Recombinant Mouse Glutathione S-Transferase P 1 (GSTP1) Protein (His-SUMO)

Name: Recombinant Mouse Glutathione S-Transferase P 1 (GSTP1) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-04694P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Mouse Glutathione S-Transferase P 1 (GSTP1) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P19157
Target Symbol	GSTP1
Synonyms	Gstp1; GstpibGlutathione S-transferase P 1; Gst P1; EC 2.5.1.18; GST YF-YF; GST class-pi; GST-piB; Preadipocyte growth factor
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	PPYTIVYFPVRGRCEAMRMLLADQGQSWKEEVVTIDTWMQGLLKPTCLYGQLPKFEDGDLTLYQSNAILRHLGRSLGLYGKNQREAAQMDMVNDGVEDLRGKYVTLIYTNYENGKNDYVKALPGHLKPFETLLSQNQGGKAFIVGDQISFADYNLLDLLLIHQVLAPGCLDNFPLLSAYVARLSARPKIKAFLSSPEHVNRPINGNGKQ
Expression Range	2-210aa
Protein Length	Full Length of Mature Protein
Mol. Weight	39.5kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). Participates in the formation of novel hepoxilin regioisomers. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.
Subcellular Location	Cytoplasm. Mitochondrion. Nucleus.
Protein Families	GST superfamily, Pi family
Database References	KEGG: mmu:14870 STRING: 10090.ENSMUSP00000129565 UniGene: PMID: 27058114 GSTP can exert redox regulation in the oxidative ER environment and indicate that, within the ER, GSTP influences the cellular consequences of the UPR through S-glutathionylation of a series of key interrelated proteins. Antioxid. PMID: 26838680 our study reveals the novel role of GSTP1 in regulation of iNOS by affecting S-nitrosylation, dimerization, and stability. PMID: 26361746 These observed differences contribute to our understanding of how genetic ablation of GSTP causes different levels of myeloproliferation and migration [corrected] PMID: 25216273 GSTP1 expression in retina increases with developmental age in mice and accompanies murine retinal maturation. PMID: 24664677 GSTP knock-out mice display decreased ubiquitination capacity and overall increased susceptibility to oxidative stress. PMID: 23129554 Report increased GSTP1 in airways of asthmatic mice. PMID: 23647087 DNIC storage function of GST P1-1 and ability of MRP1 to efflux DNICs are vital in protection against NO cytotoxicity PMID: 22084240 Gst-pi expression of the prostate cancers are dependent on metastatic site. PMID: 21748757 GSTP plays a major role in carcinogenesis distinct from its role in detoxification and provides evidence that the enzyme is a key determinant of the proinflammatory tumor environment. PMID: 21975931 This study analysed the kinetic behaviour of all the various cysteine to alanine mutants of Gstp1. These mutations introduce cooperativity and a novel 'ping pong' mechanism in the case of the C169A mutant. PMID: 21134126 Sequential catalysis of Aldr1 by glutathione S-transferase P and glutaredoxin may be a general redox switching mechanism that regulates the reduction of protein sulfenic acids to cysteines regulates the reduction of protein sulfenic acids to cysteines PMID: 20538586 role in activating Pi-class glutathione S-transferase gene in conjunction with androgens PMID: 12023900 Differential expression of GSTpi contributes to the sensitivity to xenobiotics in the substantia nigra and may influence the pathogenesis of reactive oxygen species-induced neurological disorders including Parkinson's disease. PMID: 17267597 GSTP1 attenuates the severity of allergic airways disease. However, the efficacy of GSTP1 correlated with mouse strain-dependent variation in Gstp1 expression. PMID: 18787219 Results suggest that GSTpi expression may be part of the mechanism underlying the ability of glial cells to elicit protection against the mechanisms involved in MPTP-induced neuronal death. PMID: 18792812 GSTP1 prevented LPS-induced TNF-alpha, IL-1beta, MCP-1 and NO production. PMID: 18962899 Catalysis of S-glutathionylation at low pK cysteines in proteins is a novel property for GSTpi PMID: 18990698

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.