Recombinant Mouse Guanine nucleotide-binding protein G (GNAO1) Protein (His)

Name: Recombinant Mouse Guanine nucleotide-binding protein G (GNAO1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-09744P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Mouse Guanine nucleotide-binding protein G (GNAO1) Protein (His) is produced by our Yeast expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P18872
Target Symbol	GNAO1
Synonyms	Gnao1; Gna0; GnaoGuanine nucleotide-binding protein G(o) subunit alpha
Species	Mus musculus (Mouse)
Expression System	Yeast
Tag	N-6His
Target Protein Sequence	GCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESGKSTIVKQMKIIHEDGFSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGVEYGDKERKTDSKMVCDVVSRMEDTEPFSAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAGDYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVALSGYDQVLHEDETTNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPLTICFPEYPGSNTYEDAAAYIQTQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVTDIIIANNLRGCGLY
Expression Range	2-354aa
Protein Length	Full Length of Mature Protein
Mol. Weight	42.0kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Stimulated by RGS14. The G(o) protein function is not clear.
Subcellular Location	Cell membrane. Membrane; Lipid-anchor.
Protein Families	G-alpha family, G(i/o/t/z) subfamily
Database References	KEGG: mmu:14681 STRING: 10090.ENSMUSP00000034198 UniGene: Mm.251445

Gene Functions References

Galphao +/- mice developed tolerance faster and displayed more severe naltrexone-precipitated withdrawal in some behaviors than did wild-type PMID: 28971229
Go signaling is essential for the structural and functional integrity of the main olfactory epithelium and for specification of olfactory bulb interneurons. PMID: 27625425
Data obtained support the hypothesis that GalphaO-mediated signaling within the presympathetic circuits of the rostral ventral lateral medulla contributes to the autonomic control of the heart. PMID: 27528004
Necdin is a candidate downstream effector for Galphao. PMID: 25012566
This heterozygous Gnao1 +/G184S mutation produces early neonatal and adult lethality accompanied by epileptiform discharges. PMID: 24700286
These results highlight that sensory neurons of the Galphao-expressing vomeronasal subsystem, together with the receptors they express and the molecular cues they detect, control a wide range of fundamental mating and reproductive behaviors in female mice. PMID: 24886577
The Goalpha expressing vomeronasal receptor cells led to all areas of the accessory olfactory bulb (AOB). PMID: 22383629
Data indicate that rectifying potassium channel Kir2.4 interacts with G-protein subunit Galphao. PMID: 23339194
Data show that Galphao is an essential requirement for the display of male-male territorial aggression as well as maternal aggression. PMID: 21768373
Data suggest that the novel R234H mutation imparts oncogenic properties to Galphao by accelerating nucleotide exchange and rendering it constitutively active. PMID: 21317923
The 2.9 A crystal structure of the enigmatic, neuronal G protein Galpha(o) in the GTP hydrolytic transition state, complexed with RGS16. PMID: 18434540
Go alpha subunit mainly appears to be bound to the cell membrane and concentrated in the thin finger-like dendrites of rod bipolar cells, as well as in green fluorescent protein-positive cone bipolar cells. PMID: 12454992
Binding of activated Galphao to GRIN1 induces activation of Cdc42, which leads to morphological changes in neuronal cells. PMID: 15585744
our findings establish an essential role for G(o) in the antiadrenergic effect of muscarinic agent on hyperpolarization current PMID: 18156202
Go2alpha optimizes vesicular filling which is instrumental for normal dopamine functioning and for the development of drug-induced behavioral sensitization. PMID: 18606864
Data show that Galphao colocalized with GRIN1 at neuronal dendrites and axons, and Galphao-GRIN1 pathway could mediate roles in neuronal migration and differentiation at embryonic stages. PMID: 18729205
Report role of Galpha0 in the control of in vivo heart rate dynamics. PMID: 18832081
Regulator of G protein signaling protein suppression of Galphao protein-mediated alpha2A adrenergic receptor inhibition of mouse hippocampal CA3 epileptiform activity. PMID: 19225179
The G alpha(o) protein in the locus coeruleus as contributing to interindividual variability in physical dependence on opioids in mice. PMID: 19460419
Data indicate that MC4R mutant is able to selectively interact with G(i/o) proteins. PMID: 19648111

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.