Recombinant Human WHSC1L1 (NSD3) Protein
Beta LifeScience
SKU/CAT #: BL-1026SG
Recombinant Human WHSC1L1 (NSD3) Protein
Beta LifeScience
SKU/CAT #: BL-1026SG
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Product Overview
Tag | GST |
Host Species | Human |
Accession | BC113469 |
Synonym | NSD3, DC28, FLJ20353, pp14328 |
Background | Histone-lysine N-methyltransferase NSD3 or Wolf-Hirschhorn syndrome candidate 1-like protein 1 (WHSC1L1) is a nuclear receptor binding SET domain (NSD) protein that belongs to a family of three histone methyltransferases including NSD1, NSD2/MMSET/WHSC1, and NSD3/WHSC1L1. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression. |
Description | Recombinant human WHSC1L1 (NSD3) (1038-end) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods. |
Source | Sf9 insect cells |
AA Sequence | 1038a.a.-end |
Molecular Weight | ~72 kDa |
Purity | For specific purity information on a given lot, see related COA. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Formulation | Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol. |
Stability | The recombinant protein is stable for up to 12 months at -70°C |
Usage | For Research Use Only |
Storage | Recombinant Human WHSC1L1 (NSD3) Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | Histone methyltransferase. Preferentially dimethylates 'Lys-4' and 'Lys-27' of histone H3 forming H3K2me2 and H3K27me2. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression. |
Subcellular Location | Nucleus. Chromosome. |
Protein Families | Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, SET2 subfamily |
Database References | |
Associated Diseases | Defects in NSD3 may be involved in non small cell lung carcinomas (NSCLC). Amplified or overexpressed in NSCLC. |
Tissue Specificity | Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung. |
Gene Functions References
- Studies showed that depletion of NSD3 in osteosarcoma cell lines inhibited cell proliferation and survival, and induced cell apoptosis. RNA sequence analysis of tumor cell lines with NSD3 deletion revealed that NSD3 functions as either a transcriptional activator or a repressor. These results suggest that NSD3 may function as an oncogenic driver in osteosarcoma. PMID: 28901481
- Results extend the in vitro results and show that targeted expression of NSD3 to the mammary gland of FVB mice is oncogenic, consistent with the hypothesis that NSD3 is an important driver oncogene in human breast cancer. PMID: 28484924
- WHSC1L1 and H3K36me2 are enriched in the gene bodies of the cell cycle-related genes CDC6 and CDK2, implying that WHSC1L1 directly regulates the transcription of these gene PMID: 27285764
- This study demonstrates that over-expression of WHSC1L1 is linked to over-expression of ER-alpha in SUM-44 breast cancer cells and in primary human breast cancers. PMID: 27005559
- Studies indicate that the NSD methyltransferases NSD1, NSD2/WHSC1/MMSET and NSD3/WHSC1L1 were overexpressed, amplified or somatically mutated in multiple types of cancer, suggesting their critical role in cancer. PMID: 25942451
- Results demonstrate that the AML maintenance function of BRD4 requires its interaction with the short isoform of NSD3 lacking the methyltransferase domain. This protein is an adaptor that sustains leukemia by linking BRD4 to the CHD8 chromatin remodeler. PMID: 26626481
- The results describe the binding of NSD1, 2 and 3 catalytic domains (CD) on histone tails through recognition of histone-lysine and methylation properties. PMID: 25494638
- The involvement of the NSD3 methyltransferase as a component of the NUT fusion protein oncogenic complex identifies a new potential therapeutic target. PMID: 24875858
- PPAPDC1B and WHSC1L1 played a major role in regulating the survival of breast cancer, pancreatic adenocarcinoma and small-cell lung cancer-derived cell lines. PMID: 24051013
- Data indicate that siRNA attenuated the expression levels of CCNG1 and NEK7, implying that WHSC1L1 appears to activate the expression of CCNG1 and NEK7 in cancer cells. PMID: 23011637
- methyltransferase NSD3 has chromatin-binding motifs, PHD5-C5HCH, that are distinct from other NSD (nuclear receptor SET domain) family members in their histone H3 recognition PMID: 23269674
- Functional studies with Brd4 indicate that the ET domain mediates pTEFb-independent transcriptional activation through a subset of these associated factors, including NSD3. PMID: 21555454
- Overexpression of WHSC1L1 gene is associated with breast cancer. PMID: 20940404
- NSD3L depletion increased the invasiveness of MDA-MB-231 breast cancer cells indicating that NSD3L normally restrain cellular metastatic potential. Together the presented data indicates that NSD3L is a candidate tumor suppressor. PMID: 20599755