Recombinant Mouse Annexin A1 (ANXA1) Protein (His)

Name: Recombinant Mouse Annexin A1 (ANXA1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-03369P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Mouse Annexin A1 (ANXA1) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P10107
Target Symbol	ANXA1
Synonyms	Anxa1; Anx1; Lpc-1; Lpc1Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	AMVSEFLKQARFLENQEQEYVQAVKSYKGGPGSAVSPYPSFNVSSDVAALHKAIMVKGVDEATIIDILTKRTNAQRQQIKAAYLQENGKPLDEVLRKALTGHLEEVVLAMLKTPAQFDADELRGAMKGLGTDEDTLIEILTTRSNEQIREINRVYREELKRDLAKDITSDTSGDFRKALLALAKGDRCQDLSVNQDLADTDARALYEAGERRKGTDVNVFTTILTSRSFPHLRRVFQNYGKYSQHDMNKALDLELKGDIEKCLTTIVKCATSTPAFFAEKLYEAMKGAGTRHKALIRIMVSRSEIDMNEIKVFYQKKYGISLCQAILDETKGDYEKILVALCGGN
Expression Range	2-346aa
Protein Length	Full Length of Mature Protein
Mol. Weight	42.6kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Promotes resolution of inflammation and wound healing. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Has no effect on unstimulated T-cells. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton. Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions. Displays Ca(2+)-dependent binding to phospholipid membranes. Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton.
Subcellular Location	Nucleus. Cytoplasm. Cell projection, cilium. Basolateral cell membrane. Lateral cell membrane. Cell membrane; Peripheral membrane protein. Apical cell membrane. Membrane; Peripheral membrane protein. Early endosome. Cytoplasmic vesicle membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Secreted. Secreted, extracellular space. Cell membrane; Peripheral membrane protein; Extracellular side. Secreted, extracellular exosome. Cytoplasmic vesicle, secretory vesicle lumen. Cell projection, phagocytic cup.
Protein Families	Annexin family
Database References	KEGG: mmu:16952 STRING: 10090.ENSMUSP00000025561 UniGene: PMID: 29213013 ANXA1 can be phosphorylated by PKC and is subsequently translocated to the nucleus of BV-2 microglial cells after oxygen glucose deprivation/reoxygenation, resulting in the induction of pro-inflammatory cytokines. PMID: 27426034 Our data indicate that hepatic overexpression of ANX1 and ANX2 inhibits APAP-induced expansion of liver injury PMID: 27451051 The present findings shed light on the role of GILZ in the mechanism of induction of Anxa1 by GCs. As Anxa1 is an important protein for the resolution of inflammatory response, GILZ may represent a new pharmacologic target for treatment of inflammatory diseases. PMID: 28373208 these data indicate that AnxA1 is actively expressed during L. braziliensis infection. PMID: 28289158 Data, including data from studies using knockout mice, suggest cAMP-elevating agents increase levels of AnxA1; this is involved in role of cAMP in resolving acute inflammation (here, ability of cAMP-elevating agents to treat LPS-induced neutrophilic pleurisy). These results reinforce hypothesis that AnxA1 acts in multiple cell types (neutrophils, macrophages) and at multiple levels to promote resolution of inflammation. PMID: 28655761 confirmed the ability of Plg/Pla to both promote efferocytosis and override the prosurvival effect of LPS via annexin A1. PMID: 28320709 this study shows that AnxA1 plays a crucial role in the context of acute gouty inflammation by promoting timely resolution of inflammation PMID: 27995621 Suggest protective role for Annexin A1 in arterial neointima formation in atherosclerosis-prone mice. PMID: 28062503 ANXA1 restores Abeta42-induced blood brain barrier disruption through inhibition of RhoA-ROCK signaling pathway PMID: 27633771 It is proposed that the interactions between ANXA1 and GATA-3 may provide clues to understand the immunosuppression and have potential as new therapeutic targets in immunotherapy after sepsis. PMID: 27833268 AnxA1, is related to the efficiency of the infliximab treatment in experimental colitis, constituting a potential biomarker of therapeutic efficacy PMID: 27343762 these data highlight AnxA1 as a novel determinant of neutrophil maturation and the mechanisms behind blood neutrophil homing to BM via the CXCL12/CXCR4 pathway. PMID: 26892496 We conclude that ANX-A1 is an important regulator of mast cell reactivity to compound 48/80 exerting a negative feedback effect through a mechanism that depends at least partly on the FPR receptor PMID: 26803520 muscle-contraction-induced calpain-dependent ANXA1 fragmentation has a wound-healing effect on damaged cells. This suggested that not the intact form but rather fragmented ANXA1 is a contraction-induced myokine PMID: 26458561 Annexin A1 Deficiency does not Affect Myofiber Repair but Delays Regeneration of Injured Muscles PMID: 26667898 Data show that endothelial annexin A1 from apolipoprotein AI knockout mice was decreased in comparison to that from wild type mice. PMID: 27012521 Anxa1 mRNA and protein is highly expressed in mesenchymal stromal cells (MSCs). Anxa1 KO MSCs cannot enhance glucose stimulated islet insulin secretion. PMID: 26470781 pan antagonist of AnxA1 receptors exacerbated the colitis outcome in TNFR1-/- mice, supporting the pivotal role of AnxA1 in the early recovery PMID: 26386311 knock-out mice are more susceptible to Mycobacterium tuberculosis infection PMID: 25533809 critical regulator of Th17 cell driven autoimmune diseases such as uveitis PMID: 25619792 annexin A1- formyl peptide receptor 2 pathway mediated the insulin resistance of skeletal muscle, as well as systemic insulin sensitivity. PMID: 25616869 Functional role of ANXA1 in tumorigenesis was established in invasion assay where knocking down ANXA1 in murine mammary tumor cell line 168FARN showed lower invasive capability. PMID: 25124533 AnxA1 and Fpr2 have a critical role in the manifestation of adrenal insufficiency in this LPS-induced model, through regulation of cholesterol ester storage PMID: 25818588 The annexin A1 fragment Ac2-26 counteracts conformational activation and clustering of integrins on myeloid cells evoked by CCL5, CCL2, and CXCL1 through inhibiting activation of the small GTPase Rap1. PMID: 25520364 these data indicate that annexin1 is essential in immunity to Mtb infection and mediates the power of DC efferocytosis and cross-presentation. PMID: 25562320 Data (including data from knockout mice) suggest that mobilization of neutrophils during stress requires expression of ANXA1 and activation of glucocorticoid receptor in neutrophils. PMID: 25184992 AnxA1 participates in a paradigm in which malignant prostate epithelial cells that are not cancer stem cells are induced to gain cancer stem cell-like properties. PMID: 24464914 These data show a multiantibody composition in lupus nephritis, where IgG2 autoantibodies against alpha-enolase and annexin AI predominate in the glomerulus and can be detected in serum. PMID: 24790181 findings identify ANXA1 as a proinvasive protein in melanoma that holds promise as a potential prognostic marker and therapeutic target PMID: 24997993 Macrophage-derived AnxA1 plays a functional role in modulating hepatic inflammation and fibrogenesis during nonalcoholic steatohepatitis progression. PMID: 24668763 Data (including data from knockout mice) suggest AnxA1 limits inflammatory response in central (cerebral cortex) and peripheral (mesenteric) microvasculature; estrogen acts independent of AnxA1 to protect cerebral but not mesenteric microvasculature. PMID: 22897118 Data show an increase of extracellular Annexin A1 (ANXA1) starting from the initial phases of skeletal muscle cell differentiation. PMID: 23144744 AX1 can exert diverse, fusogen-specific effects on cell-cell fusion, functioning as an extracellular mediator of differentiation-dependent membrane fusion or as an intracellular promoter of postfusion pore expansion and syncytium formation. PMID: 24648446 The present studies tested the hypothesis that neonatal hyperoxic exposure induces deficits in CCSP expression that are associated with persistent alterations in lung SCGB and ANXA1 expression. PMID: 24187664 ANXA1 plays an important role in TLR activation, leading to an augmentation in the type 1 IFN antiviral cytokine response. PMID: 24048896 In mice, annexin 1 prevented the development of inflammatory dendritic cells and suppressed the cellular immune response against the model antigen ovalbumin expressed in apoptotic cells. PMID: 23638088 we suggest that MIF counter-regulates Dexamethasone-induced annexin 1 expression PMID: 23777345 Results indicate that the AnxA1/FPR2 system has an important role in effecting the resolution of cerebral inflammation in sepsis and may provide a novel therapeutic target. PMID: 22964301 T-cell-expressed AnxA1 functions to attenuate T-cell-driven inflammatory responses via T-cell-intrinsic effects on intracellular signaling, proliferation, and Th1/Th17 cytokine release. PMID: 23267026 revealed annexin A1 to be an effective regulator in tumor stroma and suggested a mechanism that annexin A1 affects tumor development and metastasis PMID: 23077482 ANXA1(-/-) mice exhibit significantly increased BBB permeability as a result of disrupted interendothelial cell tight junctions, essentially related to changes in the actin cytoskeleton, which stabilizes tight and adherens junctions. PMID: 23277546 a novel epithelial FPR1/NOX1-dependent redox signaling pathway that promotes mucosal wound repair. PMID: 23241962 data raise the possibility that a reduction in plasma AnxA1 may contribute to the chronic inflammatory phenotype observed in human obesity PMID: 23038751 The data showed that annexin A1 exerts an inhibitory effect on COX-2 expression in the macula densa. ANXA1 may be a novel intrinsic modulator of renal juxtaglomerular regulation by inhibition of PGE2 synthesis. PMID: 22791338 Data suggest that Annexin 1 (ANXA1) plays a protective role in DNA damage and modulates cell adhesion and motility, indicating its potential role in cancer initiation as well as progression in breast carcinoma. PMID: 22511458 AnxA1 is an endogenous determinant for the therapeutic efficacy of Dexamethasone in inflammatory arthritis. PMID: 22562975 AnxA1 mediates natural and glucocorticoid-induced resolution of inflammation with profound effects on neutrophil apoptosis. PMID: 22493082 Cleavage of ANXA1 during mast-cell activation causes proinflammatory reactions by increasing the phosphorylation of cytosolic phospholipase A2 (cPLA2) and production of eicosanoids. PMID: 22539796 ANXA1 is an important regulatory factor in the development of allergic disease and dysregulation of its expression can lead to pathological changes which may affect disease progression. PMID: 22092555

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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