Recombinant Mouse Guanine nucleotide-binding protein G (GNAQ) Protein (His&Myc)

Name: Recombinant Mouse Guanine nucleotide-binding protein G (GNAQ) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-02888P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Mouse Guanine nucleotide-binding protein G (GNAQ) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P21279
Target Symbol	GNAQ
Synonyms	GnaqGuanine nucleotide-binding protein G(q) subunit alpha; Guanine nucleotide-binding protein alpha-q
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	MTLESIMACCLSEEAKEARRINDEIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFENPYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPSYLPTQQDVLRVRVPTTGIIEYPFDLQSVIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQAAREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV
Expression Range	1-359aa
Protein Length	Full Length
Mol. Weight	49.2 kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity. Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs).
Subcellular Location	Cell membrane; Lipid-anchor. Golgi apparatus. Nucleus. Nucleus membrane.
Protein Families	G-alpha family, G(q) subfamily
Database References	KEGG: mmu:14682 STRING: 10090.ENSMUSP00000025541 UniGene: PMID: 29223637 these data highlighted the critical role of Galphaq in regulating Th17 differentiation and multiple sclerosis pathogenesis PMID: 28216651 Emergence of a malignant phenotype may therefore arise from both under- and overexpression of Galpha11/q signaling, implicating its upstream regulation as a potential therapeutic target in a host of pathologic conditions PMID: 28301547 Galphaq regulates the development of rheumatoid arthritis by modulating Th1 differentiation PMID: 28197018 Fluid shear stress acts on the Galphaq-ERK5 signaling pathway to upregulate Cyclin B1 and CDK1 expression, thereby resulting in MC3T3-E1 cell proliferation. PMID: 27115838 cell-penetrating peptides should effectively inhibit active Galphaq in cells and that these and genetically encoded sequences may find application as molecular probes, drug leads, and biosensors to monitor the spatiotemporal activation of Galphaq in cells. PMID: 27742837 The Galphas and Galphaq peptides adopt different orientations in beta2-AR and V1AR, respectively. The beta2-AR/Galphas peptide interface is dominated by electrostatic interactions, whereas the V1AR/Galphaq peptide interactions are predominantly hydrophobic. PMID: 27330078 The betaARKrgs peptide, but not endogenous GRK2, interacted with Galpha(q) and interfered with signaling through this G protein. These data support the development of GRK2-based therapeutic approaches to prevent hypertrophy and heart failure. PMID: 27016525 Galphaq/Galpha11 signaling pathways play a pivotal role in gene activity patterns during cardiac remodeling. PMID: 26476043 Developmental AHR activation by pollutants, and other exogenous ligands, increases the likelihood that Gnaq knockout mice will develop autoimmune diseases later in life. PMID: 26363170 demonstrate that the ability of an MC4R agonist delivered to PVN to inhibit food intake is lost in mice lacking G(q/11)alpha in the paraventricular nucleus of the hypothalamus but not in animals deficient for G(s)alpha PMID: 26595811 In studies with Gnaq(-/-) mice, Galphaq inhibited the differentiation of Th17 cell via regulating the activity of ERK-1/2 to control the expression of STAT3 and RORalpha. PMID: 25732870 results define specific modulation of spatial working memory and psychostimulant responses through disruptions in G(alphaq) signaling within cerebral cortical glutamatergic neurons PMID: 25963901 Gnaq was selectively inactivated in GnRH neurons of global Gna11--null mice. Galphaq/11-coupled signaling is a major conduit of GnRH secretion, results with these mice showed a role for non-Galphaq/11-coupled signaling in reproductive development and function. PMID: 26377475 Data show that the expression of the guanine nucleotide binding protein alpha q GNAQ(Q209L) allele induced causes the rapid development of uveal melanoma, with local invasion of blood vessels and multiple tumors developing in the lungs. PMID: 26113083 Fluorosed mouse ameloblasts have increased SATB1 retention and Galphaq activity. PMID: 25090413 Gq activation stimulated calcineurin activity, resulting in CN-dependent upregulation of TRPC6 in murine kidneys. PMID: 25844902 activation of ERK5 MAPK by model Gq-coupled GPCRs does not depend on receptor internalization, beta-arrestin recruitment or receptor phosphorylation but rather is dependent on Galphaq-signalling. PMID: 24358341 Precise temporal control of Gq signals in 5-HT2c-R domains in GABAergic neurons upstream of 5-HT neurons provides negative feedback regulation of serotonergic firing to modulate anxiety-like behavior in mice. PMID: 24733892 Lack of TRPC4 potentiation in neurons in the lateral nucleus of the amygdala affects two Galphaq/11 protein-coupled signaling pathways, implicated in fear-related behavior. PMID: 24599464 Galpha11 is essential for the development of cardiac hypertrophy in type I-diabetes. PMID: 22560942 This is the first conclusive evidence for the physiological importance of the activation of Gq/11 by the LH receptor and for the involvement of Galphaq/11 in ovulation. PMID: 23836924 Inhibition of Galphaq alters cell proliferation and is associated with decreased MMP-9 expression and activity PMID: 22595017 Suggest the involvement of the Gnaq and phosphatidylinositol 3-kinase signaling cascades in mediating melatonin effects in pancreatic alpha-cells. PMID: 22672634 Src family kinases, PI3 kinase and protein kinase C synergize to mediate Gq-dependent platelet activation PMID: 23066026 PKCzeta is essential for Gq-dependent ERK5 activation in cardiomyocytes and cardiac fibroblasts and indicate a key cardiac physiological role for the Galpha(q)/PKCzeta/ERK5 signaling axis. PMID: 22232556 Galpha(q) blockade abrogated the development of the heart failure phenotype in tetracycline-transactivating factor/V1A-transgenic mice. PMID: 21747049 rat monoclonal antibodies, specifically recognizing the deamidated Galpha(q), to detect the actions of Pasteurella multocida toxin deamidated Galpha(q) only under reducing conditions. PMID: 21624053 the Galpha(q) signal plays an inhibitory role in the PTH osteoanabolic action, suggesting that its suppression may lead to a novel treatment in combination with PTH against osteoporosis. PMID: 21345793 study describe the structure of PLC-beta3 in an activated complex with Galphaq, which together with supporting biochemical and physiological analyses reveals its mechanism of transmembrane signaling PMID: 20966218 Cellular G(i)-G(q) synergism derives from direct supra-additive stimulation of phospholipase C-beta3 by G protein subunits Gbetagamma and Galpha(q). PMID: 20579885 Gnaq expression by B cells is necessary for normal peripheral B cell development and tolerance induction and also plays a role in preventing early onset mortality in mice. PMID: 20624888 In Galphaq-induced cardiomyopathy, myocyte contractile dysfunction is mediated, at least in part, by 1 or more oxidative posttranslational modifications of SERCA. PMID: 20508180 Both PKCzeta and MEK5 associate to G alpha(q) upon activation of GPCR, thus forming a ternary complex that seems essential for the activation of ERK5. PMID: 20200162 In mouse pacreatic acini Galphaq links cholecystokinin stimulation to activation of Rac1, actin cytoskeletal reorganization and amylase secretion. PMID: 19940064 Ca(2+)-mediated inhibition of AC5/6 is an important mechanism of purinergic receptor-induced decline of cAMP in vascular smooth muscle cells. PMID: 19889965 MEKK1 is essential for cardiac hypertrophy and dysfunction induced by Gq PMID: 11891332 Activation of Gq-coupled receptors on resident leukocytes in the lung elicits expression of GM-CSF, which in turn is required for allergen-induced pulmonary eosinophilia, identifying a novel pathway to pulmonary pathology in diseases such as asthma. PMID: 11907117 pathway is involved in orphan G protein-coupled receptors MrgA1 and MrgC11 which are activated by RF-amide-related peptides PMID: 12397184 The vesicular monoamine content regulates VMAT2 activity through Galphaq in mouse platelets. PMID: 12604601 cholinergic receptor-mediated responses are dependent on Galphaq-mediated signaling events and Galphaq is a potential target of preventative/intervening therapies for lung dysfunction. PMID: 12611815 antisense to Galpha(q/11) significantly inhibited IL-6 production compared to control PMID: 12829380 an Akt-mediated cell survival pathway is compromised by the diminished availability of PIP2 elicited by pathological levels of Gq activity in cardiomyocytes PMID: 12900409 These data suggest that slow myosin heavy chain 2 repression in innervated fast pectoralis major fibers is mediated by cell signaling involving muscarinic acetylcholine receptors, G(alpha)q, and protein kinase C. PMID: 12952937 RGS proteins block Galpha(q)-mediated signal production by competing with downstream effectors for Galpha(q) binding. PMID: 14630933 We identified three of four such mutations as hypermorphic alleles of Gnaq and Gna11, which encode widely expressed Galphaq subunits, act in an additive and quantitative manner, and require Ednrb. PMID: 15322542 PC1 signaling elevates intracellular Ca(2+), activates Galpha(q) and PLC, which then activates calcineurin and NFAT PMID: 15466861 prolonged activation of the Gq pathway desensitizes GnRH-induced signaling by selectively down-regulating the PLC-PKC-Ca2+ pathway, leading to reduced LHbeta synthesis and LH secretion PMID: 15878957 the inhibitory effect of Galpha q on the cardiac LTCC is mediated by inhibition of PI3K PMID: 16186103 activation of Galpha q in cardiac myocytes of adult mice causes a dilated cardiomyopathy that requires the activation of PLCbeta PMID: 16210321

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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Recombinant Mouse Guanine nucleotide-binding protein G (GNAQ) Protein (His&Myc)

Recombinant Mouse Guanine nucleotide-binding protein G (GNAQ) Protein (His&Myc)

Product Overview

Target Details

FAQs