Recombinant Mouse IDO Protein (N-6His)

Name: Recombinant Mouse IDO Protein (N-6His)
Brand: Beta LifeScience
SKU: BL-1632NP
Availability: InStock

BL-1632NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Featured immune checkpoint protein molecules, High-quality recombinant proteins, Immune checkpoint proteins

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Product Overview

Description	Recombinant Mouse Indoleamine 2,3-dioxygenase is produced by our E.coli expression system and the target gene encoding Met1-Pro407 is expressed with a 6His tag at the N-terminus.
Accession	P28776
Synonym	Indole 2;3-dioxygenase; Indoleamine 2;3-dioxygenase 1; IDO-1; IDO1; IDO; INDO
Gene Background	Indoleamine 2,3-dioxygenase (IDO) is a heme enzyme that initiates the oxidative degradation of the least abundant, essential amino acid, l-tryptophan, along the kynurenine pathway. This protein is normally expressed in the dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. IDO activity is associated with immunosuppression and immune attenuation. Several studies showed that IDO can contribute to immune escape when expressed directly in tumor cells or when expressed in immunosuppressive antigen presenting cells such as tolerogenic dendritic cells or tumor associated macrophages. IDO also is a promising therapeutic target for the treatment of cancer, chronic viral infections, and other diseases characterized by pathological immune suppression.
Molecular Mass	47.1 KDa
Apmol Mass	40-50 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 0.01% Tween 80, 1mM EDTA, 50% Glycerol,pH 8.0.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity. Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection (Ref. 3).
Subcellular Location	Cytoplasm, cytosol.
Protein Families	Indoleamine 2,3-dioxygenase family
Database References	KEGG: mmu:15930 STRING: 10090.ENSMUSP00000033956 UniGene: PMID: 28402179 Study showed that the knockout of IDO prevented vascular smooth muscle cells apoptosis in AngII -treated Ldlr-/- mice fed with HFD, suggesting a detrimental role of IDO in abdominal aortic aneurysm formation. PMID: 29494675 The KYNurenine pathway of IDO1-mediated Tryptophan metabolism plays a critical role in depressive symptoms associated with IFN-alpha therapy. PMID: 27436416 IDO is a critical regulator of acute pulmonary inflammation . PMID: 28673995 Data suggest that Indoleamine 2,3-dioxygenase 1 (IDO1) appears to be a potential hallmark of liver lesions, and its deficiency protects mice from CCl4-induced fibrosis mediated by Th17 cells down-regulation and tryptophan 2,3-dioxygenase (TDO) compensatory increase. PMID: 28465467 Indoleamine 2,3-dioxygenase regulates anti-tumor immunity in lung cancer by metabolic reprogramming of immune cells in the tumor microenvironment PMID: 27705910 Findings suggest non-redundant neurophysiological roles for indoleamine 2,3-dioxygenase 1, indoleamine 2,3-dioxygenase 2 and tryptophan 2,3-dioxygenase in modulating brain activities and metabolism. PMID: 27316339 These results show IDO is upregulated with RSV infection and this upregulation likely participates with IFN-gamma in inhibition of virus replication and suppression of some host cell responses to infection. PMID: 28963880 Lipopolysaccharide (LPS) stimulation increased the expression and activity of the immunoregulatory enzyme IDO1 in hepatic stellate cells (HSCs), and LPS/HSCs stimulated aryl hydrocarbon receptor (AhR) signaling in cocultured regulatory T cells. PMID: 27581538 this study shows that the presence of IFN-alpha at antigen sensitization activates an IDO1/TGF-beta-dependent anti-inflammatory program that upon antigenic rechallenge prevents inflammation via plasmacytoid dendritic cells PMID: 27647832 The deficiency of indoleamine 2,3-dioxygenase aggravates the carbon tetrachloride-induced liver fibrosis in mice. PMID: 27598994 Across strains, networks depicted a predominance of genes under-expressed in microglia relative to macrophages that may be a precursor for the different response of both cell types to challenges. The detected transcriptome differences enhance the understanding of the role of IDO1 in the microglia transcriptome under unchallenged conditions. PMID: 27314674 Data show that indoleamine 23-dioxygenase 1 (IDO-1) inhibitors 1-methyl-D-tryptophan was able to alleviate most of the behavioural changes resulting from unpredictable chronic mild stress (UCMS) exposure. PMID: 27828964 IDO did not play a pivotal role in the suppression of allergic airway inflammation through adipose-derived stem cells, suggesting that it is not the major regulator responsible for suppressing allergic airway inflammation. PMID: 27812173 Aortic Plasmacytoid dendritic cells expressed CCR9 and indoleamine 2,3-dioxygenase 1 (IDO-1), an enzyme involved in driving the generation of regulatory T cells (Tregs). PMID: 27166946 Indoleamine-2,3-dioxygenase (IDO) production by Plasmacytoid dendritic cells (pDCs)is necessary to confer suppressive function to T-Cells, Regulatory (Tregs) in experimental autoimmune encephalomyelitis (EAE). PMID: 27470005 our findings support the hypothesis elevated IDO activity in non-CNS due to virus infections causes pain hypersensitivity PMID: 27168185 this study shows that IDO overexpression in dendritic cells attenuates acute allograft rejection PMID: 27107370 This insight into IDO1's involvement in pro-tumorigenic inflammatory neovascularization may have important ramifications for IDO1 inhibitor development, not only in cancer where clinical trials are currently ongoing, but in other disease indications associated with neovascularization as well. PMID: 27889479 Inhibition of IDO activity ameliorated Japanese encephalitis via enhancement of antiviral IFN-I/II innate and adaptive T-cell responses and increased central nervous system infiltration of peripheral leukocytes. PMID: 27090635 Results suggest that IDO expression is implicated in immunosuppression and tumor progression in glioma cells; combining IDO inhibition with standard TMZ treatment could be an encouraging therapeutic strategy for patients with malignant glioma PMID: 26636389 Data show that the expression of indoleamine 2, 3-dioxygenase 1 (IDO) was decreased after tumor cells were infected with Salmonella. PMID: 26517244 Severity of sodium dodecyl sulfate-induced colitis is reduced in Ido1-deficient mice with down-regulation of TLR-MyD88-NF-kB transcriptional networks. PMID: 26610689 Data implicate indoleamine 2,3-dioxygenase-dependent neurotoxic kynurenine metabolism as a pathogenic factor for cognitive dysfunction in inflammation-induced depressive disorders and a potential novel target for the treatment of these disorders. PMID: 26130057 IDO1 deficiency does not affect inflammation in Systemic Juvenile Idiopathic Arthritis, Secondary Hemophagocytic Lymphohistiocytosis and a T cell-triggered cytokine release model. PMID: 26914138 Increased expression of IDO in liver cell adenomas compared to the surrounding normal tissue may create a microenvironment that promotes the progression of HCC by suppressing the proliferation of cytotoxic T lymphocytes and enhancing Tregs. PMID: 26727596 Chimeric vaccine stimulation of human dendritic cell IDO1 occurs via the non-canonical NF-kappaB pathway. PMID: 26881431 Absence of Ido1 protects against atherosclerosis through increase of Il10. PMID: 26235422 Indoleamine 2,3-Dioxygenase Is Involved in the Inflammation Response of Corneal Epithelial Cells to Aspergillus fumigatus Infections PMID: 26361229 the role of IFN-lambda in IDO regulation was investigated after influenza infection of respiratory epithelial cells. PMID: 25756191 TNF-alpha mediates stress-induced depression by upregulating indoleamine 2,3-dioxygenase in a mouse model of unpredictable chronic mild stress. PMID: 26083579 Experimental hemophilic mouse models with or without functional IDO1 revealed that tryptophan metabolites, which result from IDO1 activity, prevent generation of anti-FVIII antibodies. PMID: 26426076 Data indicate indoleamine 23-dioxygenase 1 IDO1 induction in B cells as a negative regulatory mechanism of the T Cell-independent antigens (TI) humoral immune response. PMID: 26216892 IFN-gamma coordinately induces IDO1 and a tryptophan-selective transporter in human colonic epithelial cells and mouse dendritic cells with a positive feedback mechanism via kynurenine-AhR signaling. PMID: 25450809 The data showed that there is not significant effect of IDO1 or TDO2 on mortality in pneumococcal meningitis. PMID: 24844751 dendritic cell-based immune response mediated by interferon-gamma-induced IDO expression via GSK-3beta activity not only regulates CD8(+) T-cell proliferation and cytotoxic T lymphocyte activity but also modulates OVA-pulsed DC vaccination against EG7 thymoma PMID: 25814664 Data suggest that transferred TGF-beta-induced regulatory T cells (iTregs) could induce tolerogenic splenic dendritic cells and these cells could effectively dampen collagen-induced arthritis in an indoleamine 2,3-dioxygenase (IDO)-dependent manner. PMID: 25405209 20 weeks of Western diet altered LPS-induced depressive-like behavior compared to LPS-treated lean mice and exacerbated hippocampal and hypothalamic proinflammatory cytokine expression and brain IDO activation. PMID: 24681251 B7-2 costimulation and intracellular indoleamine 2,3-dioxygenase expression is reduced in umbilical cord blood as compared to adult peripheral blood. PMID: 24930629 These data indicate that activation of brain IDO1 is sufficient to induce depression-like behaviors of mice in response to central LPS. PMID: 23866724 IDO2 is critical for IDO1-mediated T-cell regulation and exerts a non-redundant function in inflammation. PMID: 24402311 Inhibitors were used to clarify the role of IDO in graft-vs-tumor reactions after allogeneic stem cell transplantation plus donor leukocyte infusion. IDO1 expression in tumor tissues and TDLN, but not spleen, was increased in the mice receiving DLI. PMID: 24971697 The role of IDO during initial host response to influenza infection was studied by using a specific inhibitor. IDO inhibition enhanced lung proinflammatory cytokine gene and protein expression at 24 and 48 h post influenza virus infection. PMID: 24799604 engagement of CD80/86 by CTLA-4 induced activation of the enzyme indoleamine 2,3-dioxygenase (IDO) in osteoclast precursors, which degraded tryptophan and promoted apoptosis PMID: 24807557 The study indicates that IDO1 is spatiotemporally expressed in activated microglia during acute viral encephalitis by encephalomyocarditis virus. PMID: 24530381 Systemic primary and recall T-cell CD8 responses to viral antigens are controlled by IDO. PMID: 24587363 tumor-derived IDO promotes the peritoneal dissemination of ovarian cancer through creating an immunotolerogenic environment within the peritoneal cavity PMID: 24826982 Induction of hepatitis B virus surface antigen-specific cytotoxic T lymphocytes can be up-regulated by the inhibition of indoleamine 2, 3-dioxygenase activity. PMID: 24580128 Data indicate that the frequency and absolute number of Treg cells increased in indoleamine 2,3-dioxygenase (IDO) expressing fibroblast environment PMID: 23891282 we address this issue with the development of IDO1 monoclonal antibody 4B7 which specifically recognizes the murine enzyme in tissue sections, offering a reliable tool for immunohistology in preclinical disease models. PMID: 24123235

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