mechanism and a potential therapeutic approach for B-ALLs with LNK mutations. PMID: 26974155 The IL-7R plays an important role in the induction of HFD-induced adipogenesis and insulin resistance in mice. PMID: 26152662 IL-7R signaling in regulatory T cells maintains peripheral and allograft tolerance in mice. PMID: 26450881 This work demonstrates that the CNS1 element controls IL-7Ralpha expression and maintenance of peripheral T cells, suggesting differential regulation of IL-7Ralpha expression between central and peripheral lymphoid organs. PMID: 26336149 Inhibition of the IL-7 receptor inhibits tumor growth in murine models of melanoma. PMID: 24619454 The combined loss of Gads and CD127 reveals a novel function of Gads prior to T cell receptor beta expression PMID: 25037454 data suggest that IL-7Ralpha Tyr(449) was essential for IL-7Ralpha signaling in bone marrow B cell development and survival. PMID: 25143441 The development and survival but not function of follicular B cells is dependent on IL-7Ralpha Tyr449 signaling. PMID: 24551160 interleukin-7 receptor mutants induce early T cell precursor acute lymphoblastic leukemia by the block of thymocyte differentiation at the double negative 2 stage at which myeloid lineage and T lymphocyte developmental potential coexist. PMID: 24687960 The transcription factor GATA3 is critical for the development of all IL-7Ralpha-expressing innate lymphoid cells. PMID: 24631153 Expression of insertion mutation in combination with a Notch1 mutant led to the development of much more aggressive T-acute lymphoblastic leukemia than with wild-type IL7Ralpha. PMID: 24174626 These data indicate that IL7R+ B cells have a proinflammatory role in arthritis which can be inhibited by the sympathetic neurotransmitter norepinephrine via inhibition of IL-7R signalling. PMID: 23505234 IL-7Ralpha transgenesis or T-cell-specific ablation of Gfi-1 restored IL-7Ralpha expression and largely ameliorated the development and homeostasis defects of TGF-beta-receptor-deficient T cells. PMID: 23932572 We have analyzed the discrete contributions of the antibody constant (Fc) and IL-7-binding (Fab) domains to the mechanism of binding to IL-7R. PMID: 23610371 IL7Ralpha has a role in potentiating IL-7 bioactivity and in promoting autoimmunity PMID: 23610432 Pre-B cell receptor (BCR) cooperates with IL-7R in expanding the pre-B cell pool, but it also critically controls the differentiation program that shuts off the c-Myc gene in large pre-B cells. PMID: 23420891 IL-7Ralpha differentially controls development and maturation of thymocyte subpopulations in late developmental stages. PMID: 23267098 the in vivo regulatory role of Gfi1 was specific for CD8(+), and not CD4(+) T cells or immature thymocytes. These results identify Gfi1 as a specific transcriptional repressor of the Il7r gene in CD8 T lymphocytes in vivo. PMID: 22865857 show that interleukin-17A (IL-17A)-producing IL-7Ralpha(+) innate lymphoid cells (ILCs) were potent promoters of disease in Tbx21(-/-)Rag2(-/-) ulcerative colitis (TRUC) mice PMID: 23063332 The durable efficacy and the multipronged tolerogenic mechanisms of IL-7Ralpha antibody therapy suggest a unique disease-modifying approach to T1D. PMID: 22733769 Blockade of IL-7/IL-7Ralpha signaling in IL-7-dependent p53(null) cells reduced POT1 expression and caused telomere and chromosome abnormalities similar to those observed in DKO lymphomas. PMID: 22281704 Data suggest that this decrease in IL-7Ralpha is associated with defective hematopoietic development in Down Syndrome. PMID: 21504363 Increased IL-7Ralpha (CD127) expression correlated, as previously reported with increased turnover of CD4 memory cells, however, this was not linked to protection or enhanced response to rechallenge PMID: 22039531 a novel function of Efnb1 and Efnb2 in stabilizing IL-7Ralpha expression at the post-translational level PMID: 22069310 IL-7R-mediated signaling not only maintains the size but also impacts the diversity of the naive T-cell repertoire. PMID: 21339767 IL-7R-dependent survival and differentiation of early T-lineage progenitors is regulated by the BTB/POZ domain transcription factor Miz-1. PMID: 21258009 High expression of IL-7 receptor (IL-R)alpha on colitic CD4-positive memory T cells, but not on other cells, is essential for the development and persistence of chronic colitis. PMID: 21217010 These findings highlight that IL-7Ralpha confers susceptibility by influencing autoimmune Th1/Th17 responses in experimental autoimmune encephalomyelitis model of multiple sclerosis . PMID: 20861865 T-cell homeostasis is maintained through a concerted regulation of Gfi-1-IL-7R-controlled cytokine responsiveness and ERK-mediated TCR signaling strength by the Cdc42-PAK1 signaling axis. PMID: 20937872 increased expression on activated FOXP3+CD4+ regulatory T cells PMID: 20690182 HIV Tat protein colocalizes with CD127 at the cell surface of resting CD8-positive T lymphocytes. PMID: 20660706 Sin1-mTORC2 suppresses rag1,2 and il7r gene expression through Akt2 in B cells. PMID: 20705244 study shows that IL-7Ralpha Y449 is important for lymphocyte transformation PMID: 20440272 Pre-pro-B cell growth-stimulating factor (PPBSF) upregulates IL-7Ralpha chain expression and enables pro-B cells to respond to monomeric IL-7. PMID: 12184921 IL-7R(alpha)(-/-) mice lack detectable numbers of all downstream early B lineage precursors, including pre-pro-B cells PMID: 12208884 IL-7R signals are able to stimulate T cell proliferation in lymphopenic hosts independently of lck-mediated T cell receptor (TCR) signaling, but they can also synergize with TCR signals to facilitate proliferation. PMID: 12244169 IL-2 signaling can diminish IL-7Ralpha expression via a phosphatidylinositol 3-kinase/Akt-dependent mechanism. PMID: 12354940 The inability of B-cell progenitors in the thymus to mature is due to down-regulation of IL7Ralpha through a type 1 IFN receptor-independent mechanism PMID: 12393710 Bax deficiency partially corrects interleukin-7 receptor alpha deficiency PMID: 12433363

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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