Recombinant Mouse Complement Component Receptor 1-Like Protein (CR1L) Protein (His)

Name: Recombinant Mouse Complement Component Receptor 1-Like Protein (CR1L) Protein (His)
Brand: Beta LifeScience
SKU: BLC-02515P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Cr1l.

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Complement Component Receptor 1-Like Protein (CR1L) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q64735
Target Symbol	CR1L
Synonyms	Cr1l; Crry; CryComplement component receptor 1-like protein; Complement regulatory protein Crry; Protein p65
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	ELRGGLGKHGHTVHREPAVNRLCADSKRWSGLPVSAQRPFPMGHCPAPSQLPSAKPINLTDESMFPIGTYLLYECLPGYIKRQFSITCKQDSTWTSAEDKCIRKQCKTPSDPENGLVHVHTGIQFGSRINYTCNQGYRLIGSSSAVCVITDQSVDWDTEAPICEWIPCEIPPGIPNGDFFSSTREDFHYGMVVTYRCNTDARGKALFNLVGEPSLYCTSNDGEIGVWSGPPPQCIELNKCTPPPYVENAVMLSENRSLFSLRDIVEFRCHPGFIMKGASSVHCQSLNKWEPELPSCFKGVICRLPQEMSGFQKGLGMKKEYYYGENVTLECEDGYTLEGSSQSQCQSDGSWNPLLAKCVSRSISG
Expression Range	41-405aa
Protein Length	Partial
Mol. Weight	44.5 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. Also acts as a decay-accelerating factor, preventing the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Plays a crucial role in early embryonic development by maintaining fetomaternal tolerance. Also acts as a costimulatory factor for T-cells which favors IL-4 secretion.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Protein Families	Receptors of complement activation (RCA) family
Database References	KEGG: mmu:12946 UniGene: PMID: 28348233 this study has provided direct evidence for a role of Crry in controlling spontaneous alternative pathway complement activation on renal tubular epithelial cells. PMID: 24850152 Crry costimulation enhanced the in vitro expansion of natural Treg cells while maintaining their phenotypic and suppressive properties. Crry-expanded Treg cells decreasing antigen-dependent secretion of cytokines. PMID: 21380996 Stmn2 and the chromosome 14 Rarb region, but not Cr1 or Clu or Picalm, have roles in prion disease. PMID: 21151910 Crry is clearly present in the placentas from spontaneous abortions in mice. PMID: 20484840 Crry is the only membrane-bound complement regulator expressed on the basolateral surface of murine renal tubular epithelial cells under in vitro culture conditions. PMID: 20675597 Data show that a single-nucleotide polymorphism in the first short consensus repeat of Sle1c Crry introduced a novel N-linked glycosylation site likely responsible for structural alteration. PMID: 20660348 mesangial cell Crry limits complement activation and subsequent neutrophil recruitment in the setting of local immune complex deposition. PMID: 19740350 Long-term complement blockade with Crry, a C3 convertase inhibitor overexpressed in a transgenic mouse line, reduces renal disease and improves survival in lupus-prone MRL/lpr mice. PMID: 11907125 the role of crry in regulating complement regulation PMID: 11915940 Crry is indispensable for erythrocyte protection from spontaneous complement attack PMID: 11986227 Crry plays a more dominant role than DAF in regulating the alternative pathway of complement C3 activation, whereas DAF and Crry are equally effective in preventing antibody-induced runaway complement activation. PMID: 12393518 X-ray and neutron scattering was performed on recombinant rat Crry containing the first five SCR domains (rCrry) and mouse Crry with five SCR domains conjugated to the Fc fragment of mouse IgG1 (mCrry-Ig) PMID: 12767833 Mice transgenic for murine complement inhibitor Crry are largely protected from developing collagen-induced arthritis. PMID: 12902517 Data demonstrate that transgenic mice with astrocyte-targeted expression of the soluble complement inhibitor sCrry have a significantly reduced neurologic impairment and improved blood-brain barrier function after closed head injury. PMID: 12973023 The dual function of Crry as a complement regulatory protein and as a T cell costimulator illustrates the importance of complement regulatory proteins as links between innate and adaptive immunity. PMID: 16301324 Results suggest that altered expression of Crry within the tubular epithelium appears to be a critical factor permitting activation of the alternative pathway of complement after ischemia/reperfusion. PMID: 16444293 both a high level expression and a more potent anti-alternative pathway complement activity of Crry contributed to its indispensable role on murine erythrocytes PMID: 17015743 Our data suggest complement provides some protection of mature oligodendrocytes during cuprizone treatment but may be critical for subsequent remyelination events. PMID: 17674370 DAF and Crry double deficiency led to rapid clearance of platelets from circulation in a complement- and macrophage-dependent manner; redundant role of DAF and Crry in platelet survival PMID: 18524992 Membrane complement regulator Crry helps to maintain homeostasis of the complement alternative pathway (AP) and demonstrates that Crry-deficient mice can be rescued on a partial as well as on a complete factor B- or C3-deficient maternal background. PMID: 18684964 Pak1 is an essential molecular target for modulating acute mast cell responses that contribute to allergic diseases. PMID: 19124833 Crry is essential for mature T-cell survival in the periphery but not for lymphogenesis in the thymus. PMID: 19136662 Crry and CD59 are critical in protecting Treg cells from complement mediated injuries. PMID: 19281793

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.