Recombinant Human Vitamin K-Dependent Protein S (PROS1) Protein (His-SUMO)

Name: Recombinant Human Vitamin K-Dependent Protein S (PROS1) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-03093P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Description	Recombinant Human Vitamin K-Dependent Protein S (PROS1) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P07225
Target Symbol	PROS1
Synonyms	Preproprotein S; Propiece of latent protein S; PROS 1; PROS; PROS_HUMAN; proS1; Protein S alpha; Protein Sa; PS 21; PS 22; PS 23; PS 24; PS 25; PS 26; PS21; PS22; PS23; PS24; PS25; PS26; PSA; THPH5; THPH6; Vitamin K dependent protein S; Vitamin K-dependent plasma protein S; Vitamin K-dependent protein S
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	ANSLLEETKQGNLERECIEELCNKEEAREVFENDPETDYFYPKYLVCLRSFQTGLFTAARQSTNAYPDLRSCVNAIPDQCSPLPCNEDGYMSCKDGKASFTCTCKPGWQGEKCEFDINECKDPSNINGGCSQICDNTPGSYHCSCKNGFVMLSNKKDCKDVDECSLKPSICGTAVCKNIPGDFECECPEGYRYNLKSKSCEDIDECSENMCAQLCVNYPGGYTCYCDGKKGFKLAQDQKSCEVVSVCLPLNLDTKYELLYLAEQFAGVVLYLKFRLPEISRFSAEFDFRTYDSEGVILYAESIDHSAWLLIALRGGKIEVQLKNEHTSKITTGGDVINNGLWNMVSVEELEHSISIKIAKEAVMDINKPGPLFKPENGLLETKVYFAGFPRKVESELIKPINPRLDGCIRSWNLMKQGASGIKEIIQEKQNKHCLVTVEKGSYYPGSGIAQFHIDYNNVSSAEGWHVNVTLNIRPSTGTGVMLALVSGNNTVPFAVSLVDSTSEKSQDILLSVENTVIYRIQALSLCSDQQSHLEFRVNRNNLELSTPLKIETISHEDLQRQLAVLDKAMKAKVATYLGGLPDVPFSATPVNAFYNGCMEVNINGVQLDLDEAISKHNDIRAHSCPSVWKKTKNS
Expression Range	42-676aa
Protein Length	Full Length of Mature Protein
Mol. Weight	86.6kDa
Research Area	Cardiovascular
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.
Subcellular Location	Secreted.
Database References	HGNC: 9456 OMIM: 176880 KEGG: hsa:5627 STRING: 9606.ENSP00000377783 UniGene: PMID: 28374852 Functional PROS1 variants are more common in the general Swedish population than anticipated. PMID: 29883906 The present case combined with the review of the literature suggests that p.Arg451* in the PROS1 gene mainly leads to clinically evident thrombosis following trauma, surgery or serious comorbidities especially malignancy. PMID: 29321366 Protein S and Gas6 mediates phagocytosis of HIV-1-infected cells by bridging receptor tyrosine kinase Mer to phosphatidylserine exposed on infected cells. PMID: 29304470 these results suggest a novel pathogenic role of SPE B that initiates protein S degradation followed by the inhibition of apoptotic cell clearance by macrophages PMID: 27181595 Taken together, our gain-of-function, loss-of-function analyses suggest that PROS may facilitate cell proliferation and promote castration resistance in human castration-resistant PCa-like cells via its apoptosis-regulating property. PMID: 27342144 protein S has a clinical relevance and a protective role in pulmonary fibrosis PMID: 27172994 Due to the versatility exemplified, this probe holds great promise for exploring the role of protein S-nitrosylation in the pathophysiological process of a variety of vascular diseases PMID: 28284075 There was no association between PS-Tokushima and recurrent pregnancy loss and a PS deficiency or low PS activity was shown not to serve as a reliable clinical predictor of subsequent miscarriage. PMID: 28214760 we identify PROS1 as a driver of Oral Squamous Cell Carcinoma tumor growth and a modulator of AXL expression PMID: 28118606 The prevalence of PS de fi ciency in the present study was higher than in Western countries and con fi rms the high prevalence of PS de fi ciency in Asian populations PMID: 27748013 Patients with type 2 diabetes had significantly lower circulating free protein S than healthy control subjects PMID: 27207541 In the present study, gene analysis of six unrelated Japanese families diagnosed with congenital protein S deficiency identified five missense mutations in the PROS1 gene - c.757C>T (Ala139Val; A139V), c.1346 G>T (Cys449Phe; C449F), c.1352G>A (Arg451Gln; R451Q), c.1424G>T (Cys475Phe; C475F) and c.1574C>T (Ala525Val; A525V) - and one frameshift mutation, c.2135delA (Asp599ThrfsTer13; D599TfsTer13). PMID: 28088608 The odds ratio of developing idiopathic fatal pulmonary embolism as a variant carrier for PROS1 is 56.4 (95% CI, 5.3-351.1; P = 0.001). PMID: 28174134 described a novel PROS1 frameshift mutation, c.74dupA, in a hereditary protein S deficiency family. Interestingly, both of the proband and his mother carried the mutation and had a protein S deficiency, however, only the proband suffered a pulmonary embolism while his mother had no history of any thrombosis, suggesting that a triggering event might have been involved in the thrombus formation. PMID: 27846449 PROS1 may play an important role in the development of glioblastoma multiforme through cellular proliferation, migration and invasion as well as apoptosis. PMID: 27840905 Low protein S expression is associated with diabetes mellitus. PMID: 27345772 analysis of genotype phenotype correlation in PROS1 in a large cohort of adults with suspicion of inherited quantitative protein S deficiency PMID: 26466767 The immunoabsorption of PON1 from plasma significantly reduced protein S anti-coagulant activity. PMID: 26358807 Elevated levels of circulating microparticles can play a role in carriers of mild and severe inherited thrombophilia resulting from protein S deficiency. PMID: 26354831 The ELISA system using the PS K196E mutation-specific antibody is a useful tool for the rapid identification of PS K196E carriers, who are at a higher risk for venous thromboembolism. PMID: 26186226 A PROS1 c.1486_1490delGATTA mutation on exon 12. appeared to be the primary cause of thrombosis in the family of the present study. PMID: 25997409 analysis of the amino acid residues in the laminin G domains of protein S involved in tissue factor pathway inhibitor interaction PMID: 25716664 Women with low levels of plasma PS activity and free PS during early pregnancy might have increased risks of pregnancy-induced hypertension, or pre-eclampsia. PMID: 25879167 analysis of compound heterozygote protein S deficiency in two Japanese patients [case reports] PMID: 25868595 Protein S exacerbates acute alcoholic hepatitis by inhibiting apoptosis of activated natural killer T cells. PMID: 25399514 The present study highlights that the GAS6/ProS-TAM system correlates in several ways with disease activity in systemic lupus erythematosus PMID: 23497733 genetic polymorphism affects endogenous thrombin potential among FV Leiden carriers PMID: 24226152 Identical large duplication mutation of PROS1 was detected in 3 unrelated patients with thrombophilia from hereditary protein S deficiency. PMID: 24992033 Anti-human protein S antibody induces tissue factor expression through a direct interaction with PFKP and ERK1/2 activation in coronary artery endothelial cells. PMID: 24331211 Data indicate that the protein S sex hormone-binding globulin (SHBG)-like domain was important for binding and enhancement of tissue factor pathway inhibitor (TFPI). PMID: 24740810 study found the S K196E mutation, a genetic risk factor for venous thromboembolism, was not found in Chinese or Koreans; results suggest the protein S K196E mutation is a recent occurrence and fixed within the Japanese population PMID: 23721692 implicate protein S residues 37-50 as a binding site for FVa that mediates, at least in part, the direct inhibition of FVa-dependent procoagulant activity by protein S PMID: 23892573 miR-494 is involved in the mechanism of estrogen-mediated downregulation of PS expression PMID: 23789915 This is the first report of a large deletion of PROS1 from exon 1 through 12 in Polish patients with deep-vein thrombosis. PMID: 23473639 PS exerts anticoagulant cofactor activity with TFPIalpha from any physiological pool, likely by localizing TFPIalpha to membrane surfaces, stabilizing its interaction with membrane-bound FXa, and slowing thrombin generation. PMID: 24233490 Data suggest that protein S testing and PROS1 testing should not be considered in unselected patients with venous thrombosis. PMID: 24014240 This study is the largest investigation of ProS deficiency in China and the first investigation of the influence of Type I ProS missense mutations on the global level of coagulation function. PMID: 23813890 The effect of oral contraceptives on TFPI and PS is a possible explanation for the increased risk of venous thrombosis associated with oral contraceptives. PMID: 23407778 Data indicate that activated T cells express Pros1. PMID: 23850380 Platelet protein S may be an essential pool of protein S that counterbalances procoagulant activities on platelets. PMID: 23238804 sputum glutaredoxin-1 may have a role in asthma, while protein S may have a role in better lung function PMID: 23370801 The tyrosine kinase receptor MER is activated by PROS and mediates its inhibitory effect on VEGF-A-induced EC proliferation. PMID: 23065156 TFPI Kunitz domain 3 residue Glu226 is essential for TFPI enhancement by protein S. PMID: 23074276 PROS1 is elevated in high grade and castration-resistant prostate cancer and could serve as a potential biomarker of aggressive disease. PMID: 22908226 mutation deletions but no other types of rearrangements detected in patients with protein S deficiency PMID: 22627709 genetic polymorphism is associated with increased free plasma tissue factor pathway inhibitor levels PMID: 22273984 proteolytic cleavage is increased in patients with essential thrombocythaemia and reduced in patients with chemotherapy-induced thrombocytopenia PMID: 22318644 Serum of women during third trimester of pregnancy complicated by intrauterine growth restriction (IUGR) had significantly lower functional and free protein S compared with control; however, no correlation was found between total protein S and IUGR. PMID: 22104477 Persistently low protein S activity levels are highly indicative of a genetic alteration in PROS1. PMID: 22261441

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

📧 inquiry@betalifesci.com 📞 +1(201) 888-7983

Contact Us Now for Inquiries, Orders, and Questions

Recombinant Human Vitamin K-Dependent Protein S (PROS1) Protein (His-SUMO)

Recombinant Human Vitamin K-Dependent Protein S (PROS1) Protein (His-SUMO)

Product Overview

Target Details

FAQs