Recombinant Human VCAM-1 Protein (C-6His)

Name: Recombinant Human VCAM-1 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-0392NP
Availability: InStock

BL-0392NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Vascular Cell Adhesion Protein 1 is produced by our Mammalian expression system and the target gene encoding Phe25-Glu698 is expressed with a 6His tag at the C-terminus.
Accession	P19320
Synonym	Vascular Cell Adhesion Protein 1; V-CAM 1; VCAM-1; INCAM-100; CD106; VCAM1; L1CAM
Gene Background	VCAM-1 is a single-pass type I membrane protein, contains 7 Ig-like C2-type domains. It is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.
Molecular Mass	75.26 KDa
Apmol Mass	85-120 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 2mM CaCl2, 2mM MgCl2, 5% Threhalose, pH 7.2.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Database References	HGNC: 12663 OMIM: 192225 KEGG: hsa:7412 STRING: 9606.ENSP00000294728 UniGene: PMID: 28496046 not a specific screening marker for pulmonary arterial hypertension in systemic sclerosis PMID: 29687288 We observed an increase of VCAM-1 urine levels in diabetic nephropathy-basal patients compared to diabetic controls and an increase of urinary neprilysin in DN-treated patients with persistent albuminuria PMID: 29854824 Urinary angiostatin and VCAM-1 are predictive of specific histological changes in concurrent lupus nephritis renal biopsies. PMID: 29076253 In summary, it could be concluded that the expression of gene VCAM-1 was down regulated by taurocholic acid through miR-590-3p and that gene VCAM-1 could participate in the regulation of cell growth, cell proliferation and cell apoptosis PMID: 28851649 VCAM-1 expression is upregulated by CD34+/CD133+-stem cells derived from septic patients PMID: 29601599 Circulating soluble vascular cell adhesion molecule (VCAM-1) level was a useful diagnostic parameter for understanding the difference between coronary artery disease (CAD) and cardiac syndrome X (CSX). PMID: 28675064 findings indicate a novel role for JAK2-V617F in activation of beta1 integrins and enhanced adhesion of granulocytes and 32D myeloid progenitors to VCAM1-coated surfaces PMID: 28096537 High VCAM1 expression is associated with Cardiovascular Disease Risk. PMID: 28202840 VCAM-1 levels are elevated in serum of cystic fibrosis patients. PMID: 28646244 VCAM-1 levels are able to accurately predict significant fibrosis in non-alcoholic fatty liver disease patients PMID: 28461687 These results revealed the effect of CD106 and NF-kappaB in bone marrow failure of Acquired aplastic anemia. PMID: 28764810 Overexpression of LINC00341 suppresses the expression of vascular cell adhesion molecule 1 (VCAM1) and the adhesion of monocytes induced by atheroprone flow and tumor necrosis factor-alpha. PMID: 28500253 Increased expression of PECAM-1, ICAM-3, and VCAM-1 in colonic biopsies from patients with inflammatory bowel disease (IBD) in clinical remission is associated with subsequent flares; this suggests that increases in the expression of these proteins may be early events that lead to flares in patients with IBD PMID: 27552332 Association with the efficiency of hematopoietic stem cells (HSC) mobilization to PB were found in the SNPs of the DGKB gene involved in cell transport and SDF-1-induced migration ability and of the VCAM1 gene which is essential for HSC homing, suggesting that SNPs involved in cell migration ability might be partly involved in HSC PMID: 27839966 sVCAM-1 reflects xerostomia in primary Sjogren's syndrome. sICAM-1 and sE-selectin may be additional parameters of secondary Sjogren's syndrome activity. PMID: 29068581 Augmented expression of endothelial adhesion molecules ICAM1/VCAM1 is involved in the pathophysiology of patients with antiphospholipid syndrome. PMID: 29096830 VCAM-1 knockdown reduced TNFalpha-induced HUVEC migration and tube formation. PMID: 28209985 High levels of serum ICAM-1 was associated with the development of multiple organ failure . High levels of VCAM-1 was associated with both multiple organ failure and in-hospital mortality. PMID: 27701021 VCAM1 protein expression increased in cartilage tissue from spinal tuberculosis patients. PMID: 28829887 This study provided direct evidence that increasing extracellular Ca(2+) enhanced TNF-alpha-induced VCAM-1 activation and monocytes adhesion. Moreover, we identified a novel TRPC1/ERK1/2/NFkappaB signaling pathway mediating VCAM-1 activation and monocyte adhesion in this pathological process. PMID: 28583863 Findings suggest that sVCAM-1 is related to affective temperaments, and it is a trait marker for liability to mood disorders. This relationship between alterations in cellular adhesion and affective temperament may be important for vulnerability to affective disorders. PMID: 27693464 patients with newly-diagnosed MM have increased serum levels of VCAM-1 and ICAM-1 that correlate with advanced disease features. VCAM-1 is also independently correlated with survival, suggesting an important role in the biology of the disease and supporting its targeting with novel anti-myeloma drugs. PMID: 27232930 VCAM-1 was statistically elevated in major depressive disorder. PMID: 27598970 Data show that podoplanin (PDPN), CD106 (VCAM-1) and CD248 protein were increased in diseased compared to healthy tendon cells. PMID: 28122639 Peptide nanoparticles targeting VCAM-1 are suggested as a theranostic carrier for drug delivery to inflamed endothelium. PMID: 27105996 IRF-5 targeted the expression of vascular cell adhesion molecule 1 (VCAM-1) at the transcriptional level by binding to its promoter. PMID: 28818665 In patients with optic neuritis that developed into multiple sclerosis, there was a decrease in serum VCAM-1. PMID: 27806869 There was a negative correlation of miR-126 with the PCI-induced markers of inflammation such as hs-CRP and VCAM-1 PMID: 27900989 this study shows that quercetin reduces the expression of VCAM-1 in oxidized LDL cholesterol-induced endothelial cells PMID: 27585526 Gastrin-releasing peptide (GRP) increased the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) by activating nuclear factor-kappaB (NF-kappaB) in endothelial cells. PMID: 28093230 Mesothelium VCAM-1 expression is negatively associated with progression-free and overall survival in EOC. This is especially compelling in light of previous data suggesting that persistent VCAM-1 expression during treatment is an indicator of platinum resistance. PMID: 28263384 The results of this study suggest that VCAM-1 gene variants may be related to obesity and inflammatory markers in the Korean population PMID: 27853845 Panax notoginseng saponins and Ginsenoside protect aorta form atherosclerosis via Rb1 via activation of Nrf2 and suppression of p38 - VCAM-1 signal pathway. PMID: 27620662 Our results suggest that an impairment in adhesion exists in vitiligo skin, which is supported by the diminished immunohistochemical expression of laminin, beta1 integrin, ICAM-1 and VCAM-1. PMID: 28214208 Th1-induced CD106 expression mediates leukocytes adhesion on synovial fibroblasts from juvenile idiopathic arthritis patients via TNF-alpha. PMID: 27123929 Although the expression of VCAM-1 was higher in adult T-cell leukemia/lymphoma patients individuals, there was no significant difference between the studied groups. PMID: 28110427 Activin A and preeclamptic serum upregulate ET-1, ICAM-1, and VCAM-1 in human umbilical vein endothelial cells, consistent with activin A contributing to the pathophysiology of preeclampsia. PMID: 27155341 Statistically significant associations were identified between the chronic periodontal patients and the controls in the gene polymorphisms of rs5498 ICAM-1 (P=0.007) and rs1041163 VCAM-1 (P=0.029). The distribution of rs5498 (P=0.029) and rs1041163 (P=0.049) differed significantly across the mild, moderate, and severe groups of periodontitis. PMID: 27391418 VCAM-1 had a synergistic effect with coronary artery disease on the BDNF PMID: 28131673 findings suggest that the increased levels of IL-17,Hcy and sVCAM contributes contributes to the vascular inflammation and activation presented by sickle cell anemia patients PMID: 26334689 VCAM-1 plays an important role in the development and generation of gestational hypertension. PMID: 27706617 gLDL stimulate the expression of cellular VCAM-1, the secretion of soluble VCAM-1, and the adhesion of monocytes through mechanisms involving p38 MAP kinase and NF-kB signalling pathways activated by RAGE, ERS and oxidative stress, thus contributing to diabetic atherosclerosis. PMID: 27206739 High VCAM1 expression is associated with non-insulin resistant women with polycystic ovary syndrome. PMID: 27245110 Denbinobin inhibited IL-1beta-induced ICAM-1/VCAM-1 expression and monocyte adhesion to osteoarthritis fibroblast-like synoviocytes via mirn146-a PMID: 25052989 Theobroma cacao extract prohibits the increase in IL-6 and sVCAM-1 in endothelial cells induced by plasma from preeclamptic patients. PMID: 26955771 BD patients have a lower expression of ICAM-1, VCAM-1, and E-selectin in their tumor tissues. BD-HDL facilitates the adhesion of tumor cells to vascular endothelium by upregulating the expression of ICAM-1 and VCAM-1, thereby promoting the initial progression of breast cancer metastasis PMID: 26872904 Clopidogrel diminishes TNFalpha-stimulated VCAM-1 expression at least in part via HO-1 induction and CaMKKbeta/AMPK/Nrf2 pathway in endothelial cells. PMID: 26824050 Results show that VCAM1 expression is regulated by TSPO to activate endothelial cells. PMID: 26608360 Human placental, chorionic villi-derived mesenchymal stem cells expressing VCAM-1 promote angiogenesis in a mouse ischemia model. PMID: 27044487

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

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