Recombinant Mouse Ccn Family Member 1 (CCN1) Protein (His)

Name: Recombinant Mouse Ccn Family Member 1 (CCN1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-03682P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Cyr61.

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Product Overview

Description	Recombinant Mouse Ccn Family Member 1 (CCN1) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P18406
Target Symbol	CCN1
Synonyms	Ccn1; Cyr61; Igfbp10; CCN family member 1; 3CH61; Cellular communication network factor 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Protein CYR61
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-10His
Target Protein Sequence	TCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQLNEDCSKTQPCDHTKGLECNFGASSTALKGICRAQSEGRPCEYNSRIYQNGESFQPNCKHQCTCIDGAVGCIPLCPQELSLPNLGCPNPRLVKVSGQCCEEWVCDEDSIKDSLDDQDDLLGLDASEVELTRNNELIAIGKGSSLKRLPVFGTEPRVLFNPLHAHGQKCIVQTTSWSQCSKSCGTGISTRVTNDNPECRLVKETRICEVRPCGQPVYSSLKKGKKCSKTKKSPEPVRFTYAGCSSVKKYRPKYCGSCVDGRCCTPLQTRTVKMRFRCEDGEMFSKNVMMIQSCKCNYNCPHPNEASFRLYSLFNDIHKFRD
Expression Range	25-379aa
Protein Length	Full Length of Mature Protein
Mol. Weight	42.6 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3.
Subcellular Location	Secreted.
Protein Families	CCN family
Database References	KEGG: mmu:16007 STRING: 10090.ENSMUSP00000029846 UniGene: PMID: 25807183 CYR61 is upregulated in acute lung injury (ALI) and CYR61 overexpression exacerbates ALI in mice. PMID: 25713320 CYR61 silencing can blunt the malignant behavior of osteosarcoma tumor cells by limiting primary tumor growth and dissemination process. PMID: 25065593 Knock-in mice expressing a CCN1 mutant that is unable to bind alphavbeta5/alphavbeta3 were impaired in ductular reaction. CCN1, acting through its alphav beta3/alphav beta5 binding site, is critical for survival after bile duct ligation. PMID: 25822023 Findings reveal that CCN1 plays a novel role in pathogen clearance; increasing macrophage infiltration and activation resulting in increased virus clearance in tumors. PMID: 24895995 Extracellular Cyr61 convergence with growth factor signaling and integrin/FAK signaling is a new concept of growth factor-induced cell migration. PMID: 25623072 These findings implicate CCN1 as a mediator of cardioprotection induced by G-protein coupled receptor agonists that activate RhoA/MRTF-A signaling. PMID: 25106095 Our results here reveal a novel role of Cyr61 in the pathogenesis of rheumatoid arthritis PMID: 24517278 Findings suggest that that cysteine-rich 61 protein (cyr61) is expressed in airway epithelial cells and has a positive correlation with eotaxin and number of airway infiltrating eosinophils. PMID: 24142710 It provides evidence that CCN1 is released from activated platelets, suggesting that platelets constitute a novel source for CCN1 release to the circulation during sepsis. PMID: 24430538 Binding of CYR61 to LFA-1, ICAM-1 and integrin alpha6 supports the adhesion of thymic epithelial cells and T-cell. PMID: 24280864 these data reveal that de novo Cyr61 in the extracellular matrix bridges LPA and integrin pathways, which in turn, activate FAK, leading to cell migration. PMID: 24371135 CCN1 protein expression is increased in aging skeletal muscle. PMID: 24196529 The aim of the study was to evaluate CCN1 protein abundance and localization in the kidney of IL-6-deficient C57BL/6J (IL-6 KO) mice. PMID: 23690222 Degradome products of the matricellular protein CCN1 as modulators of pathological angiogenesis in the retina. PMID: 23798676 role of Cyr61 in obstructive kidney fibrosis PMID: 23457573 the potential roles of CCN1 in the inflammation and macrophage infiltration of nonalcoholic fatty liver disease ( PMID: 23071295 the matricellular protein CCN1/Cyr61 is a critical regulator of Sonic Hedgehog in pancreatic carcinogenesis PMID: 23027863 The expressions of Cyr61, MMP-2, and VEGF at the levels of mRNA and protein were decreased in the QYHJ groups. PMID: 21382954 a novel, VEGF-independent, clinically relevant, pro-angiogenic factor, CYR61, that is a transcriptional target of Hh-GLI signaling PMID: 22056874 Cyr61 expression increases with tumor grade in human osteosarcoma and demonstrate that cyr61 silencing inhibits in vitro osteosarcoma cell invasion and migration as well as in vivo lung metastases in mice. PMID: 21312266 newly identified functions of CCN1 suggest its possible therapeutic utility in ischemic retinopathy. PMID: 21212276 extracellular matrix protein CCN1 dictates TNFalpha and FasL cytotoxicity PMID: 21153366 Expression of Cyr61 is induced in fibroblasts through plasmin-induced activation of the protease-activated receptor-1 signaling pathway. PMID: 12231560 These findings establish CYR61 as a novel and essential regulator of vascular development. PMID: 12446788 Pressure overload, ischemia, & neurohormonal factors induce myocardial expression of CCN1, a potent proangiogenic factor. CCN1 may play an important role in the adaptation of the heart to cardiovascular stress. PMID: 15117851 results identify a novel and functionally important binding site for integrin alphavbeta3 and provide a new approach for dissecting alphavbeta3-specific CCN1 functions both in cultured cells and in the organism PMID: 15308622 CCN1 acts through integrin alpha6beta1 and heparan sulfate proteoglycans (HSPGs) to promote specific functions in fibroblasts. PMID: 15322081 Ccn1-null mice are impaired in cardiac valvuloseptal morphogenesis, resulting in severe atrioventricular septal defects. PMID: 17023674 Constitutively active rhFOXO3a gene transduction suppressed rmCYR61 expression in rat VSMC. Suppression of CYR61 is among several mechanisms by which FOXO3a inhibits VSMC proliferation and neointimal hyperplasia. PMID: 17234971 Intracolonic overexpression of CCN1 induced angiogenesis in mouse colon. PMID: 18599605 Caveolin-1 (cav-1)/Cyr61 interaction via integrins represents a novel pathway of Cyr61 signaling involving cav-1-dependent processes, which play a critical role in regulating hyperoxia-induced cell death PMID: 18801924 Fas-dependent hepatic apoptosis induced by an agonistic monoclonal anti-Fas antibody or intragastric administration of alcohol is severely blunted in knock-in mice expressing an apoptosis-defective Ccn1 allele. PMID: 19364818 Data suggest that signal-controlled activation of SRF, MRTF-A, and CBP provides a novel connection between mechanical stimuli and CCN1 gene expression. PMID: 19542562 The Cyr61 protein, a product of a growth factor-inducible immediate-early gene, regulates chondrogenesis in mouse limb bud mesenchymal cells. PMID: 9441684 Study shows that the interplay between CYR61-CTGF-NOV1 (CCN1) and Wnt5a in endothelial cells and pericytes determines the angiogenic outcome in a model of ischemic retinopathy. Data highlight the significance of CCN1-EC and CCN1-pericyte communication signals in driving physiological and pathological angiogenesis. PMID: 28469167 Mechanical cyclic stretch led to increased Cyr61 mRNA and protein expression, mobilized NF-kappaB from the cytoplasm to the nucleus and increased IL-8 secretion in A549 cells. PMID: 30128877 Study describes how cysteine-rich 61 (CCN1) promotes monocyte migration by upregulating monocyte chemoattractant protein-1 (CCL2) expression in osteoblasts in rheumatoid arthritis (RA) disease. CCN1 could serve as a potential target for RA treatment. PMID: 28341837 Blocking or knockdown CCN1 expression ameliorated skin inflammation and reduced the expression of CCL20 in both imiquimod and IL-23-induced psoriasis-like mouse models. PMID: 28602508 refine the leukocyte recruitment cascade model by introducing endothelium-bound CCN1 as an inflammation mediator and by demonstrating a role for platelets and patrolling Ly6C(low) monocytes in acute vascular inflammation. PMID: 27482114 This study is the first to describe how CCN1 promotes VEGF expression in osteoblasts and increased endothelial progenitor cells angiogenesis in rheumatoid arthritis disease. PMID: 27465842 Data show that cysteine rich protein 61 protein (CCN1) critically mediates doxorubicin (DOX)-induced cardiotoxicity. PMID: 27167338 Our results not only reveal a novel mechanism illustrating the role of Cyr61 in epidermis pathogenesis but also suggest that therapies targeting Cyr61 may represent a novel strategy in the treatment of psoriasis vulgaris. PMID: 27856305 The LPA1-CCN1 axis may be the central control for SMC migration. PMID: 27760754 these data suggest that the WNT/beta-catenin signaling pathway enhances pancreatic cancer development and malignancy in part via up-regulation of CYR61. PMID: 27889647 Lysophosphatidic acid induced time-dependent phosphorylation of serum response factor and CRE-binding protein in in smooth muscle cells, inducing transcription of CCN1. PMID: 28157379 Data show that Cysteine-rich 61 (CYR61) acts as a tumor-promoting gene in pancreatic neuroendocrine tumors. PMID: 26625209 CCN1 is an important regulator of chondrocyte maturation during cartilage development and homeostasis. PMID: 26363286 CCN1 exacerbates tracheal obliterative bronchiolitis by enhancing fibroproliferation via an alphavbeta3-integrin-independent pathway. PMID: 26174800 Data show that RNA silencing of CCN family member 1 protein (CCN1) inhibits retinal neovascularization (RNV) by inhibiting phosphoinositide 3-kinase (PI3K)/AKT protein signaling in mouse pup model of oxygen-induced retinopathy (OIR). PMID: 26459773

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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