Recombinant Human Zona Pellucida Sperm-Binding Protein 3 (ZP3) Protein (GST)

Name: Recombinant Human Zona Pellucida Sperm-Binding Protein 3 (ZP3) Protein (GST)
Brand: Beta LifeScience
SKU: BLC-08280P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Zona Pellucida Sperm-Binding Protein 3 (ZP3) Protein (GST) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P21754
Target Symbol	ZP3
Synonyms	HUMZP3; Processed zona pellucida sperm-binding protein 3; Sperm receptor; zona pellucida glycoprotein 3 (sperm receptor); Zona pellucida glycoprotein 3; zona pellucida glycoprotein 3A (sperm receptor); Zona pellucida glycoprotein ZP3; Zona pellucida protein C; Zona pellucida sperm binding protein 3 precursor; Zp-3; ZP3 372; ZP3 424; ZP3 474; ZP3; ZP3_HUMAN; ZP3A; ZP3A/ZP3B; ZP3B; ZPC
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-GST
Target Protein Sequence	QPLWLLQGGASHPETSVQPVLVECQEATLMVMVSKDLFGTGKLIRAADLTLGPEACEPLVSMDTEDVVRFEVGLHECGNSMQVTDDALVYSTFLLHDPRPVGNLSIVRTNRAEIPIECRYPRQGNVSSQAILPTWLPFRTTVFSEEKLTFSLRLMEENWNAEKRSPTFHLGDAAHLQAEIHTGSHVPLRLFVDHCVATPTPDQNASPYHTIVDFHGCLVDGLTDASSAFKVPRPGPDTLQFTVDVFHFANDSRNMIYITCHLKVTLAEQDPDELNKACSFSKPSNSWFPVEGSADICQCCNKGDCGTPSHSRRQPHVMSQWSRS
Expression Range	23-346aa
Protein Length	Partial
Mol. Weight	63.0kDa
Research Area	Developmental Biology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.
Subcellular Location	[Processed zona pellucida sperm-binding protein 3]: Zona pellucida.; Cell membrane; Single-pass type I membrane protein.
Protein Families	ZP domain family, ZPC subfamily
Database References	HGNC: 13189 OMIM: 182889 KEGG: hsa:7784 STRING: 9606.ENSP00000378326 UniGene: PMID: 30341457 The oocyte ZP3 expression was the main predictor of the fertilization capacity. PMID: 28204536 Missense Mutation in ZP3 gene is associated with Empty Follicle Syndrome and Female Infertility. PMID: 28886344 Epididymal CRISP1 mediates sperm-zona pellucida binding through its interaction with ZP3. PMID: 24334245 observed sequence variations in exons of ZP3 gene in women with infertility of unknown origin who exhibit abnormal zona pellucida; sperm-ovum interactions appear relatively normal in these patients [CASE REPORTS] PMID: 22889493 Eggs expressing glycoprotein huZP3, derived from transgenic mice, bind murine but not human sperm, implying that huZP3 acquires the same O-glycans as native mZP3. PMID: 14673092 human and mouse ZP3 proteins are quite similar, and alternative explanations of taxon-specific sperm binding warrant exploration PMID: 15379548 Binding sites for recombinant zona pellucida C (ZPC) glycoprotein are located both at the N- and C-terminus of proacrosin PMID: 15950651 Peptides rhuZP3a22 approximately 176 and rhuZP3b177 approximately 348 have a role similar to human ZP3. The mechanism of the response to the peptides involves influx of calcium, the G protein pathway, and a T-type calcium channel. PMID: 17192598 may have a role in the development of primordial follicle before zona pellucida formation PMID: 18502569 induces acrosome reactions which are protein kinase-C, protein tyrosine kinase, T-type Ca2+ channels, and extracellular Ca2+ dependent PMID: 18667750 a significant decrease in acrosomal exocytosis mediated by both recombinant human ZP3 (p<0.005) and ZP4 (p<0.005) was observed in presence of the immune sera PMID: 19004505 The functional activity of human ZP3 resides in its C-terminal domain. PMID: 19246320

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.