Recombinant Mouse D-Amino-Acid Oxidase (DAO) Protein (His)

Name: Recombinant Mouse D-Amino-Acid Oxidase (DAO) Protein (His)
Brand: Beta LifeScience
SKU: BLC-01071P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins, Recombinant proteins fall special offers - full-length proteins

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Product Overview

Description	Recombinant Mouse D-Amino-Acid Oxidase (DAO) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P18894
Target Symbol	DAO
Synonyms	(DAAO)(DAMOX)(DAO)
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	MRVAVIGAGVIGLSTALCIHERYHPTQPLHMKIYADRFTPFTTSDVAAGLWQPYLSDPSNPQEAEWSQQTFDYLLSCLHSPNAEKMGLALISGYNLFRDEVPDPFWKNAVLGFRKLTPSEMDLFPDYGYGWFNTSLLLEGKSYLPWLTERLTERGVKLIHRKVESLEEVARGVDVIINCTGVWAGALQADASLQPGRGQIIQVEAPWIKHFILTHDPSLGIYNSPYIIPGSKTVTLGGIFQLGNWSGLNSVRDHNTIWKSCCKLEPTLKNARIVGELTGFRPVRPQVRLEREWLRHGSSSAEVIHNYGHGGYGLTIHWGCAMEAANLFGKILEEKKLSRLPPSHL
Expression Range	1-345aa
Protein Length	Full Length
Mol. Weight	42.7 kDa
Research Area	Metabolism
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
Subcellular Location	Peroxisome.
Protein Families	DAMOX/DASOX family
Database References	KEGG: mmu:13142 STRING: 10090.ENSMUSP00000107911 UniGene: PMID: 29532193 findings reveal that DAO is a microbiota-regulated host innate immune factor that modulates growth of both pathogens and commensals, primarily in the small intestine PMID: 27670111 some significant in vivo effects of DAOR199W on motor neuron function (i.e. kyphosis and loss of motor neurons) were detected which were most marked in females and could contribute to the earlier onset of neurological signs in double transgenic females compared to SOD1G93A littermates, highlighting the importance of recognizing gender effects present in animal models of ALS PMID: 29194436 Long-term spatial memory is unaltered in Dao-/- mice. PMID: 26833794 Dao-/- mice demonstrated enhanced spatial recognition memory and no sleep or circadian rhythm disruption. PMID: 25816902 In d-Amino acid oxidase (-/-) mice, approximately twice as many Dopamine-like neurons fired in a bursting pattern than in DAO(+/-) or DAO(+/+) mice. Data provide the first direct evidence that DAO modulates VTA DA neuron activity PMID: 25040393 The retinal levels of d-serine are tightly controlled during the first week of postnatal life and vary according to deficiencies in either DAO/ PMID: 25083578 DAO deficiency reduces D-serine level in retina and increases NMDA receptor expression. PMID: 24068757 D-amino acids and DAO have pivotal neurotransmission functions in the central nervous system. (Review) PMID: 22892863 D-Amino acid oxidase-induced oxidative stress, 3-bromopyruvate and citrate inhibit angiogenesis, exhibiting potent anticancer effects. PMID: 22802136 results suggest that DAO, together with myeloperoxidase, may play an important role in antibacterial systems in mammals PMID: 22271930 DAO has a pivotal role in motoneuron degeneration through D-serine regulation, with inactivity of DAO being a common feature between the mSOD1 ALS mouse model and mutant DAO-associated familial ALS. PMID: 22203986 Results confirm the role of D-amino acid oxidase (DAO) in D-serine metabolism, and provide a tool to investigate DAO, an enzyme currently of considerable interest in the pathophysiology and therapy of schizophrenia. PMID: 20828614 data show enhanced PPI responses and enhanced PPI-disruptive effects of NMDA antagonists in DAO-/- mice . PMID: 20933022 Familial amyotrophic lateral sclerosis is associated with a mutation in D-amino acid oxidase PMID: 20368421 Hypofunctional Dao 1 mutation elevated brain levels of D-serine. Mice with both Dao1 and Grin 1 mutations had improved social approach spatial memory retention, reversal of latent inhibition, and partial normalized startle responses. PMID: 19751394 These results suggest that the abundant D-amino-acids in the mutant mouse brain facilitate hippocampal LTP and spatial learning. PMID: 15996778 Lacks DAO activity and shown for the first time it has increased occupancy of the NMDAR glycine site due to elevated extracellular D-serine levels and has enhanced NMDAR function in vivo. PMID: 16843004 Its physiological role is in metabolizing endogenous and exogenous D-amino acids. PMID: 18516913 D-amino acid oxidase may be a promising therapeutic target to improve cognitive flexibility and inhibitory learning in psychiatric disorders such as schizophrenia and anxiety syndromes. PMID: 19117914

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.