Recombinant Mouse Beclin-1 (BECN1) Protein (His-SUMO)

Name: Recombinant Mouse Beclin-1 (BECN1) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-03932P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Mouse Beclin-1 (BECN1) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	O88597
Target Symbol	BECN1
Synonyms	Becn1; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein) [Cleaved into: Beclin-1-C 35 kDa; Beclin-1-C 37 kDa]
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	MEGSKASSSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEEANSGEEPFIETRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQRELKELALEEERLIQELEDVEKNRKVVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQVRYAQIQLDKLKKTNVFNATFHIWHSGQFGTINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLTDKSKELPLYCSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGKIEDTGGSGGSYSIKTQFNSEEQWTKALKFMLTNLKWGLAWVSSQFYNK
Expression Range	1-448aa
Protein Length	Full Length
Mol. Weight	67.6kDa
Research Area	Epigenetics And Nuclear Signaling
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Plays a central role in autophagy. Acts as core subunit of different PI3K complex forms that mediate formation of phosphatidylinositol 3-phosphate and are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis including endosome formation in neuronal cells. May play a role in antiviral host defense.; Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.
Subcellular Location	Cytoplasm. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein. Endosome. Cytoplasmic vesicle, autophagosome.; [Beclin-1-C 35 kDa]: Mitochondrion. Nucleus. Cytoplasm.; [Beclin-1-C 37 kDa]: Mitochondrion.
Protein Families	Beclin family
Database References	KEGG: mmu:56208 STRING: 10090.ENSMUSP00000119369 UniGene: PMID: 29849149 HER2 interacts with Beclin 1 in breast cancer cells and inhibits autophagy. Mice with increased basal autophagy due to a genetically engineered mutation in Becn1 are protected from human HER2-driven mammary tumorigenesis, and HER2 fails to inhibit autophagy in primary cells derived from these mice. PMID: 29610308 Catalase ameliorates diabetes-induced cardiomyopathy through reduced RelA-mediated transcription of BECN1. PMID: 28643395 TRIM50, via the RING domain, ubiquitinates Beclin 1 in a K63-dependent manner enhancing its binding with ULK1 and autophagy activity. PMID: 29604308 findings suggested E. faecalis LTA may increased macrophages autophagy via inhibiting PI3K/Akt/mTOR pathway and this process was Beclin1 dependent. PMID: 29551680 The results suggest that SPK2 interacts with Bcl2 via its BH3 domain, thereby dissociating it from Beclin-1 and activating autophagy while protecting neurons against ischemic injury. PMID: 28682313 alpha-asarone abrogated 7beta-hydroxycholesterol-triggered eIF2alpha-CHOP activation, and enhanced macrophage beclin1 dependent autophagy. PMID: 28088783 These results suggest that the phosphorylated CAV1 functions to activate autophagy through binding to the BECN1/VPS34 complex under oxidative stress and to protect against ischemic damage. PMID: 28542134 Intracerebral hemorrhage contributes to microglia autophagic activation through BECN1 (and ATG5) pathway. PMID: 26732594 These data demonstrate the essential role of Beclin-1 in the formation of autophagosomes and lysosome biogenesis and underline that deletion of this key system is deleterious for cell viability. PMID: 26456737 AMP-activated protein kinase (AMPK) regulates autophagy by phosphorylating BECN1 at Thr388 PMID: 27304906 BECN1/Beclin 1 binding to viral phosphoprotein (P) induced an incomplete autophagy via activating the pathways CASP2-AMPK-AKT-MTOR and CASP2-AMPK-MAPK by decreasing CASP2. PMID: 28129024 proteostasis regulator cysteamine, which rescues the function of the most common F508del-CFTR mutant and hence reduces lung inflammation in CF patients, can also repair the defects of CF macrophages, thus restoring both bacterial internalization and clearance through a process that involves upregulation of the pro-autophagic protein Beclin 1 and re-establishment of the autophagic pathway. PMID: 28079883 Genetically disrupting BECN1-BCL2 binding hyperactivates autophagy in vivo, which sequestrates amyloid oligomers and prevents Alzheimer's disease progression. PMID: 28806762 Data show that 1-month of oral treatment with beta-asarone reduces AChE, Abeta42, APP and Beclin-1 levels and alleviates some behavioral impairments by inhibiting the autophagy via regulating the PI3K/Akt/mTOR pathway in APP/PS1 transgenic mice. The results further support the exploration of beta-asarone as a possible disease-modifying agent for the treatment of Alzheimer's disease. PMID: 27737765 identify FAK as a novel negative regulator of Beclin1-mediated autophagy and indicate that this pathway can facilitate the promotion of compensatory hypertrophic growth PMID: 27994061 The results not only demonstrate that BECN1 as a PI3K-III component is essential for cytokinesis, but also suggest that BECN1 is not associated with autophagy pathway in mouse oocytes. PMID: 27149384 this study shows that knockdown of beclin1 enhances macrophage-associated phagocytosis and intracellular killing of Pseudomonas aeruginosa PMID: 27295610 provide the novel evidence that GADD45A inhibits autophagy via impairing the BECN1-PIK3C3 complex formation PMID: 26636486 in-vitro genetic depletion of beclin-1 in high glucose treated adult rat cardiomyocytes markedly inhibited the level of autophagy and subsequent apoptotic cell death. PMID: 26386349 suggest that neuronal death induced by decreased beclin 1 levels may also be due to impaired trophic factor signaling PMID: 26692002 Beclin 1 is required for neuron viability and regulates endosome pathways via the UVRAG-VPS34 complex. PMID: 25275521 a mechanism of inverse coregulation between BECN1 and MCL1 significantly contributes to their opposing roles in tumorigenesis PMID: 25837021 FKBP5 associates with BECN1, changes its phosphorylation and protein levels and enhances markers of autophagy and autophagic flux PMID: 25714272 identifies a novel negative regulator of autophagy and suggests that RNF216 may be a target for treatment of inflammatory diseases PMID: 25484083 Inverse co-regulation of Beclin 1 and Mcl-1 represents a mechanism of functional counteraction in cancer. PMID: 25472497 Beclin 1-mediated autophagy alleviates cerebral ischemic injury following down-regulation of miRNA-30a. PMID: 24771295 CFTR and BECN1 function can be restored with cysteamine and EGCG in a model of the F508del-CFTR mutation PMID: 25350163 a neuroprotective role for ARRB1, in the context of cerebral ischemia, centered on the regulation of BECN1-dependent autophagosome formation PMID: 24988431 Data show that beclin1 (Becn1) provides essential functions in the ovary that are essential for mammalian reproduction. PMID: 25246579 ALDH2 deficiency exacerbates the pressure overload induced cardiac dysfunction partly by inhibiting Beclin-1 dependent autophagy pathway. PMID: 25086229 These data support a dual role of BECN1 in amyotrophic lateral sclerosis. PMID: 24905722 Becn1 heterozygous knockout mice displayed a prolonged bleeding time and reduced platelet aggregation PMID: 24458007 Our findings demonstrate a novel protective effect of CO in sepsis, dependent on autophagy protein Beclin 1, in a murine model of CLP-induced polymicrobial sepsis PMID: 23971531 Beclin1 is involved, and might be responsible for the crosstalk between autophagy and apoptosis in mouse odontogenesis. PMID: 23793984 Suppression of the Beclin-1-dependent pathway reduced autophagy in pressure-overload heart failure. PMID: 24550138 Rhes robustly binds the autophagy regulator Beclin-1, decreasing its inhibitory interaction with Bcl-2 independent of JNK-1 signaling. PMID: 24324270 Mst1 promoted cardiac dysfunction in mice subjected to myocardial infarction by inhibiting autophagy, associated with increased levels of Thr108-phosphorylated Beclin1. PMID: 24141421 beclin 1 is required for efficient phagocytosis in a microglial cell line and in mouse brains. PMID: 24012002 ATG (BECN1 or ATG7) knockdown compromised mammary epithelial cell-mediated apoptotic body clearance in association with decreased RAC1 activation. PMID: 23380905 Lack of the beclin-binding domain increases autophagy response and activation of NLRP3 inflammasome, which in turn induces a more rapid innate immune response in HSV-1 infection. PMID: 23611643 Caspase 1 activation is protective against hepatocyte cell death by up-regulating beclin 1 protein and mitochondrial autophagy in the setting of redox stress. PMID: 23589298 Attenuation of autophagy by a knockdown of beclin-1 enhanced neurons susceptibility to proapoptotic signals induced by amino acid starvation. PMID: 23011054 IL-2 induced HMGB1 cytoplasmic translocation in MEFs and promoted interaction between HMGB1 and beclin1, which is required for autophagy induction. PMID: 23195496 Aerosol delivery of beclin1 enhanced the anti-tumor effect of radiation in the lungs of K-rasLA1 mice PMID: 22843615 Bim inhibits autophagy by interacting with Beclin 1, an autophagy regulator, an interaction facilitated by LC8. PMID: 22742832 Ischemia/reperfusion injury impairs autophagosome clearance mediated in part by reactive oxygen species-induced decline in lysosome-associated membrane protein-2 and upregulation of BECLIN-1, contributing to increased cardiomyocyte death. PMID: 22592897 in Escherichia coli-containing phagosomes of mouse macrophages, Slamf1 interacts with the class III PI3K Vps34 in a complex with Beclin-1 and UVRAG PMID: 22493499 Beclin 1 coordinates actin dynamics and membrane phospholipid synthesis to promote efficient apoptotic cell engulfment with Rac1 PMID: 22393062 Beclin 1 is involved in regulation of apoptosis and autophagy during replication of ectromelia virus in permissive L929 cells. PMID: 21972018

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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