Recombinant Mouse Calcitonin Gene-Related Peptide 1 (CALCA) Protein (His-SUMO)

Beta LifeScience SKU/CAT #: BLC-06083P

Recombinant Mouse Calcitonin Gene-Related Peptide 1 (CALCA) Protein (His-SUMO)

Beta LifeScience SKU/CAT #: BLC-06083P
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Product Overview

Description Recombinant Mouse Calcitonin Gene-Related Peptide 1 (CALCA) Protein (His-SUMO) is produced by our E.coli expression system. This is a protein fragment.
Purity Greater than 90% as determined by SDS-PAGE.
Uniprotkb Q99JA0
Target Symbol CALCA
Species Mus musculus (Mouse)
Expression System E.coli
Tag N-6His-SUMO
Target Protein Sequence SCNTATCVTHRLAGLLSRSGGVVKDNFVPTNVGSEAF
Expression Range 83-119aa
Protein Length Partial
Mol. Weight 16.8 kDa
Research Area Neuroscience
Form Liquid or Lyophilized powder
Buffer Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.
Subcellular Location Secreted.
Protein Families Calcitonin family
Database References

KEGG: mmu:12310

UniGene: PMID: 29913443

  • alphaCGRP overexpression causes an increase in thermal reaction and downregulation of the cardiovascular system, presumably due in increased levels of alphaCGRP. PMID: 30068853
  • Glucagon-like peptide (GLP)-1 modulates epithelial ion transport indirectly by activating calcitonin gene-related peptide (CGRP)-containing submucosal enteric neurons in the mouse colon. This GLP-1-CGRP response was area-specific and could potentially contribute to the diarrheal side effect of certain GLP-1 receptor therapeutics. PMID: 28695626
  • This study demonstrated that the expression patterns of the calcitonin-related polypeptide alpha and tyrosine hydroxylase formed opposing gradients, with tyrosine hydroxylase being preferentially expressed in apical and calcitonin-related polypeptide alpha in basal type II afferent neurons, indicating heterogeneity among type II afferent neurons. PMID: 29055051
  • CGRP-1 is essential for pain-associated plasticity in the central amygdala. PMID: 28833700
  • These results suggest that neuronal TRPV1 signaling in periodontal tissue is crucial for the regulation of osteoclastogenesis via the neuropeptide CGRP. PMID: 27388773
  • alphaCGRP played a synergic role in angiogenesis and osseointegration, partly as a consequence of its vasodilative activity. PMID: 29421662
  • results indicate that CGRP regulates macrophage polarization and inhibits inflammation in murine macrophages. PMID: 28892747
  • The results indicate a central role of neuroendocrine peptide aminoprocalcitonin (NPCT) in Alzheimer disease pathogenesis and suggest NPCT as a potential biomarker and therapeutic target. PMID: 27497681
  • In addition to its actions in the cardiovascular system, endogenous CGRP is a key regulator of metabolism and energy homeostasis in vivo. PMID: 28324021
  • findings establish CGRP neurons in the parabrachial nucleus as key mediators of cancer-induced appetite suppression and associated behavioral changes. PMID: 28581479
  • These results identify an important, possibly dual role of CGRP in Amyotrophic lateral sclerosis pathogenesis. PMID: 27554772
  • functional inactivation of CGRP neurons markedly increases meal size, with meal frequency being reduced in a compensatory manner, and renders mice insensitive to the anorexic effects of meal-related satiety peptides. PMID: 27166945
  • Mouse and human heart valves expressed mRNAs for the CRL ligands adrenomedullin (AM), adrenomedullin-2 (AM-2) and calcitonin gene-related peptide (CGRP) and for their receptor components, i.e., CRL and receptor-activity-modifying proteins 1-3. PMID: 27553639
  • The abundance of mRNA of CGRP and bone matrix markers (Col I, Col II, and OPN) was low at P5 in the tibia. These differences in CGRP and other mRNAs may induce a different manner of ossification between the mandible and tibia. Therefore, a time lag of ossification occurs between the mandible and tibia during foetal development. PMID: 28348418
  • Suggest that cochlear function is enhanced by maturation of CGRP receptor complexes. PMID: 27440744
  • the generation of Tregs may be promoted by naive CD4+ T cell: neuron interaction through the release of neuropeptide CGRP. PMID: 27022966
  • The observations of this study imply that Adelta afferents expressing CGRP-IR transduce information from different sites within the skin. PMID: 26010480
  • SHH may affect alveolar bone healing by interacting with CGRP-positive sensory neurons and thus regulate the socket's healing process after tooth extraction. PMID: 26427874
  • TRPM8 signaling in mucosal sensory neurons is indispensable for the regulation of innate inflammatory responses via the neuropeptide CGRP. PMID: 25269705
  • CGRP suppresses the development of leaky choroidal neovascularization through negative regulation of inflammation. PMID: 25857228
  • CT has a role in skeletal biology and S1P acts as an osteoanabolic molecule and has a role in crosstalk between osteoclasts and osteoblasts PMID: 25333900
  • CGRP is an inhibitory transmitter that shapes peripheral taste signals via serotonergic signaling during processing gustatory information in taste buds. PMID: 26377461
  • The increased responsiveness to intraplantar calcitonin gene-related peptide suggests that the peripheral actions of calcitonin gene-related peptide are enhanced as a result of the neurofibromin deficit. PMID: 25184332
  • This study provides direct ultrastructural evidences for the synaptic contacts between CGRP-positive terminals and GABAergic neurons within the central nucleus of amygdala. PMID: 24794145
  • activation of periosteal mineralizing surfaces in response to mechanical loading of bone is CGRPalpha-dependent in vivo. PMID: 25536054
  • Sensory neuron-derived CGRP sustains mechanical hypersensitivity and spinal microglial reactivity in collagen-induced arthritis, suggesting that central mechanisms play critical roles in chronic inflammatory pain. PMID: 25707377
  • The parabrachial CGRP neurons mediate a gastrointestinal distress signal required to establish conditioned taste aversion. PMID: 25788675
  • findings uncover a context-dependent molecular mechanism of TRPV1 algesic sensitization and a previously unrecognized role of alphaCGRP in LDCV mobilization in peptidergic nociceptors PMID: 25489075
  • The results of this study indicated that the enhanced behavioral responses to cold stimuli in CGRPalpha sensory neuron-ablated mice are dependent on functional TRPM8. PMID: 25406633
  • demonstrated that simultaneous downregulation of PPARgamma and upregulation of CGRP was efficient in suppressing adipogenic differentiation of BMSCs and promoting their osteogenic differentiation PMID: 24633961
  • communication through CGRP as a novel costimulatory pathway promoting the development of a regulatory phenotype of TLR4-stimulated macrophages. PMID: 25316186
  • neuropeptides CGRP and VIP have an important role in suppressing bone resorptive activities through RANKL/OPG pathway, similar to mechanical loading. PMID: 24717410
  • These results suggest that contribution of the alphaCGRP system depends on the modality of pain and the stage of inflammation PMID: 24992521
  • This study showed that the neurotransmitter CGRP plays a key role in ensuring VOR efficacy. PMID: 25080603
  • CGRP regulates Th1 type reactions. PMID: 24466057
  • There is an ongoing role of alpha-calcitonin gene-related peptide as part of a protective network against hypertension, vascular hypertrophy, and oxidative stress. PMID: 24516108
  • The activation of alphaCGRP may induce other neuropeptides associated with immunomodulation at central nervous system. level PMID: 24021706
  • Alpha-CGRP plays a significant protective role in heart failure which may be mediated by decreased inflammation, cell death, and fibrosis. PMID: 23816470
  • SP-, CGRP-, and VGLUT2-mediated transmission together were found to play a role in the development of mechanical hyperalgesia after persistent inflammation. PMID: 24275230
  • Alpha-CGRP may play an important role in the regulation of kainic acid-induced pyramidal cell death in CA3 region of the hippocampus. PMID: 23587546
  • Data indicate that transient receptor potential ion channels and neuropeptides substance P and calcitonin gene-related peptide have important roles in formaldehyde promoted-asthma. PMID: 23671638
  • The results clearly demonstrate that endogenous CGRP suppresses the oxidative stress and VSMC proliferation induced by vascular injury. PMID: 23416515
  • DNA methylation changes were noted in the Calca gene during chronic cystitis in a murine model. PMID: 23806407
  • Deletion of the gene encoding calcitonin and calcitonin gene-related peptide alpha did not influence the course of sepsis in three separate mouse models of infection. PMID: 23526213
  • CT/alpha-CGRP modulates bone cell activity in PIO in aged mice in a way that is distinct from young animals PMID: 23357366
  • CGRP-immunoreactive nerve fibers were specifically increased with the development of food allergy. PMID: 23261435
  • The CGRP/cyclic AMP/protein kinase A (PKA) pathway is important in regulating the differentiation of T helper (Th)9 cell subset. PMID: 23509367
  • The data of this study revealed that CGRPalpha sensory neurons encode heat and itch and tonically cross-inhibit cold-responsive spinal neurons. PMID: 23523592
  • Hydrogen sulfide-evoked release of CGRP from sensory nerves induces increases in microcirculation of mouse ear following activation of TRPA1 receptors. PMID: 22721614

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    Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

    Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

    Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

    Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

    To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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