Recombinant Mouse CD8 alphaβ Heterodimer Protein

Name: Recombinant Mouse CD8 alphaβ Heterodimer Protein
Brand: Beta LifeScience
SKU: BLK-00352P-100UG
Availability: InStock

Mouse CD8 alpha&beta Heterodimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse CD8 alphaβ Heterodimer Protein is expressed from HEK293 with His tag and Flag tag at the C-terminus.It contains Lys28-Tyr196(CD8 alpha) acidic tail and Leu22-Thr175(CD8 beta) basic tail.
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Accession	P01731(CD8 alpha)&P10300(CD8 beta)
Target Symbol	CD8 alphaβ
Species	Mouse
Expression System	HEK293
Tag	C-His-Flag
Expression Range	Lys28-Tyr196(CD8 alpha) acidic tail & Leu22-Thr175(CD8 beta) basic tail
Mol. Weight	The protein has a predicted MW of 24.80 kDa (CD8 alpha) and 23.17 kDa (CD8 beta). Due to glycosylation, the protein migrates to 32-38 kDa (CD8 alpha) and 42-50 kDa (CD8 beta) based on Tris-Bis PAGE result.
Form	Lyophilized
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Endotoxin	Less than 1EU per ug by the LAL method.
Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipping	Shipped at ambient temperature.
Gene Background	CD8 alphaβ (CD8αβ) is a heterodimeric form of CD8. CD8α is required for surface expression of CD8β. The extracellular IgV-like domain of CD8α interacts with the α3 portion of the class I MHC molecule. CD8αβ is expressed on human peripheral T cells and functions as a coreceptor and can greatly increase the sensitivity and breadth of antigen recognition by CD8+ peripheral T cells bearing TCR.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.