Recombinant Mouse 25-Hydroxyvitamin D-1 Alpha Hydroxylase, Mitochondrial (CYP27B1) Protein (His)

Name: Recombinant Mouse 25-Hydroxyvitamin D-1 Alpha Hydroxylase, Mitochondrial (CYP27B1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-09161P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Mouse 25-Hydroxyvitamin D-1 Alpha Hydroxylase, Mitochondrial (CYP27B1) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	O35084
Target Symbol	CYP27B1
Synonyms	Cyp27b1; Cyp27b; Cyp4025-hydroxyvitamin D-1 alpha hydroxylase; mitochondrial; EC 1.14.15.18; 25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D(3) 1-alpha-hydroxylase; VD3 1A hydroxylase; Calcidiol 1-monooxygenase; Cytochrome P450 subfamily XXVIIB polypeptide 1; Cytochrome P450C1 alpha; Cytochrome P450VD1-alpha; Cytochrome p450 27B1
Species	Mus musculus (Mouse)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	MTQAVKLASRVFHRIHLPLQLDASLGSRGSESVLRSLSDIPGPSTLSFLAELFCKGGLSRLHELQVHGAARYGPIWSGSFGTLRTVYVADPTLVEQLLRQESHCPERCSFSSWAEHRRRHQRACGLLTADGEEWQRLRSLLAPLLLRPQAAAGYAGTLDNVVRDLVRRLRRQRGRGSGLPGLVLDVAGEFYKFGLESIGAVLLGSRLGCLEAEVPPDTETFIHAVGSVFVSTLLTMAMPNWLHHLIPGPWARLCRDWDQMFAFAQRHVELREGEAAMRNQGKPEEDMPSGHHLTHFLFREKVSVQSIVGNVTELLLAGVDTVSNTLSWTLYELSRHPDVQTALHSEITAGTRGSCAHPHGTALSQLPLLKAVIKEVLRLYPVVPGNSRVPDRDIRVGNYVIPQDTLVSLCHYATSRDPTQFPDPNSFNPARWLGEGPTPHPFASLPFGFGKRSCIGRRLAELELQMALSQILTHFEVLPEPGALPIKPMTRTVLVPERSINLQFVDR
Expression Range	1-507aa
Protein Length	Full Length
Mol. Weight	60.2kDa
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	A cytochrome P450 monooxygenase involved in vitamin D metabolism and in calcium and phosphorus homeostasis. Catalyzes the rate-limiting step in the activation of vitamin D in the kidney, namely the hydroxylation of 25-hydroxyvitamin D3/calcidiol at the C1-alpha position to form the hormonally active form of vitamin D3, 1alpha,25-dihydroxyvitamin D3/calcitriol that acts via the vitamin D receptor (VDR). Has 1-alpha-hydroxylase activity on vitamin D intermediates of the CYP24A1-mediated inactivation pathway. Converts 24R,25-dihydroxyvitamin D3/secalciferol to 1-alpha,24,25-trihydroxyvitamin D3, an active ligand of VDR. Also active on 25-hydroxyvitamin D2. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin.
Subcellular Location	Mitochondrion membrane.
Protein Families	Cytochrome P450 family
Database References	KEGG: mmu:13115 STRING: 10090.ENSMUSP00000130005 UniGene: Mm.6216
Tissue Specificity	Kidney.

Gene Functions References

Vascular Calcification Induced by Chronic Kidney Disease Is Mediated by an Increase of 1alpha-Hydroxylase Expression in Vascular Smooth Muscle Cells PMID: 27074284
These results reveal that differential regulation of Cyp27b1 expression represents a mechanism whereby 1,25(OH)2D3 can fulfill separate functional roles, first in the kidney to control mineral homeostasis and second in extra-renal cells to regulate target genes linked to specific biological responses. PMID: 28808057
The data indicate that abnormal osteoclastogenesis due to the absence of CYP27B1 expression, consistent with the notion that endogenous metabolism of 25-hydroxyvitamin D optimizes osteoclastogenesis and ameliorates the resulting activity of mature osteoclasts. PMID: 26639637
The absence of 25-hydroxyvitamin D3-1alpha-hydroxylase potentiates the suppression of EAE in mice by ultraviolet light. PMID: 27108944
Cyp27b1(-/-) mice exhibited hypocalcemia, growth defects, and skeletogenesis dysfunction, similar to Vdr(-/-) mice, but do not display alopecia PMID: 28025137
Findings demonstrate that in-tumor CYP27B1 1-alpha-hydroxylase activity plays a crucial role in controlling early oncogene-mediated mammary carcinogenesis events, at least in part by modulating tumoral cell NF-kappaB p65 nuclear translocation. PMID: 27119753
the effect of 25-hydroxyvitamin D-1-alpha-hydroxylase on the atherosclerosis disease both in apolipoprotein (apo) E-/- mice and wild-type mice, was investigated. PMID: 28178628
P450C1 alpha deficiency results in abnormal calcium handling and cardiac dysfunction in mice with defective vitamin D signaling. PMID: 25268137
Throughout the male reproductive tract specific bands of CYP27B1 are determined. PMID: 23188491
observed a 3- to 10-fold increase in CYP27B1 mRNA abundance in the lung, spleen, aorta and testis of FGF-23 null/1alpha-Luc mice PMID: 24019880
Data show that the absence of either of the two key hydroxylases, vitamin D 25-hydroxylase (CYP2R1) or vitamin D 25-hydroxyvitamin D-1alpha-hydroxylase (CYP27B1)neither inhibits nor enhances the development of experimental autoimmune encephalomyelitis (EAE). PMID: 22592802
These results confirm the expression of vitamin D receptor and Cyp27b1 in vivo and suggest a potential role for vitamin D(3) in skeletal muscle regeneration following injury. PMID: 22648952
Bone formation parameters were increased significantly in all pups fed by dams on the rescue diet but were still lower in 1alpha(OH)ase(-/-) pups than in 1alpha(OH)ase(+/-) pups. PMID: 21791625
1alpha-hydroxylase may have a role in enhanced radiosensitivity PMID: 21343672
regulation of bone CYP27B1 is unique from that in the kidney, and may play an important role in bone formation PMID: 20236619
study shows CYP27B1 can hydroxylate 25-hydroxyvitamin D2 & 25-hydroxyvitamin D3 associated with phospholipid membranes; low activity found at higher membrane levels of 25-hydroxyvitamin D; substrate inhibition may contribute to enzyme activity regulation PMID: 20193763
both 1alphaOHase gene ablation and Pi supplementation inhibit renal calcification in Npt2-/- mice and that 1,25(OH)2D is essential for the development of hypercalciuria and nephrocalcinosis in the mutant strain. PMID: 14656762
leptin suppresses renal gene overexpression for 1 alpha-hydroxylase and 24-hydroxylase and corrects increased serum concentrations of calcium and phosphate in ob/ob mice. PMID: 14657008
1 alpha-OHase knockout mice fed a normal Ca2+ diet developed severe hypocalcemia, rickets and died with an average life span of 12 +/- 2 weeks PMID: 14717923
25OHD 1OHase essential for normal epidermal differentiation, most likely by producing vitamin D metabolite, 1,25(OH)(2)D, responsible for inducing proteins regulating calcium levels in epidermis critical for generation and maintenance of barrier. PMID: 15102089
5' flanking region of the gene directs expression to the proximal convoluted tubules of the kidney PMID: 15691891
study of substrate recognition of CYP27B1 PMID: 15972816
CYP27B1 null mice with LacZreporter gene display no 25-hydroxyvitamin D3-1alpha-hydroxylase promoter activity in the skin. PMID: 16371465
These results suggest that adrenodoxin functions as an effector for the oxygen transfer reaction in addition to being an electron donor for CYP27B1. PMID: 16584176
confirm a crucial role for STAT1alpha as well as for C/EBPbeta in the regulation of 1alpha-hydroxylase in monocytes PMID: 17267208
leptin attenuates renal 1alpha-hydroxylase gene expression through ObRb. Furthermore, leptin appears to act indirectly on renal proximal tubules to regulate 1alpha-hydroxylase gene expression. PMID: 17400175
FGF-23 directly regulates renal 1alpha-hydroxylase gene expression via activation of the ERK1/2 signaling pathway. PMID: 17699549
Neither high dietary Ca nor high dietary vitamin D3 is sufficient to fully recover the phenotype of CYP27B1 KO mice. PMID: 18029472
Results demonstrate that the proximal 1.5kb 5' flanking region of the CYP27B1 gene directs the expression of CYP27B1 in a number of known and novel tissues in a specific manner. PMID: 18313834
Lack of Cyp27b1 exacerbates disease in mouse model, suggesting that similar effects may occur with vitamin D deficiency PMID: 18535110
1alpha-hydroxylase is expressed in adipose tissue and is functional in cultured adipocytes. PMID: 18840526
Results from genetic manipulation of expression of Cyp27b1 in chondrocytes support direct role for locally synthesized 1,25(OH)2D3, acting through the vitamin D receptor, in vascular invasion & osteoclastogenesis during endochondral bone development. PMID: 19477943

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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