Recombinant Mouse IL-3 Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0032
Recombinant Mouse IL-3 (2 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 15 kDa and purity greater than 95%.
Recombinant Mouse IL-3 (2 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 15 kDa and purity greater than 95%.

Recombinant Mouse IL-3 Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0032
/
Size

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Product Overview

Product Name Recombinant Mouse IL-3 Protein
Target Symbol IL3
Alternative Names Interleukin 3, IL3, Mast Cell Growth Factor, Multilineage Colony Stimulating Factor, Multipotential Colony Stimulating Factor, Hematopoietic Growth Factor, P-Cell Stimulating Factor, MULTI-CSF, MCGF, MGC79398, MGC79399
Accession Number P01586
Expression System E. coli.
Molecular Weight 15.2 kDa (monomer, predicted)
Sequence Asp33-Cys166, with an N-terminal Met
Purity >95% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining.
Activity Determined by a cell proliferation assay using mouse NFS-60 cells. The ED50 for this effect is typically <0.1 ng/mL.
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level <0.5 EU per 1 ug of the protein as determined by the LAL method.
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS.
Target Function Interleukin-3 (IL-3) is a pleiotropic cytokine that plays a crucial role in the proliferation, differentiation, and survival of hematopoietic stem cells and myeloid progenitor cells. In particular, IL-3 regulates the development of basophils, dendritic cells, eosinophils, erythrocytes, macrophages, mast cells, megakaryocytes, monocytes, and neutrophils. IL-3 is produced by a variety of cells, including activated T cells, B cells, mast cells, and vascular endothelial cells. Signaling takes place through binding to IL-3R alpha (CD123) that heterodimerizes with the common beta chain (CD131).
Tissue Specificity IL-3 expression has been identified in bone marrow stromal cells and vascular endothelial tissue.
Cellular Localization Secreted protein
Involvement In Disease Dysregulation of IL-3 is associated with asthma, allergic rhinitis, atopic dermatitis, acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), aplastic anemia, multiple sclerosis (MS), rheumatoid arthritis, systemic lupus erythematosus (SLE), inflammatory bowel disease (IBD), psoriasis, rheumatoid arthritis, and myelodysplastic syndromes (MDS).

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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