Recombinant Mouse EpCAM Protein (C-Fc)

Name: Recombinant Mouse EpCAM Protein (C-Fc)
Brand: Beta LifeScience
SKU: BL-0203NP
Availability: InStock

BL-0203NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Car-nk targets proteins, High-quality recombinant proteins, Recombinant cell therapy targets for car-t research

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Product Overview

Description	Recombinant Mouse Epithelial Cell Adhesion Molecule is produced by our Mammalian expression system and the target gene encoding Gln24-Thr266 is expressed with a human IgG1 Fc tag at the C-terminus.
Accession	Q99JW5
Synonym	17-1A; 323/A3; ACSTD1;CD326;EGP-2; EGP314; EGP40; EpCAM; MOC31; TACST-1; TACSTD1; TROP1;
Gene Background	Epithelial Cellular Adhesion Molecule (Ep-CAM), also known as EGP314, mEGP314, Protein 289A, Tumor-associated calcium signal transducer 1, CD326, belongs to the EPCAM family. Its’ monomer subunit structure interacts with phosphorylated CLDN7. Ep-CAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. It plays a role in embryonic stem cells proliferation and differentiation. It also up-regulates the expression of FABP5, MYC and cyclins A and E. The post-translational modification glycosylation at Asn-198 is crucial for protein stability.
Molecular Mass	54.8 KDa
Apmol Mass	60-80 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Subcellular Location	Lateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction.
Protein Families	EPCAM family
Database References	KEGG: mmu:17075 STRING: 10090.ENSMUSP00000061935 UniGene: PMID: 29958886 EpEX/EpCAM are sufficient to reprogram fibroblasts into iPSCs with only Oct4 or Klf4. PMID: 28157205 extracellular vesicles tend to localize in the intestinal tract associated with epithelial cell adhesion molecule PMID: 27721471 Data show that EpCAM-expressing proliferating ductal cells (PDC) could be a cellular origin of hepatocellular carcinoma (HCC), suggesting the existence of stem/progenitor-derived hepatocarcinogenesis. PMID: 28951464 activation of hepatic H19RNA promoted cholestatic liver fibrosis through the ZEB1/EpCAM signaling pathway PMID: 28407375 These results identify EpCAM as a substrate of matriptase and link HAI-2, matriptase, EpCAM, and claudin-7 in a functionally important pathway that causes disease when it is dysregulated. PMID: 28094766 EpCAM appears to differentially regulate Langerhans cell mobility/migration in the setting of limited inflammation as compared with the intense inflammation triggered by contact sensitizers PMID: 27106675 Knock-down EpCAM cell model of congenital tufting enteropathy was developed. In vivo inducible mouse model was developed resulting in mutant EpCAM protein. PMID: 25482158 in addition to converting to cholangiocyte-like cells, Sox9(+)EpCAM(-) cells provide luminal space near expanded ductular structures to prevent deterioration of the injuries and potentially supply new hepatocytes to repair damaged tissues PMID: 24482234 EpCAM is a highly conserved protein present in fishes, amphibians, reptiles, birds, marsupials, and placental mammals, and is subject to shedding, gamma-secretase-dependent regulated intramembrane proteolysis, and proteasome-mediated degradation. PMID: 24009667 Suggest pivotal role of EpCAM in intestinal epithelial structure and integrity, with mutations resulting in congenital tufting enteropathy. PMID: 24337010 mTrop1/Epcam knockout mice develop congenital tufting enteropathy through dysregulation of intestinal E-cadherin/beta-catenin. PMID: 23209569 EpCAM contributes to formation of intestinal barrier by recruiting claudins to cell-cell junctions. PMID: 22819673 These results conclusively link EpCAM expression to Langerhan cell (LC) motility/migration and LC migration to immune regulation. PMID: 22411813 Results show that SSCs are the most concentrated in CD9(+)EPCAM(low/-) population and also suggest that EPCAM plays an important role in progenitor cell amplification in the mouse spermatogenic system. PMID: 21858196 EpCAM could contribute substantially to the pathogenesis of lung cancer. PMID: 21535318 EpCAM was required for differentiation or survival of parietal trophoblast giant cells, normal development of the placental labyrinth and establishment of a competent maternal-fetal circulation. PMID: 20046825 analysis of EpCAM expression in normal, non-pathological tissues PMID: 17981779 The spatiotemporal expression pattern of EpCAM changes during nephrogenesis PMID: 18025791 These data suggest that EpCAM is involved in signal transduction triggering several intracellular signalling pathways using tumor cell lines in colorectal and lung cancer. PMID: 19002182 EpCAM(+) cells isolated from injured liver proliferate to form colonies in vitro, and the clonally expanded cells differentiate into hepatocytes and cholangiocytes, suggesting that the oval cell fraction contains potential HSCs. PMID: 19429791 expression of EpCAM and DLK1 suggests the developmental pathways of mouse liver progenitors PMID: 19527784

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.