Recombinant Mouse Calcitonin Gene-Related Peptide 1 (CALCA) Protein (His-SUMO)

Name: Recombinant Mouse Calcitonin Gene-Related Peptide 1 (CALCA) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-06083P
Price: 549.6 USD
Availability: InStock

Collections: Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Cytokines, Cytokines and growth factors, Growth factors and receptors, Recombinant proteins

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Product Overview

Description	Recombinant Mouse Calcitonin Gene-Related Peptide 1 (CALCA) Protein (His-SUMO) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q99JA0
Target Symbol	CALCA
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	SCNTATCVTHRLAGLLSRSGGVVKDNFVPTNVGSEAF
Expression Range	83-119aa
Protein Length	Partial
Mol. Weight	16.8 kDa
Research Area	Neuroscience
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.
Subcellular Location	Secreted.
Protein Families	Calcitonin family
Database References	KEGG: mmu:12310 UniGene: Mm.4361
Tissue Specificity	Detected in nerve cells of cerebrum, hippocampus and pons/midbrain in newborns, and only in nerve cells of pons/midbrain in adult.

Gene Functions References

Hepatic PCT expression is upregulated in ALF. Hepatic macrophages but not hepatocytes are the cell source of hepatic PCT expression. PMID: 29913443
alphaCGRP overexpression causes an increase in thermal reaction and downregulation of the cardiovascular system, presumably due in increased levels of alphaCGRP. PMID: 30068853
Glucagon-like peptide (GLP)-1 modulates epithelial ion transport indirectly by activating calcitonin gene-related peptide (CGRP)-containing submucosal enteric neurons in the mouse colon. This GLP-1-CGRP response was area-specific and could potentially contribute to the diarrheal side effect of certain GLP-1 receptor therapeutics. PMID: 28695626
This study demonstrated that the expression patterns of the calcitonin-related polypeptide alpha and tyrosine hydroxylase formed opposing gradients, with tyrosine hydroxylase being preferentially expressed in apical and calcitonin-related polypeptide alpha in basal type II afferent neurons, indicating heterogeneity among type II afferent neurons. PMID: 29055051
CGRP-1 is essential for pain-associated plasticity in the central amygdala. PMID: 28833700
These results suggest that neuronal TRPV1 signaling in periodontal tissue is crucial for the regulation of osteoclastogenesis via the neuropeptide CGRP. PMID: 27388773
alphaCGRP played a synergic role in angiogenesis and osseointegration, partly as a consequence of its vasodilative activity. PMID: 29421662
results indicate that CGRP regulates macrophage polarization and inhibits inflammation in murine macrophages. PMID: 28892747
The results indicate a central role of neuroendocrine peptide aminoprocalcitonin (NPCT) in Alzheimer disease pathogenesis and suggest NPCT as a potential biomarker and therapeutic target. PMID: 27497681
In addition to its actions in the cardiovascular system, endogenous CGRP is a key regulator of metabolism and energy homeostasis in vivo. PMID: 28324021
findings establish CGRP neurons in the parabrachial nucleus as key mediators of cancer-induced appetite suppression and associated behavioral changes. PMID: 28581479
These results identify an important, possibly dual role of CGRP in Amyotrophic lateral sclerosis pathogenesis. PMID: 27554772
functional inactivation of CGRP neurons markedly increases meal size, with meal frequency being reduced in a compensatory manner, and renders mice insensitive to the anorexic effects of meal-related satiety peptides. PMID: 27166945
Mouse and human heart valves expressed mRNAs for the CRL ligands adrenomedullin (AM), adrenomedullin-2 (AM-2) and calcitonin gene-related peptide (CGRP) and for their receptor components, i.e., CRL and receptor-activity-modifying proteins 1-3. PMID: 27553639
The abundance of mRNA of CGRP and bone matrix markers (Col I, Col II, and OPN) was low at P5 in the tibia. These differences in CGRP and other mRNAs may induce a different manner of ossification between the mandible and tibia. Therefore, a time lag of ossification occurs between the mandible and tibia during foetal development. PMID: 28348418
Suggest that cochlear function is enhanced by maturation of CGRP receptor complexes. PMID: 27440744
the generation of Tregs may be promoted by naive CD4+ T cell: neuron interaction through the release of neuropeptide CGRP. PMID: 27022966
The observations of this study imply that Adelta afferents expressing CGRP-IR transduce information from different sites within the skin. PMID: 26010480
SHH may affect alveolar bone healing by interacting with CGRP-positive sensory neurons and thus regulate the socket's healing process after tooth extraction. PMID: 26427874
TRPM8 signaling in mucosal sensory neurons is indispensable for the regulation of innate inflammatory responses via the neuropeptide CGRP. PMID: 25269705
CGRP suppresses the development of leaky choroidal neovascularization through negative regulation of inflammation. PMID: 25857228
CT has a role in skeletal biology and S1P acts as an osteoanabolic molecule and has a role in crosstalk between osteoclasts and osteoblasts PMID: 25333900
CGRP is an inhibitory transmitter that shapes peripheral taste signals via serotonergic signaling during processing gustatory information in taste buds. PMID: 26377461
The increased responsiveness to intraplantar calcitonin gene-related peptide suggests that the peripheral actions of calcitonin gene-related peptide are enhanced as a result of the neurofibromin deficit. PMID: 25184332
This study provides direct ultrastructural evidences for the synaptic contacts between CGRP-positive terminals and GABAergic neurons within the central nucleus of amygdala. PMID: 24794145
activation of periosteal mineralizing surfaces in response to mechanical loading of bone is CGRPalpha-dependent in vivo. PMID: 25536054
Sensory neuron-derived CGRP sustains mechanical hypersensitivity and spinal microglial reactivity in collagen-induced arthritis, suggesting that central mechanisms play critical roles in chronic inflammatory pain. PMID: 25707377
The parabrachial CGRP neurons mediate a gastrointestinal distress signal required to establish conditioned taste aversion. PMID: 25788675
findings uncover a context-dependent molecular mechanism of TRPV1 algesic sensitization and a previously unrecognized role of alphaCGRP in LDCV mobilization in peptidergic nociceptors PMID: 25489075
The results of this study indicated that the enhanced behavioral responses to cold stimuli in CGRPalpha sensory neuron-ablated mice are dependent on functional TRPM8. PMID: 25406633
demonstrated that simultaneous downregulation of PPARgamma and upregulation of CGRP was efficient in suppressing adipogenic differentiation of BMSCs and promoting their osteogenic differentiation PMID: 24633961
communication through CGRP as a novel costimulatory pathway promoting the development of a regulatory phenotype of TLR4-stimulated macrophages. PMID: 25316186
neuropeptides CGRP and VIP have an important role in suppressing bone resorptive activities through RANKL/OPG pathway, similar to mechanical loading. PMID: 24717410
These results suggest that contribution of the alphaCGRP system depends on the modality of pain and the stage of inflammation PMID: 24992521
This study showed that the neurotransmitter CGRP plays a key role in ensuring VOR efficacy. PMID: 25080603
CGRP regulates Th1 type reactions. PMID: 24466057
There is an ongoing role of alpha-calcitonin gene-related peptide as part of a protective network against hypertension, vascular hypertrophy, and oxidative stress. PMID: 24516108
The activation of alphaCGRP may induce other neuropeptides associated with immunomodulation at central nervous system. level PMID: 24021706
Alpha-CGRP plays a significant protective role in heart failure which may be mediated by decreased inflammation, cell death, and fibrosis. PMID: 23816470
SP-, CGRP-, and VGLUT2-mediated transmission together were found to play a role in the development of mechanical hyperalgesia after persistent inflammation. PMID: 24275230
Alpha-CGRP may play an important role in the regulation of kainic acid-induced pyramidal cell death in CA3 region of the hippocampus. PMID: 23587546
Data indicate that transient receptor potential ion channels and neuropeptides substance P and calcitonin gene-related peptide have important roles in formaldehyde promoted-asthma. PMID: 23671638
The results clearly demonstrate that endogenous CGRP suppresses the oxidative stress and VSMC proliferation induced by vascular injury. PMID: 23416515
DNA methylation changes were noted in the Calca gene during chronic cystitis in a murine model. PMID: 23806407
Deletion of the gene encoding calcitonin and calcitonin gene-related peptide alpha did not influence the course of sepsis in three separate mouse models of infection. PMID: 23526213
CT/alpha-CGRP modulates bone cell activity in PIO in aged mice in a way that is distinct from young animals PMID: 23357366
CGRP-immunoreactive nerve fibers were specifically increased with the development of food allergy. PMID: 23261435
The CGRP/cyclic AMP/protein kinase A (PKA) pathway is important in regulating the differentiation of T helper (Th)9 cell subset. PMID: 23509367
The data of this study revealed that CGRPalpha sensory neurons encode heat and itch and tonically cross-inhibit cold-responsive spinal neurons. PMID: 23523592
Hydrogen sulfide-evoked release of CGRP from sensory nerves induces increases in microcirculation of mouse ear following activation of TRPA1 receptors. PMID: 22721614

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Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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