Recombinant Mouse Beta-1 Adrenergic Receptor (ADRB1) Protein (His-SUMO)

Beta LifeScience SKU/CAT #: BLC-07924P
Greater than 85% as determined by SDS-PAGE.
Greater than 85% as determined by SDS-PAGE.

Recombinant Mouse Beta-1 Adrenergic Receptor (ADRB1) Protein (His-SUMO)

Beta LifeScience SKU/CAT #: BLC-07924P
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Product Overview

Description Recombinant Mouse Beta-1 Adrenergic Receptor (ADRB1) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity Greater than 85% as determined by SDS-PAGE.
Uniprotkb P34971
Target Symbol ADRB1
Synonyms (Beta-1 adrenoreceptor)(Beta-1 adrenoceptor)
Species Mus musculus (Mouse)
Expression System in vitro E.coli expression system
Tag N-6His-SUMO
Target Protein Sequence MGAGALALGASEPCNLSSAAPLPDGAATAARLLVLASPPASLLPPASEGSAPLSQQWTAGMGLLLALIVLLIVVGNVLVIVAIAKTPRLQTLTNLFIMSLASADLVMGLLVVPFGATIVVWGRWEYGSFFCELWTSVDVLCVTASIETLCVIALDRYLAITSPFRYQSLLTRARARALVCTVWAISALVSFLPILMHWWRAESDEARRCYNDPKCCDFVTNRAYAIASSVVSFYVPLCIMAFVYLRVFREAQKQVKKIDSCERRFLGGPARPPSPEPSPSPGPPRPADSLANGRSSKRRPSRLVALREQKALKTLGIIMGVFTLCWLPFFLANVVKAFHRDLVPDRLFVFFNWLGYANSAFNPIIYCRSPDFRKAFQRLLCCARRAACRRRAAHGDRPRASGCLARAGPPPSPGAPSDDDDDDAGTTPPARLLEPWTGCNGGTTTVDSDSSLDEPGRQGFSSESKV
Expression Range 1-466aa
Protein Length Full Length
Mol. Weight 63.4 kDa
Research Area Others
Form Liquid or Lyophilized powder
Buffer Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Mediates Ras activation through G(s)-alpha- and cAMP-mediated signaling. Involved in the regulation of sleep/wake behaviors.
Subcellular Location Cell membrane; Multi-pass membrane protein. Early endosome.
Protein Families G-protein coupled receptor 1 family, Adrenergic receptor subfamily, ADRB1 sub-subfamily
Database References
Tissue Specificity In brain, expressed by glutamatergic and GABAergic neurons of the dorsal pons (at protein level).

Gene Functions References

  1. Cardiac specific expression of the mutated Cav1.2PKA_P2(-/-) gene reduces Cav1.2 alpha1c protein concentration, calcium current (ICa), and the beta-adrenergic stimulation of L-type ICa in cardiomyocytes. PMID: 28778765
  2. Proliferation in cardiac fibroblasts induced by beta1-adrenoceptor autoantibody results in endomyocardial fibrosis. PMID: 27577254
  3. the present study demonstrated that cardiac contractility/relaxation and heart rate is increased in beta1AR TG and beta2AR TG mice, and indicated that this increase may be related to the overexpression of G proteins and Gproteinassociated proteins. PMID: 28487987
  4. ghrelin has a critical role in preventing hypoglycemia and promoting survival during severe caloric restriction, a process that requires cell-expressed beta1AR PMID: 27548523
  5. knocking out of the beta1/2 receptor significantly diminished the ST25 acupuncture-induced inhibition of gastric motility and jejunal motility without significantly altering the enhancement of colonic motility induced by acupuncture at ST25. PMID: 27978539
  6. Computer simulations model the responses to moderate stimulation/inhibition of the beta1-adrenergic signaling system and its components in mouse ventricular myocytes. PMID: 26936457
  7. Our findings that BAG3 is localized at the sarcolemma and t-tubules while modulating myocyte contraction and action potential duration through specific interaction with the beta1-adrenergic receptor and L-type Ca(2+) channel provide novel insight into the role of BAG3 in cardiomyopathies and increased arrhythmia risks in heart failure. PMID: 26796036
  8. Galphai2 deficiency combined with cardiac beta1-adrenoceptor overexpression strongly impaired survival and cardiac function, leading to dilated cardiomyopathy. PMID: 26464333
  9. Knockout of the C5aR1 attenuated the effect of beta1-AR in the heart, suggesting an association between the beta1-AR and C5aR1, although further investigation is required to determine if this is a direct or causal association PMID: 26727203
  10. The beta1-AR may play an important role in the development of atrial fibrillation. PMID: 26203906
  11. Partial gap junction uncoupling increased propensity and amplitude of Ca(2+) alternans, and made them more sensitive to reversal by beta-AR activation, as in isolated myocytes. PMID: 25828762
  12. These data reveal a novel interplay between the E2F pathway, beta2-adrenergic/PKA/PDE4D, and ERK/c-Src axis in fine tuning the pathological hypertrophic growth response. PMID: 25944088
  13. Myocardial adrenergic receptor beta 1 preferentially associates with AC5. PMID: 25677623
  14. role of beta-ARs in early muscle regeneration PMID: 25000590
  15. Phosphorylation of beta1-AR by PKA stimulates active Ca(2+)influx through TRPV5. PMID: 24828496
  16. Sustained beta1-adrenoceptor activation in the diseased heart exacerbates left ventricular remodelling and therefore may promote disease progression from compensatory hypertrophy to heart failure PMID: 23750586
  17. This study report that both beta1- and beta2-ARs are expressed in a majority of GABAergic interneurons in the medial prefrontal cortex of mice. PMID: 24215978
  18. Calcium {Ca(2+)} entry through beta-adrenergic-stimulated LTCC underlies hypoxia-induced [Ca(2+)]i overload and the ensuing loss of mitochondrial function in intact heart. PMID: 24041537
  19. Data indicate that ischemic injury coupled to G protein-coupled receptor beta1/2 adrenergic receptors activation required GRK2 (GPCR kinase 2) activation. PMID: 24170934
  20. Our findings indicate a novel function for beta1AR-mediated beta-arrestin1 signaling activated by carvedilol in miR biogenesis. PMID: 24334028
  21. a significant role for the AKAP5 scaffold in signaling and trafficking of the beta1-AR in cardiac myocytes and mammalian cells. PMID: 24121510
  22. beta1-adrenergic receptor recycles via a membranous organelle, recycling endosome, by binding with sorting nexin27. PMID: 23780416
  23. We demonstrate that restoration of cardiac plasma membrane levels of S1PR1 produces beneficial effects that counterbalance the deleterious beta1AR overstimulation in heart failure. PMID: 23969695
  24. Canonical betaAR-mediated signaling coupled to PKA activation results in phosphorylation of spinophilin, disrupting its interaction with alpha2AARs and accelerating alpha2AAR endocytic responses. PMID: 23965992
  25. CREB-binding protein (CBP) regulates beta-adrenoceptor (beta-AR)-mediated apoptosis. PMID: 23579242
  26. TNFalpha-induced betaAR desensitization is mediated by GRK2 and is independent of Gbetagamma, uncovering a hitherto unknown cross-talk between TNFalpha and betaAR function, providing the underpinnings of inflammation-mediated cardiac dysfunction. PMID: 23785004
  27. Adrb1 activates Epac2 to induce sarcoplasmic reticulum calcium leakage via a pathway that contributes to arrhythmia and reduced cardiac function. PMID: 23363625
  28. In muscararinic M2 receptor knockout mice,heart function is not much affected; as possible mechanisms of this normal cardiac function, decreases of gene expression and receptor binding of beta1- and beta2-adrenoceptors should be considered PMID: 23093370
  29. A potential cooperative role for beta(1) and beta(2) adrenergic receptors in stress-induced reinstatement. PMID: 22593095
  30. The beta(1) signaling pathway mediates most of the sympathetic nervous system stimulation of adaptive thermogenesis. PMID: 22728333
  31. beta1- and beta2-adrenergic signaling exert opposite effects on bone- beta1 exerting a predominant anabolic stimulus in response to mechanical stimulation and during growth, whereas beta2-adrenergic receptor signaling mainly regulates bone resorption during aging. PMID: 22407956
  32. Data show that overexpression of beta1- and beta2-adrenergic receptors in hepatocytes from young mice and rats increased cellular lipid content. PMID: 22457517
  33. Norepinephrine regulates intestinal mucosal immune responses mediated by intestinal intraepithelial lymphocytes via beta adrenoreceptors. PMID: 22398028
  34. Induction of Fos by ssadrenergic signaling is critical for the full activation of CA1 pyramidal neurons in response to excitatory input from CA3 pyramidal neurons conveying retrieved contextual information. PMID: 21377513
  35. beta-Adrenergic receptor-PI3K signaling crosstalk in mouse heart PMID: 22028912
  36. Confinement of beta 1- and beta 2-adrenergic receptors in the plasma membrane of cardiomyocytes is mediated by selective interactions with PDZ domain and A-kinase anchoring proteins PMID: 21680711
  37. High (stress) levels of epinephrine directly compromise the activity of neonatal mouse skin fibroblasts through the activation of beta1-, beta2- and beta3-adrenoceptors, but not through alpha1- and alpha2-adrenoceptors. PMID: 21366703
  38. renin expression was found to be significantly lower at all developmental stages in the kidneys of adrenergic beta(1)/beta(2) knockout mice PMID: 21389089
  39. b1-adrenoceptors are the predominant b-adrenoceptor in mouse astrocytes and are primarily responsible for cAMP production in response to b-adrenoceptor stimulation PMID: 21138422
  40. negative inotropy and heart hypertrophy occur in total adrenergic beta receptor deficiency PMID: 20406048
  41. Results suggest that catecholamines induce myocyte necrosis primarily through beta(1)-AR-mediated increases in L-type calcium channel activity, but other mechanisms are also involved in rodents. PMID: 20495143
  42. the unique signaling effects of enhanced beta(2)AR and AC5, which have the potential to afford benefit in heart failure, failed to salvage ventricular function in beta(1)AR-mediated cardiomyopathy. PMID: 20443853
  43. beta-Adrenergic receptors transmit sympathetic signals to the ion secretory elements of the colonic epithelial cells stimulating a transient Cl ion secretion and a sustained K ion secretion. PMID: 20413718
  44. This work has provided insight into the intracellular signaling pathways and transcription factors regulating Ncx1 gene expression in a chronically beta-AR-stimulated heart. PMID: 19945464
  45. [beta]1-AR play a significant role in regulating LPS-mediated mortality and inflammation. PMID: 19333138
  46. vasoconstrictor role for vascular alpha1B-adrenoceptors in mouse PMID: 12006674
  47. Pathogenesis of DCM in CREB(A133) mice does not appear to involve abnormal betaAR signaling PMID: 12054854
  48. modulates receptor trafficking and signaling in cardiac myocytes PMID: 12097326
  49. betaAR signaling required for diet-induced thermogenesis and obesity resistance. PMID: 12161655
  50. role in obesity and cold sensitivity and response to fasting PMID: 12387862

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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