Recombinant Mouse Forkhead Box Protein P3 (FOXP3) Protein (His&Myc)

Name: Recombinant Mouse Forkhead Box Protein P3 (FOXP3) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-07171P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Forkhead Box Protein P3 (FOXP3) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q99JB6
Target Symbol	FOXP3
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	MPNPRPAKPMAPSLALGPSPGVLPSWKTAPKGSELLGTRGSGGPFQGRDLRSGAHTSSSLNPLPPSQLQLPTVPLVMVAPSGARLGPSPHLQALLQDRPHFMHQLSTVDAHAQTPVLQVRPLDNPAMISLPPPSAATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPRSGTPRKDSNLLAAPQGSYPLLANGVCKWPGCEKVFEEPEEFLKHCQADHLLDEKGKAQCLLQREVVQSLEQQLELEKEKLGAMQAHLAGKMALAKAPSVASMDKSSCCIVATSTQGSVLPAWSAPREAPDGGLFAVRRHLWGSHGNSSFPEFFHNMDYFKYHNMRPPFTYATLIRWAILEAPERQRTLNEIYHWFTRMFAYFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDEFEFRKKR
Expression Range	1-417aa
Protein Length	Full Length of Mature Protein
Mol. Weight	53.5 kDa
Research Area	Cell Biology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2. Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7. Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1. Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development. Inhibits the transcriptional activator activity of RORA. Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4.
Subcellular Location	Nucleus. Cytoplasm.
Database References	KEGG: mmu:20371 STRING: 10090.ENSMUSP00000041953 UniGene: PMID: 30003817 Foxp3 vaccine promotes an immune response against tumor. PMID: 29124314 Unexpectedly, although dispensable for FOXP3 expression and for the homeostasis and suppressive function of thymus-derived Treg cells, CREB negatively regulates the survival of TGF-beta-induced Treg cells, and deletion of CREB resulted in increased FOXP3+ Treg cells in the intestine and protection in a colitis model PMID: 29050947 This study reports on systematic alanine-scan mutagenesis of FoxP3, assessing mutational impacts on DNA binding and transcriptional activation or repression. PMID: 29269391 Foxp3 is essential for beneficial outcome of the microglial response and depends upon signalling by the immunoglobulin CD200 through its receptor (CD200R) PMID: 27731341 Tbet is a critical modulator of FoxP3 expression in autoimmune graft-versus-host disease PMID: 28473623 Generation of RORgammat(+) antigen-specific T17 regulatory cells from Foxp3(+) precursors in autoimmunity has been described. PMID: 28978473 KLRG1 expression identifies short-lived Foxp3(+) Treg effector cells with functional plasticity in islets of NOD mice. PMID: 28850267 Novel regulatory T-cells that are induced by B cells and do not express Foxp3 and IL-10 alleviate intestinal inflammation in vivo. PMID: 27581189 the findings suggested that excreted-secreted antigens restricted Foxp3 expression by inhibiting TGFssRII/Smad2/Smad3/Smad4 signalling, ultimately resulting in abortion. PMID: 28300338 Data (including data from studies using transgenic mice) suggest that a single oral dose of vitamin A (1) amplifies tolerogenic activity of dendritic cells migrating to lymphoid tissue and (2) up-regulates expression of Foxp3 and Cd44 in co-cultures of dendritic cells and CD4+ lymphocytes. (Foxp3 = forkhead box P3; Cd44 = homing receptor) PMID: 28917953 Flicr, a long noncoding RNA, modulates Foxp3 expression and autoimmunity. PMID: 28396406 Dendritic cells have the capacity to express Foxp3, which can be upregulated by exposure to Staphylococcal enterotoxin B. PMID: 27861999 the Treg transcription factor Foxp3 reprograms T cell metabolism by suppressing Myc and glycolysis, enhancing oxidative phosphorylation, and increasing nicotinamide adenine dinucleotide oxidation. PMID: 28416194 Data suggest that genetic or environmental factors that even moderately affect the expression of both PD-1 and FoxP3 can cause life-threatening autoimmune diseases by disrupting the T-cell homeostasis. PMID: 27410049 Control of Regulatory T Cell Development by the Transcription Factor Foxp3. PMID: 28115586 Foxp3 Programs the Development and Function of CD4+CD25+ Regulatory T Cells. PMID: 28115587 An Essential Role for Scurfin in CD4+CD25+ T Regulatory Cells. PMID: 28115588 Mechanistic analysis indicated that E47 activated expression of the transcription factor Spi-B and the suppressor of cytokine signaling 3 (SOCS3), which both downregulated Foxp3 expression. These findings demonstrate that the balance of Id3 and E47 controls the maintenance of Foxp3 expression in Treg cells and, thus, contributes to Treg cell plasticity. PMID: 27974197 Foxp3 expression in lung epithelial cells, and not in Treg cells, inhibited OVA- and cockroach extract-induced asthma. PMID: 27633092 Regulatory T cells have a high apoptosis rate ex vivo correlating with low c-FLIP expression. PMID: 28052242 Forkhead box P3 protein (FoxP3) acts in multimodal fashion to directly activate or repress transcription, in a context- and partner-dependent manner, to govern Treg cell phenotypes. PMID: 28892470 Membrane-bound DKK-1 is a novel Foxp3 postive Treg cell derived mediator to maintain immunological tolerance in T-cell-mediated autoimmune colitis. PMID: 28556921 A384T mutation associated with autoimmune IPEX syndrome, induces a distinctive tissue-restricted inflammation by specifically impairing the ability of Treg cells to compete with pathogenic T cells PMID: 28778586 Systemic NRF2 activation by Keap1 knockdown alleviates multiple-organ inflammation and improves the survival rate of Sf mice. PMID: 28507037 CTLA-4 expressed by FOXP3(+) regulatory T cells prevents inflammatory tissue attack and not T-cell priming in arthritis. PMID: 28497863 Inhibition of IL-27p28 also enhanced the suppressive capacity of adoptively transferred CD4(+) nTregs by increasing the stability of Foxp3 expression. PMID: 27488350 This study suggests that the epigenetic modification of FOXP3 gene is involved in the pathogenesis of EAE. PMID: 28320131 Foxp3 expression is unstable in transforming growth factor beta (TGF-beta)-induced Tregs (iTregs), while stable in thymus-derived Tregs (tTregs). To stabilize Foxp3 expression in iTregs, we introduced dCas9-TET1CD (dCas9 fused to the catalytic domain (CD) of ten-eleven translocation dioxygenase 1 (TET1), methylcytosine dioxygenase) and dCas9-p300CD (dCas9 fused to the CD of p300, histone acetyltransferase) with guide RNA. PMID: 28503202 Our findings illustrate that deregulated myelopoiesis in Sf mice is mainly caused by the inflammatory reaction resulting from the lack of Treg cells. PMID: 27130185 Increased methylation of "Foxp3 promoter" in Treg cells leads to impaired Treg suppressive function in biliary atresia. PMID: 27659419 this study shows that inflammatory signals and Foxp3 balance mTORC1 signaling and glucose metabolism to control the proliferation and suppressive function of Treg cells PMID: 27695003 results demonstrate that Tpl2 promotes inflammation in part by constraining FoxP3 expression and Treg immunosuppressive functions. PMID: 27261457 Increased numbers of CD4(+)CD45RA(-)FoxP3(low) cells may cause an imbalance between Treg and Th17 cells that is mainly localized to the colon. PMID: 27895423 this study shows that pretreatment with sodium selenite prevents experimental colitis by restoring Foxp3 excretion PMID: 27533281 Study highlights the importance of IL-23R expression level and the instability of Foxp3+ regulatory T cells in the development of inflammatory bowel diseases. PMID: 27498708 indirubin administration effectively suppressed CD4(+) T cell infiltration in the colon of DSS-induced UC mice and promoted the generation of Foxp3-expressing regulatory T cells PMID: 27396532 The data suggest that RORgt expression in Tregs contributes to an optimal suppressive capacity during gut-specific immune responses, rendering Foxp3-RORgt T cells as an important effector Treg subset in the intestinal system. PMID: 26307665 The ex vivo expression of transforming growth factor (TGF)-beta and factor Foxp3 were significantly higher in splenocytes of the experimental mice than the basal expression observed in the control cells. PMID: 25850927 down-regulated expression in abortion mouse model PMID: 26474535 These observations reveal an unexpected differential role of DJ-1 in the development of nTregs and iTregs. PMID: 26634899 An IL-27/Lag3 axis enhances Foxp3+ regulatory T cell-suppressive function and therapeutic efficacy. PMID: 26013006 the presence of IL-2 during the in vitro generation of iTreg cells confers resistance to Th17 conversion but that IL-2 and retinoic acid (RA) cooperate to maintain Foxp3 expression following stimulation under Th17-polarizing conditions PMID: 26583087 Foxp3 has a rapid turn over in Treg partly controlled at the transcriptional level by the JAK/STAT pathway PMID: 27077371 NZW Tregs were less sensitive to limiting doses of trophic cytokines, IL-2 and -33, for population homeostasis and for maintenance of FoxP3 expression PMID: 26768846 Data show that tubacin can upregulate forkhead box P3 protein (Foxp3) expression of CD4(+) CD25(+) Tregs and enhances their cellular immunosuppressive capability. PMID: 26927553 YY1 interrupts Foxp3-dependent target gene expression by physically interacting with Foxp3 and by directly binding to the Foxp3 target genes. PMID: 26892542 the stability of Foxp3 expression is markedly compromised in T reg cells from Tet2/Tet3 double-deficient mice. PMID: 26903244 Il10(-/-) Treg cells showed reduced steady-state Foxp3 expression, and polyclonal stimulation caused greater loss of Foxp3 PMID: 26224007 Antigen receptor-mediated depletion of FOXP3 in induced regulatory T-lymphocytes via PTPN2 and FOXO1 PMID: 26815406

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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