Recombinant Mouse C-X-C Motif Chemokine 9 Protein (CXCL9), Active

Name: Recombinant Mouse C-X-C Motif Chemokine 9 Protein (CXCL9), Active
Brand: Beta LifeScience
SKU: BLC-05489P
Availability: InStock

Collections: All products, Buy cytokines, chemokines, and growth factors for research online, Chemokines and receptors – essential regulators of immune response, High-quality cytokines for advanced research, High-quality recombinant proteins

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Product Overview

Description	Recombinant Mouse C-X-C Motif Chemokine 9 Protein (CXCL9), Active is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 95% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 0.1-1.0 ng/ml.
Uniprotkb	P18340
Target Symbol	CXCL9
Synonyms	Cxcl9; Mig; Scyb9C-X-C motif chemokine 9; Gamma-interferon-induced monokine; Monokine induced by interferon-gamma; MIG; MuMIG; Protein m119; Small-inducible cytokine B9
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	Tag-Free
Complete Sequence	TLVIRNARCS CISTSRGTIH YKSLKDLKQF APSPNCNKTE IIATLKNGDQ TCLDPDSANV KKLMKEWEKK INQKKKQKRG KKHQKNMKNR KPKTPQSRRR SRKTT
Expression Range	22-126
Protein Length	Partial of M34815
Mol. Weight	12.2 kDa
Research Area	Immunology
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	May be a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response.
Subcellular Location	Secreted.
Protein Families	Intercrine alpha (chemokine CxC) family
Database References	KEGG: mmu:17329 STRING: 10090.ENSMUSP00000108716 UniGene: PMID: 29712506 Cxcl9 has a role in angiogenesis and osteogenesis in bone. PMID: 27966526 Data suggest that the CXCL9-CXCR3 axis plays a pivotal role in the liver-specific distribution of TRAIL+ NK cells in mice. PMID: 29088306 IFNalphaR1 signaling promoted CXCL9 and CXCL10 synthesis, suggesting that these chemokines might be involved in the LPS and CD134 costimulation response. PMID: 28432083 CXCL10 plays in the pathogenesis of recurrent Herpetic stromal keratitis, and that CXCL9 displays its importance when CXCL10 is absent. PMID: 28282568 CXCL9 expression is strongly upregulated in PGRN KO mice and its level is correlated with severity of inflammation in a dermatitis model. PMID: 26892362 hepatic expression of the inflammatory CXC chemokine ligands (CXCL)9 and CXCL10 strongly increased whereas homeostatic CXCL12 significantly decreased. PMID: 26052942 Here, we report the evidence for the production of MIG, a second CXCR3 ligand, during the primary immune response to HSV-1 corneal infection. PMID: 25207638 These findings identify a novel role for the immune cell-derived CXCL9 chemokine in directing a protective antimicrobial response in the intestinal mucosa. PMID: 25643352 These results indicate that CXCL9 is crucial for recruiting immune T cells into the brain and inducing an accumulation of the T cells into the areas where tachyzoites proliferate to prevent reactivation of chronic T. gondii infection. PMID: 25432064 tumours are characterized by expression of inflammatory chemokines (CCL2, CCL5, CCL7, CCL8, CCL12, CXCL9, CXCL10 and CX3CL1), reflected by an enrichment of activated Foxp3(-) and Foxp3(+) T cells PMID: 25495686 Aged mice had similar levels of IL-1beta, TNF, IFN-gamma, IL-17, and granulocyte colony-stimulating factor following S. pneumoniae infection, compared with young mice, but increased levels of the chemokines CXCL9, CXCL12, CCL3, CCL4, CCL5, CCL11, and CCL17. PMID: 25595646 Data indicate that a feed-forward CXCL9-dependent circuit provided additional chemotactic cues that further increase local memory cell density. PMID: 23352234 IFN-gamma-mediated loss of Mig expression in cutaneous tumors as a potent mechanism of immunoediting that results in increased tumor resistance to T cell-mediated immunity. PMID: 23241877 CXCL9/10 have antifibrotic roles on liver non-parenchymal cells PMID: 22905138 findings show that effector T cells cannot accumulate within the decidua, the specialized stromal tissue encapsulating the fetus and placenta; impaired accumulation was in part attributable to the epigenetic silencing of key T cell-attracting inflammatory chemokine genes in decidual stromal cells PMID: 22679098 Mig contributes to the acute lethal toxicity arising from 5-FU administration. PMID: 22474250 Cxcr3, Cxcl9 and Cxcl10 are increased in alopecia areata. PMID: 22358057 The results identify direct angiostatic and antifibrotic effects of the Cxcr3 ligand Cxcl9 in a model of experimental liver fibrosis. PMID: 22237831 MIG/CXCL9 is expressed in the lungs upon pneumococcal infection in a MyD88-dependent manner PMID: 20381636 Expression of the chemokine Mig (CXCL9) was increased 2.8-fold in tumors from Egr-1 knockout mice. PMID: 19200397 CXCL9 promotes the development of IFN-gamma-producing CD8 T cells, and CXCL10 antagonizes this skewing during allograft rejection. PMID: 20194716 CCL2, CXCL9 and CXCL2 mRNA are up-regulated after oral Salmonella infection in Peyer's patches and lymph nodes coincident with the first arrival of monocytes and neutrophils PMID: 19839009 MIG (CXCL9) chemokine gene therapy combines with antibody-cytokine fusion protein to suppress growth and dissemination of murine colon carcinoma. PMID: 11731434 in study of relevance of chemokine expression to selective migration of t-cells and the disease localization in murine graft-versus-host disease, Mig was found to be predominantly expressed in spleen, liver, and not skin, and not heart. PMID: 12098066 NF-kappaB has a critical role in mediating IFN-gamma-induced MIG (monokine induced by IFN-gamma) expression independent of hyaluronan PMID: 12226082 involved in T cell cardiac allograft vasculopathy PMID: 12368204 Data show that the transcriptional coactivator CREB-binding protein (CBP) mediated the STAT1/NF-kappaB synergy for transcription of the gene for CXC ligand 9 an interferon-gamma (IFN-gamma)-inducible chemokine. PMID: 12403783 full potency of SLC/CCL21-mediated anti-tumor responses require in part the induction of IFNgamma, MIG/CXCL9 and IP-10/CXCL10 PMID: 12740040 Peak of expression of CXCL9 and CXCL10 occurred 4 days before CD8+ T cells infiltrated infected tissues. CXCL9 and CXCL10 may play role early during immune response against rickettsial infections. PMID: 14507644 Mig functions as a negative regulator of murine eosinophils PMID: 14769916 exogenous CXCL9 stimulated CD4 lymphocyte proliferation in a MHC class II-mismatched MLR and increased the number of IFN-gamma-producing CD4 lymphocytes PMID: 15187119 Interactions involving CXCR3 and its primary ligands Mig and IP-10 significantly contribute to donor T cell recruitment to the lung after allogeneic stem cell transplantation. PMID: 15265940 RANKL stimulates the serine phosphorylation of STAT1, causing MIG gene transcription and secretion, which may have a role in recruiting CXCR3-positive osteoclast precursors and osteoclasts to bone remodeling or inflammatory sites. PMID: 15585657 IFN-gamma knockout mice, which manifested depressed ear-swelling following delayed hypersensitsivity challenge, made no Mig. PMID: 15629884 Results suggest that in the sensitized host, CXCR3, IP-10, and Mig are required for optimal delayed hypersensitivity responsiveness but are not essential for containing HSV-1 replication. PMID: 15708587 results suggest that MUM1 plays roles in the progression of B-cell lymphoma/leukemia by regulating the expression of various genes including MIG. PMID: 15959530 CXCL9 has a role in graft rejection in the absence of CCL19 and CCL21 PMID: 16095489 MIG mRNA expression in the lungs of Klebsiella-infected mice requires the endogenous production of IFN-gamma. PMID: 16299319 CXCL9 signaling enhances immune responses following Trypanosoma cruzi infection; transcripts for CXCL9 remain elevated during chronic infection PMID: 16368965 CXCL9 is up-regulated in unique patterns following tracheal transplantation in mice. Deletion of CXCL9 does not affect airway obliteration. PMID: 16709871 These results suggest that the more aggressive rejection of xenografts compared with allografts is due to the earlier expression of CXC-chemokines, IP-10 and MIG, and subsequent adjuvant effects of proinflammatory cytokines. PMID: 16768726 Collectively, the results suggest a non-redundant role for CXCL9 and CXCL10 in response to ocular HSV-1 infection in terms of controlling virus replication and recruitment of CD4(+) T cells into the cornea. PMID: 17296171 Acute ethanol intoxication impairs lung expression of Cxcl9, interfering with pulmonary response to bacterial challenge. PMID: 17889309 IFN-gamma is mediator of Cxcl10 and Cxcl9 gene expression in experimental autoimmune encephalomyelitis(EAE). It differentially regulates expression of these genes by astrocytes and microglia. Differential glial localization of these chemokines in EAE. PMID: 17902170 The absence of CXCL9 or CXCL10 expression significantly alters the ability of the host to control genital HSV-2 infection through the mobilization of effector cells to sites of infection. PMID: 18178850 These data demonstrate that CXCR3 on CD8(+) T cells is required for T cell recruitment into the brain and the development of murine cerebral malaria. PMID: 18347328 Liver sinusoidal endothelial cells present chemokines (CXCL12 and CXCL9) to circulating lymphocytes. PMID: 18697212 CXCR3 ligands, IP10 and MIG, contribute to Th1-induced inflammation but not to homing of Th1 cells into the lung. PMID: 18716926 CXCL9 promotes protection from coronavirus-induced neurological and liver disease. PMID: 18973912

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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