Recombinant Mouse Acyl-Coa Desaturase 1 (SCD1) Protein (His)

Name: Recombinant Mouse Acyl-Coa Desaturase 1 (SCD1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-07929P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Mouse Acyl-Coa Desaturase 1 (SCD1) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P13516
Target Symbol	SCD1
Synonyms	Scd1; Acyl-CoA desaturase 1; Delta(9-desaturase 1; Delta-9 desaturase 1; Fatty acid desaturase 1; Stearoyl-CoA desaturase 1
Species	Mus musculus (Mouse)
Expression System	in vitro E.coli expression system
Tag	N-10His
Target Protein Sequence	MPAHMLQEISSSYTTTTTITAPPSGNEREKVKTVPLHLEEDIRPEMKEDIHDPTYQDEEGPPPKLEYVWRNIILMVLLHLGGLYGIILVPSCKLYTCLFGIFYYMTSALGITAGAHRLWSHRTYKARLPLRIFLIIANTMAFQNDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGGKLDMSDLKAEKLVMFQRRYYKPGLLLMCFILPTLVPWYCWGETFVNSLFVSTFLRYTLVLNATWLVNSAAHLYGYRPYDKNIQSRENILVSLGAVGEGFHNYHHTFPFDYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKATVLARIKRTGDGSHKSS
Expression Range	1-355aa
Protein Length	Full Length
Mol. Weight	43.9 kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Stearoyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Catalyzes the insertion of a cis double bond at the Delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Gives rise to a mixture of 16:1 and 18:1 unsaturated fatty acids. Plays an important role in lipid biosynthesis. Plays an important role in regulating the expression of genes that are involved in lipogenesis and in regulating mitochondrial fatty acid oxidation. Plays an important role in body energy homeostasis. Contributes to the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides. Required for normal development of sebaceous glands. Required for the biosynthesis of normal levels of Delta-9 unsaturated fatty acids and 1-alkyl-2,3-diacylglycerol in the Harderian gland. Required for normal production of meibum, an oily material that prevents drying of the cornea.
Subcellular Location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane.
Protein Families	Fatty acid desaturase type 1 family
Database References	KEGG: mmu:20249 STRING: 10090.ENSMUSP00000036936 UniGene: PMID: 30063922 Experimental models in mice and human epithelial cells suggest that inhibition of stearoyl-coenzyme A desaturase activity leads to airway hyper-responsiveness and reduced antiviral defense. PMID: 28397284 leucine deprivation induces the expression of miR-212-5p in a GCN2/ATF4-dependent manner. miR-212-5p suppresses lipid accumulation in liver by targeting FAS and SCD1 under both normal diet and high-fat diet conditions. PMID: 28667176 The aggravated steatosis in Trif(-/-) mice was due to the increased hepatocyte transcription of the gene encoding stearoyl-coenzyme A (CoA) desaturase 1 (SCD1), the rate-limiting enzyme for lipogenesis. PMID: 28790196 A positive correlation among EGFR activation, SCD1 Y55 phosphorylation and SCD1 protein expression. PMID: 28724430 BTG1 has a role in regulating hepatic lipid metabolism and in preventing ATF4 and SCD1 from inducing liver steatosis PMID: 27188441 SCD1 is a key player in the development of alcoholic fatty liver disease. PMID: 27477676 SCD1 ablation/inhibition decreased cardiac lipid content. PMID: 27751891 Our data provide evidence that SCD1 has a broad impact on WAT lipid handling by altering TAG composition in a depot-specific manner, reducing FA reesterification, and regulating markers of lipolysis and glyceroneogenesis. PMID: 28659287 SCD expressed by HSCs promoted liver tumor development in mice. PMID: 28143772 investigated the effect of genetic ablation of SCD1 in 3T3-L1 adipocytes on membrane microdomain lipid composition at the species-specific level PMID: 27632198 Splenic effector T cells (CD4(+)CD25(-)) from age- and sex-matched wild-type (WT) and Scd1KO mice were isolated by FACS and intraperitoneally administered to Rag1KO mice, which were monitored for the development of colitis. At day 60 postcell transfer, Rag1KO mice that received Scd1KO CD4(+)CD25(-) T cells displayed accelerated and exacerbated colitis than mice receiving WT CD4(+)CD25(-) T cells. PMID: 27609767 Scd1 protects cells against lipotoxicity-mediated apoptosis in proximal tubular cells. PMID: 27834856 Scd1 Is Regulated by Dbc1 and SirT1. PMID: 26074075 Study provides new insights into the critical role of SCD1 as a regulator of adipocyte function and lipid metabolism. PMID: 25959085 The study reports report the 3.25-A crystal structure of human SCD1 in complex with its substrate, stearoyl-coenzyme A, which defines the new SCD1 dimetal catalytic center and reveals the determinants of substrate binding. PMID: 26098317 SCD1 is a novel oncogenic factor specifically required for tumor cell viability in anaplastic thyroid carcinoma. PMID: 25675381 crystal structure of mouse SCD1 bound to stearoyl-CoA at 2.6 A resolution PMID: 26098370 Data indicate that lowering stearoyl-CoA desaturase-1 (SCD-1) activity specifically activates p38 mitogen-activated protein kinase (P38MAPK). PMID: 25678624 Results provide new mechanistic insight into the role of SCD1 as a regulator of fatty acid profiles and Triacylglycerol (TG) synthesis in adipocytes, and reinforce that modulating SCD1 activity may help reduce the risk of obesity-related complications. PMID: 24566853 Data indicate that global DNA methylation level in adipocytes depends on stearoyl-CoA desaturase 1 (SCD1) expression/activity. PMID: 25130896 Results show that SCD1 was expressed selectively in the S3 segment of proximal tubule cells, and SCD1 abundance was increased in uromodulin-associated kidney disease affected kidneys. PMID: 25409434 Data indicate that hepatic stearoyl-CoA desaturase 1 (SCD1) expression was decreased in cytochrome P450 1B1 (CYP1B1)-null mice. PMID: 24684199 Data show that liver X receptor (LXR) agonist TO901317 increases hepatic fatty acid desaturation via induction of stearoyl CoA desaturase-1 (SCD1) expression in an LXRalpha-dependent and sterol regulatory element-binding protein 1c (SREBP1c)-mediated manner. PMID: 23945440 results suggest that SCD1 activity is required for the earliest step of autophagosome formation PMID: 25023287 mRNA and protein levels of stearoyl-coenzyme A desaturase 1 are significantly elevated in the tsc2(-/-) MEFs compared with Tsc2(+/+) mouse embryonic fibroblasts PMID: 24296537 Hepatic expression of Scd1 is differentially affected by carbohydrate- and lipid content of the diet. PMID: 23803969 This minireview summarizes the role of skin SCD1 in regulating skin integrity and whole body energy homeostasis and offers a discussion of potential pathways that may connect these seemingly disparate phenotypes. PMID: 24356954 SCD1 overexpression is associated with increased muscle triglyceride content. PMID: 23918045 Increased levels of SCD1 enhance osteogenesis in bone marrow-mesenchymal stem cells. PMID: 24088802 The protective effect of TO901317 was lost after gene silencing of Scd-1/-2, thereby confirming that the protective effect of TO901317 is mediated by Scd-1/-2. PMID: 23867797 Gamma-tocotrienol showed attenuation of triglyceride through effect on fatty acid synthase, sterol regulatory element-binding transcription factor 1, stearoyl CoA desaturase 1, and carnitine palmitoyl transferase 1A gene expression in Hepa 1-6 cells. PMID: 23727646 Monounsaturated fatty acids generated via stearoyl CoA desaturase-1 are endogenous inhibitors of fatty acid amide hydrolase. PMID: 24191036 Systemic down-regulation of delta-9 desaturase promotes muscle oxidative metabolism and accelerates muscle function recovery following nerve injury. PMID: 23785402 In vivo studies using germline (Creb1(-/-) ) and lung epithelial-specific (Creb1(EpiDelta/Delta) ) Creb1 knockout mice showed strongly reduced Scd1, but not Fasn gene expression and protein levels in lung epithelial cells. PMID: 23637738 macrophage-specific SCD1 overexpression promotes overall RCT through increased cholesterol efflux to HDL, suggesting that macrophage SCD1 achieves an anti-atherogenic effect by enhancing RCT. PMID: 23747827 a critical role for Scd1 in functional regulation of leukemia stem cells PMID: 22431519 Thyroid hormone negatively regulates human SCD-1 gene expression in without direct binding of the TH receptor to the SCD-1 gene promoter. PMID: 23221600 SCD1 deletion from adipose and/or liver is insufficient to elicit protection from obesity PMID: 22669918 analysis of potent and liver-selective stearoyl-CoA desaturase (SCD) inhibitors in an acyclic linker series PMID: 22101133 Repression of SCD1 by alpha-linolenic acid favorably increased cholesterol efflux and decreased cholesterol accumulation in foam cells. PMID: 21658928 aryl hydrocarbon receptor regulation of Scd1 not only alters lipid composition but also contributes to the hepatotoxicity of 2,3,7,8-tetrachlorodibenzo-rho-dioxin. PMID: 21890736 Overexpression of KLF15 transcription factor in adipocytes of mice results in down-regulation of SCD1 protein expression in adipocytes and consequent enhancement of glucose-induced insulin secretion. PMID: 21862590 Skin-specific Scd1 knockout (SKO) mice still remain resistant to obesity. PMID: 21573029 TR4 transactivation is inhibited via phosphorylation by metformin-induced AMP-activated protein kinase (AMPK) at the amino acid serine 351, which results in the suppression of SCD1 gene expression. PMID: 21478464 SCD-1 activity is required for efficient cholesterol esterification to monounsaturated fatty acids and that loss of its activity increases Xbp-1s-mediated free cholesterol synthesis. PMID: 20923962 Data from gene knockdown experiments suggest Scd1 is important in the beta-cell response to physiological stress. PMID: 20920255 adipose-specific deletion of SCD1 induces GLUT1 up-regulation in adipose tissue, suggesting that GLUT1 may play a critical role in controlling glucose homeostasis of adipose tissue in adipose-specific SCD1-deficient conditions. PMID: 20655875 Data suggest that the increased food intake and increased energy expenditure of Scd1-/- mice are independent of the neuropeptide melanin-concentrating hormone. PMID: 19883709 findings show show that stearoyl-CoA desaturase-SCD is involved in beta cell cytoprotective mechanism to fatty acid toxicity through its ability to transfer saturated FA into monounsaturated FA that are incorporated in lipids PMID: 19787047

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.