Recombinant Mouse Guanine nucleotide-binding protein G (GNAI2) Protein (His)

Name: Recombinant Mouse Guanine nucleotide-binding protein G (GNAI2) Protein (His)
Brand: Beta LifeScience
SKU: BLC-01932P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins, Recombinant proteins fall special offers - full-length proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Description	Recombinant Mouse Guanine nucleotide-binding protein G (GNAI2) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P08752
Target Symbol	GNAI2
Synonyms	Adenylate cyclase-inhibiting G alpha protein
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	GCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPQRADDARQLFALSCAAEEQGMLPEDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITQSSLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF
Expression Range	2-355aa
Protein Length	Full Length of Mature Protein
Mol. Weight	44.4 kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.
Subcellular Location	Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell membrane. Membrane; Lipid-anchor.
Protein Families	G-alpha family, G(i/o/t/z) subfamily
Database References	KEGG: mmu:14678 STRING: 10090.ENSMUSP00000057543 UniGene: PMID: 27499046 This study reveals how RGS proteins modulate Galphai2 signaling to facilitate thymocyte egress and T cell trafficking. PMID: 28235863 this study shows that Galphai2 controls arrest in response to chemokine stimulation of neutrophils PMID: 26976957 In Galphai2-knockdown mice, they showed defects in social interaction, novelty recognition and active avoidance learning. PMID: 27787898 Data show that inhibitory (Galphai2) and stimulatory (GalphasL) G-protein subunits produced minor atrophic and hypertrophic changes in muscle mass, and Galphai2 over-expression prevented AAV:beta2-adrenoceptor (beta2-AR) mediated hypertrophy. PMID: 26972746 RGS proteins and Galphai2 play a significant role in regulating states of wakefulness, NREM sleep, and REM sleep PMID: 26564126 Kinocilium is essential for proper localization of Lgn, as well as Gai and aPKC, suggesting that cilium function plays a role in positioning of apical proteins critical for hearing. PMID: 26662512 Galphai2 deficiency combined with cardiac beta1-adrenoceptor overexpression strongly impaired survival and cardiac function, leading to dilated cardiomyopathy. PMID: 26464333 An essential role for Galpha(i2) in Smoothened-stimulated epithelial cell proliferation in the mammary gland. PMID: 26373672 Platelet Galphai2 not only controls hemostatic and thrombotic responses but also is critical for the development of ischemia/reperfusion injury in vivo. PMID: 25944935 Data show that disrupting resistance to inhibitors of cholinesterase 8A (Ric-8A) expression in hematopoietic cells results in a loss of GTP-binding protein alpha subunits Galphai2, Galphai3, and Galphaq. PMID: 26232433 Myocardial ischemia promptly regulated cardiac mRNA and with a slight delay protein levels of both Galphai2 and Galphai3, indicating important roles for both Galphai isoforms. PMID: 24858945 RGS protein/Galphai2 interactions have an essential role in B lymphocyte-directed cell migration and trafficking. PMID: 25617475 Data (including data from knockout mice) suggest that Gnai2 in islet beta-cells is necessary for up-regulation of insulin secretion in response to high glucose in presence of L-arginine or L-ornithine. PMID: 25205820 The absence of Galphai2 and Galphai3 in B cells profoundly disturbs the architecture of lymphoid organs with loss of B cell compartments in the spleen, thymus, lymph nodes, and gastrointestinal tract. PMID: 23977324 Galphai2(Q205L) regulates satellite cell differentiation into myotubes in a protein kinase C - and histone deacetylase -dependent manner PMID: 24298018 Galphas- and Galphai2-mediated signaling in the sinoatrial node is important in the reciprocal regulation of heart rate through the autonomic nervous system. PMID: 23697798 Galphai2-deficient mice spontaneously develop colitis and colonic neoplasms the severity of which are determined by the interplay between Th1 and Th2 cell pathways. PMID: 22962436 Go2alpha protein subunit regulates axonal growth and branching and may increase Rap1GAP activity, thereby effecting the Rap1 activation/deactivation cycle. PMID: 23373526 Galphai2 is required for endothelial neutrophil transmigration but not neutrophil chemotaxis and adhesion. PMID: 23225882 These studies support our hypothesis that G-alpha(i2) proteins are novel negative regulators of Toll-like receptor activation. PMID: 22581266 RGS proteins establish a threshold for Galpha(i2) activation, helping to coordinate desensitization mechanisms during inflammation. PMID: 22966200 A quantitative study of the mechanisms behind thymic atrophy in Galphai2-deficient mice during colitis development. PMID: 22590596 Galphai2 is an essential Galphai protein required for inflammation-induced migration of monocytes and macrophages in Galphai2- or Galphai3-deficient mice. PMID: 22706085 analysis of Galphai2- and Galphai3-specific regulation of voltage-dependent L-type calcium channels in cardiomyocytes PMID: 21966394 Demonstrate that signalling through Gai2 protects the heart from ischaemic injury and that this process is modulated by endogenous RGS proteins. PMID: 21349876 Cecal ligation and puncture -induced plasma TNFalpha, IL-6, bacterial load in peritoneal fluid, blood and lung tissue and lung and liver MPO activity were significantly increased in Galphai2 (-/-) compared to WT mice. PMID: 21255617 Variations in Gnai2 and Rgs1 expression affect chemokine receptor signaling and the organization of secondary lymphoid organs. PMID: 20508603 Data suggest that Galphai-TNFAIP8-mediated rescue of pre-oncogenic cells enhances progression to oncogenic transformation, providing a selective target to inhibit cellular transformation. PMID: 20607800 The absence of inhibitory G-protein signaling mediated through Galpha(i2) is a substrate for ventricular arrhythmias. PMID: 20495013 G alpha i2(-/-) mice are deficient in B-1a B cells, splenic marginal zone B cells, and their immediate precursor, T2 B cells, with resultant in vitro functional deficiency in LPS-induced proliferation and IL-10 production. PMID: 12574334 Gi2 has a role in regulating mitogenic activity in the neural progenitor cells of developing brain PMID: 15272018 Alveolar macrophages use a Galphai2-dependent signal for modulating the two opposing Fc gamma receptors, FcgammaRIIB and FcgammaRIII, in the initiation of the inflammatory cascade in the lung Arthus reaction. PMID: 15728518 Constitutively active Galphai2 occludes the transient suppression of synaptic transmission by mGluR2. PMID: 16606834 Loss of Regulators of G protein signaling (RGS) actions at Galpha(i2) produces a dramatic and pleiotropic phenotype which is more evident than the phenotype seen for individual RGS protein knockouts. PMID: 16943428 These data demonstrate that G(i2) and G(i1/3) proteins are both involved and differentially regulate splenocyte inflammatory cytokine and chemokine production in a highly Gi isoform specific manner in response to LPS and Gram-positive microbial stimuli. PMID: 16962188 Galphai2 signaling events are not required for the development of the protective immune responses against S. stercoralis; however, Galphai2 is essential for the recruitment of neutrophils required for host-dependent killing of larvae. PMID: 17242370 This study details distinct G alpha(i2) and G alpha(i3) effects on chemokine receptor CXCR3-mediated signaling. PMID: 17289675 Gnai2 knockout mice show significantly reduced intestinal IL-11 production. Suggest MAP kinases, PGE2 and cAMP mediated signaling involved. PMID: 17332478 specific Galpha(i2)-mediated signaling between endothelial cells and leukocytes is required for the extravasation of leukocytes and for tissue-specific accumulation PMID: 17360531 Ghrelin attenuates glucose-induced insulin release via pertissigen-sensitive Galpha(i2)-mediated activation of potassium channels. PMID: 17575083 Gnai2-deficient T cells exhibit significant defects in chemokine receptor signaling and illustrate how signals that modulate the level of Galphai2 present in lymphocytes directly affect the capacity of lymphocytes to respond to chemokines. PMID: 17579064 Neutrophil Galpha(i2) is necessary for chemokine-induced arrest. PMID: 17699741 Transgenic mice on a high fat diet which have this gene exhibit enhanced insulin sensitivity and increased glucose tolerance. PMID: 17928396 results indicate that the upregulation of Gi2 in ischemic myocardium appears to be protective in inducing cell survival pathways to prevent myocyte death in response to ischemia/reperfusion injury PMID: 18316484 Absence of either Galphai2 or Galphai3 significantly abrogated thymic homing, with an effect of Galphai3 being greater than that of Galphai2. PMID: 18501427 These observations underscore interplay between Galphai2 and Galphai3 and potentially provide a novel strategy to prevent GVHD by blocking T cell homing at early stages and T effector cell trafficking at later time points. PMID: 18521956 Report role of Gnai2 in the control of in vivo heart rate dynamics. PMID: 18832081 In Galphai2(-/-) and interleukin-10(-/-) transgenic mice susceptible to immune-mediated colitis and inflammation-associated adenocarcinoma, similar levels of peripheral leukocyte and erythroblast genotoxicity were also observed. PMID: 19487293 Young Gialpha2-/- colonic epithelium has normal MMR expression but selectively loses MLH1 and consequently PMS2 expression following inflammation. PMID: 19638594

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.