Recombinant Mouse Formyl Peptide Receptor 2 (FPR2) Protein (His-GST&Myc)

Name: Recombinant Mouse Formyl Peptide Receptor 2 (FPR2) Protein (His-GST&Myc)
Brand: Beta LifeScience
SKU: BLC-02207P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Description	Recombinant Mouse Formyl Peptide Receptor 2 (FPR2) Protein (His-GST&Myc) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	O88536
Target Symbol	FPR2
Synonyms	Fpr2; Fpr-rs2; Formyl peptide receptor 2; Formylpeptide receptor-related sequence 2; Lipoxin A4 receptor-like protein; N-formylpeptide receptor-like 2
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-10His-GST&C-Myc
Target Protein Sequence	MESNYSIHLNGSEVVVYDSTISRVLWILS
Expression Range	1-29aa
Protein Length	Partial
Mol. Weight	33.3 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	High affinity receptor for N-formyl-methionyl peptides (FMLP), which are powerful neutrophil chemotactic factors. Stimulates chemotaxis in immune cells to site of infection or tissue damage upon recognition of several ligands, such as FMLP, or ligand involved in cell damage, disease or inflammation. Receptor for the chemokine-like protein FAM19A5, mediating FAM19A5-stimulated macrophage chemotaxis and the inhibitory effect on TNFSF11/RANKL-induced osteoclast differentiation.
Subcellular Location	Cell membrane; Multi-pass membrane protein.
Protein Families	G-protein coupled receptor 1 family
Database References	KEGG: mmu:14289 STRING: 10090.ENSMUSP00000065799 UniGene: PMID: 29127186 this study shows that formyl peptide receptor activation inhibits the expansion of effector T cells and synovial fibroblasts and attenuates joint injury in models of rheumatoid arthritis PMID: 29879657 The influx of leukocytes into the peritoneum of WT and mFpr2(-/-) mice was analyzed. We demonstrate that mFpr2 is specifically activated by phenol-soluble modulin (PSM) peptides, and they represent the first secreted pathogen-derived ligands for the mFpr2. PMID: 28855276 Study showed significantly increased expression of FPR1 and FPR2, during differentiation of neural stem cells (NSCs). The activation of FPRs promotes NSCs to differentiate into neurons with more primary neurites and branch points and longer neurites per cell. Meanwhile, this activation also inhibits the differentiation of NSCs into astrocytes. PMID: 28303030 activation of Fpr2 in bone marrow after WKYMVm treatment provides cardiac protection through mobilization of circulating angiogenic cells after myocardial infarction. PMID: 27790799 we suggest that Fpr2-mediated signaling in follicular dendritic cells plays a key role in germinal center maintenance in Peyer's patches PMID: 27974458 FAM3D plays a role in gastrointestinal homeostasis and inflammation through its receptors FPR1 and FPR2. PMID: 26966188 antagonist pretreatment or gene silencing of the RvD1 receptor, ALX/FPR2, abrogated the anti-inflammatory and pro-resolving actions of RvD1. These data indicate that RvD1 ameliorates IR-induced liver injury, and this protection is associated with enhancement of M2 polarization and efferocytosis via ALX/FPR2 activation PMID: 27317426 a novel FPR2 agonist, the proteolytically stable alpha-peptide/beta-peptoid hybrid Lau-((S)-Aoc)-(Lys-betaNphe)6-NH2 (F2M2), showing comparable potency in activating human and mouse neutrophils by inducing a rise in intracellular Ca(2+) concentration and assembly of the superoxide-generating NADPH oxidase. PMID: 27422818 We conclude that ANX-A1 is an important regulator of mast cell reactivity to compound 48/80 exerting a negative feedback effect through a mechanism that depends at least partly on the FPR receptor PMID: 26803520 annexin A1- formyl peptide receptor 2 pathway mediated the insulin resistance of skeletal muscle, as well as systemic insulin sensitivity. PMID: 25616869 Deficiency of formyl peptide receptor 2 is associated with increased inflammation and enhanced liver injury after LPS-stimulation PMID: 24956481 AnxA1 and Fpr2 have a critical role in the manifestation of adrenal insufficiency in this LPS-induced model, through regulation of cholesterol ester storage PMID: 25818588 Ldlr(-/-)xFpr2(-/-) mice exhibited delayed atherosclerosis development and less macrophage infiltration compared with Ldlr(-/-)xFpr2(+/+) mice. PMID: 25341894 Compared with wild-type mice, Fpr2/3(-/-) animals exhibited exacerbation of disease severity, including hypothermia and cardiac dysfunction. PMID: 25512512 Fpr1/2 are critical for normal healing of the sterile skin wound by mediating the first wave of neutrophil infiltration. PMID: 24603667 FPR1 and FPR2 play an important role in the innate immune responses against Streptococcus pneumoniae within the central nervous system and the lack of the receptors leads to a dysregulation of the inflammatory response compared with wild-type mice. PMID: 24863484 Oxidized low-density lipoprotein stimulates macrophages, resulting in chemotactic migration, TNF-alpha production, and foam cell formation via FPR2 signaling. PMID: 24361884 During ischemia, neutrophil Fpr2/3 controls platelet/neutrophil aggregates with the rapid generation of circulating LXA4, which in turn modulates downstream vascular inflammatory responses evident during the reperfusion phase. PMID: 23733341 FPR2 is not activated by lipoxin (LX)A; the molecular mechanism by which LXA functions still needs to be identified. PMID: 23643932 Fpr2 mediated foam cell formation PMID: 23500463 FPR2 is critical in mediating homeostasis, inflammation, and epithelial repair processes in the colon. PMID: 23454745 Results indicate that the AnxA1/FPR2 system has an important role in effecting the resolution of cerebral inflammation in sepsis and may provide a novel therapeutic target. PMID: 22964301 The mechanism involved impaired early neutrophil recruitment to the liver with Fpr2 being sole receptor for neutrophils to sense Listeria chemoattractant signals and for production of bactericidal superoxide. PMID: 23139859 These results suggest that Fpr2 plays an important role in host defense against implanted LLC by sustaining macrophages in an M1 phenotype with more potent antitumor activities. PMID: 23139214 results suggest that microvascular endothelial cells express mFPR2 which can be upregulated by proinflammatory factors IL-1beta and LPS through JNK and NF-kappaB related signaling pathways PMID: 21761148 The conclusions drawn from these experiments are that neither compound 43 nor LXA4 works as FPR2 agonists in neutrophils, findings of importance for a proper interpretation of results obtained with these compounds as regulators of inflammation. PMID: 21535079 The recognition of Abeta by the formylpeptide chemotactic receptor 2 seems to be a starting point of the signaling cascade inducing an inflammatory state in AD. PMID: 20456016 Studies show that inflammation was more marked in Fpr2(-/-) mice. PMID: 20107188 This study reveals a critical role for mFPR2 in the progression of allergic airway inflammation and immune responses. PMID: 20200280 By selectively up-regulating FPR2 in microglia, TNF alpha has the capacity to amplify host response in inflammatory diseases in the central nervous system. PMID: 12270697 TLR9 may play an important role in promoting microglial recognition of Abeta42 by up-regulating the expression of the G-protein- coupled receptor mFPR2 PMID: 16219804 mouse neutrophils, which like macrophages and dendritic cells express Fpr2, responded to human and mouse F2L in both calcium flux and chemotaxis assays PMID: 17237393 the effect of IFN-g and its synergy with CD40L on mFPR2 expression in microglia was mediated in part by TNF-alpha; IFN-g and CD40L may profoundly affect microglial cell responses in the pathogenic process in which mFPR2 agonist peptides are elevated PMID: 17237425 IL-10 may affect the pathogenic process of Alzheimer's disease by up-regulating mFPR2 and thus favoring the recognition and internalization of Abeta(42) by activated microglial cells PMID: 17544285 TLR2 and NOD2 cooperate to up-regulate the expression of mFPR2 and therefore, may actively participate in the pathogenic processes of brain inflammation and neurodegenerative diseases. PMID: 18299458 (Poly(I:C)), which is a specific TLR3 ligand, and Imiquimod (R837), which is a specific TLR7 ligand, when used alone, each increased MAPK-dependent functional mFPR2 expression in microglial cells. PMID: 19559490

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.