Recombinant Mouse 14-3-3 zeta Protein

Name: Recombinant Mouse 14-3-3 zeta Protein
Brand: Beta LifeScience
SKU: BL-1532SG
Availability: InStock

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Tag	His
Host Species	Mouse
Accession	NM_011740
Synonym	Ywhaz; 1110013I11Rik; 14-3-3zeta; AI596267
Background	14-3-3 zeta (also known as tyrosine 3-monooxygenase /tryptophan 5-monooxygenase activation protein, zeta polypeptide) is a member of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 zeta protein plays a key role in cancer biology by being an important regulator of major cellular processes such as proliferation, differentiation, senescence and apoptosis (1). 14-3-3 zeta protein has been shown to interact with the IRS1 protein, suggesting a role for this protein in regulating insulin sensitivity by interrupting the association between the insulin receptor and IRS1 (2).
Description	Recombinant full-length mouse 14-3-3 zeta was ex-pressed by baculovirus in Sf9 insect cells, fused with a His tag at N-terminus.
Source	Sf9 insect cells
AA Sequence	Full Length
Molecular Weight	31 kDa
Purity	For specific purity information on a given lot, see related COA.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability	The recombinant protein is stable for up to 12 months at -70°C
Usage	For Research Use Only
Storage	Recombinant Mouse 14-3-3 zeta Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation. In neurons, regulates spine maturation through the modulation of ARHGEF7 activity.
Subcellular Location	Cytoplasm. Melanosome.
Protein Families	14-3-3 family
Database References	KEGG: mmu:22631 STRING: 10090.ENSMUSP00000022894 UniGene: PMID: 28494163 These results demonstrate that miR-146a enhances class switch and secretion of IgE in B cells by upregulating 14-3-3sigma expression, and suggest that miR-146a may be a potential target for asthma therapy. PMID: 29101850 When taken together these findings demonstrate novel roles of 14-3-3zeta in the regulation of glucose homeostasis and suggest that modulating 14-3-3zeta levels in intestinal L cells may have beneficial metabolic effects through GLP-1-dependent mechanisms. PMID: 27167773 Results indicate that HuR induces 14-3-3zeta translation via interaction with its 3' UTR and that 14-3-3zeta is necessary for stimulation of intestinal epithelial cell migration after wounding. PMID: 27401462 PKCdelta-mediated phosphorylation of 14-3-3zeta contributes to the nuclear retention of FOXO1, even when FOXO1 is phosphorylated as under non-stress conditions PMID: 26363783 14-3-3zeta deficient mice in the BALB/c background display behavioral and anatomical defects associated with Schizophrenia-like disorder. PMID: 26207352 Ywhaz coordinates adipogenesis in visceral fat. PMID: 26220403 An essential role for 14-3-3 zeta in the regulation of macropinocytosis in macrophages upon cytokine stimulation through modulation of the localization of coronin 1. PMID: 25645340 In the majority of NAFLD treatment groups and time points the most stable gene was YWHAZ. PMID: 22583519 By preventing the inactivation of cofilin, metabolic stress-induced degradation of 14-3-3zeta promotes the conversion of blood monocytes into a hypermigratory, proatherogenic phenotype. PMID: 24812321 14-3-3zeta KO mice displayed enhanced locomotor hyperactivity induced by the dopamine (DA) releaser amphetamine. Consistent with 14-3-3zeta having a role in DA signalling, we found increased levels of DA in the striatum of 14-3-3zeta KO mice. PMID: 24301645 The activated serine/threonine kinase Akt/protein kinase B phosphorylated BTK on two sites prior to 14-3-3 zeta binding. PMID: 23754751 studies demonstrate that increased 14-3-3zeta levels protect against ER stress and seizure-damage despite down-regulation of the unfolded protein response PMID: 23359526 Multiple tumor-associated microRNAs modulate the survival and longevity of dendritic cells by targeting YWHAZ and Bcl2 signaling pathways. PMID: 23355742 14-3-3 zeta as the only prognosticator of local recurrence-free survival (LRFS) and also an independently predicted factor of disease-specific survival (DSS). PMID: 21322651 results suggest that 14-3-3zeta, a novel direct binding partner of the L1ICD, promotes L1 phosphorylation by CKII in the central nervous system, and regulates neurite outgrowth, an important biological process triggered by L1 PMID: 20976158 Mutation of the 14-3-3 zeta phosphorylation sites in Smad3 markedly reduced the 14-3-3-zeta-mediated inhibition of TGF-beta1-induced p15 promoter-reporter activity & cell cycle arrest in mammary epithelial cells. PMID: 20082218 an essential role of 14-3-3zeta as a mediator of the proerythroid differentiation actions of miR-451 PMID: 20679397 in miR-144/451(-/-) erythroblasts, 14-3-3zeta accumulates, causing partial relocalization of FoxO3 from nucleus to cytoplasm with dampening of its transcriptional prog PMID: 20679398 14-3-3zeta controls CCTalpha nuclear import in response to calcium signals, thereby regulating mammalian phospholipid synthesis. PMID: 20007511 14-3-3 binds to phosphorylated Raf-1, blocking Raf-1 recruitment to the plasma membrane and preventing its activation PMID: 12801936 14-3-3zeta has a role in MAPKAPK2-mediated phosphorylation, which may represent a novel pathway mediating p38 MAPK-dependent inflammation PMID: 12861023 dimeric versus monomeric status of 14-3-3zeta is controlled by phosphorylation of Ser58 at the dimer interface PMID: 12865427 14-3-3 was identified as a binding partner of MITF in osteoclast precursors, and overexpression of 14-3-3 in a transgenic model resulted in increased cytosolic localization of MITF and decreased expression of MITF target genes [14-3-3 zeta] PMID: 16822840 14-3-3:Shc scaffolds can act as multivalent signaling nodes for the integration of both phosphoserine/threonine and phosphotyrosine pathways to regulate specific cellular responses. PMID: 19218246 Caspase-2 phosphorylated at S135 binds 14-3-3zeta, thus preventing C2 dephosphorylation. PMID: 19531356

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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Recombinant Mouse 14-3-3 zeta Protein

Recombinant Mouse 14-3-3 zeta Protein

Product Overview

Target Details

FAQs