Recombinant Human Urocortin-2 (UCN2) Protein (His-SUMO)

Name: Recombinant Human Urocortin-2 (UCN2) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-06430P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Urocortin-2 (UCN2) Protein (His-SUMO) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q96RP3
Target Symbol	UCN2
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	IVLSLDVPIGLLQILLEQARARAAREQATTNARILARVGHC
Expression Range	72-112aa
Protein Length	Partial
Mol. Weight	17.4 kDa
Research Area	Cardiovascular
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Suppresses food intake, delays gastric emptying and decreases heat-induced edema. Might represent an endogenous ligand for maintaining homeostasis after stress.
Subcellular Location	Secreted.
Protein Families	Sauvagine/corticotropin-releasing factor/urotensin I family
Database References	HGNC: 18414 OMIM: 605902 KEGG: hsa:90226 STRING: 9606.ENSP00000273610 UniGene: PMID: 29499655 examination of the local and systemic cardiovascular effects of urocortin 2 and urocortin 3 in healthy subjects and patients with heart failure PMID: 27275843 Data show that corticotropin releasing hormone receptor 2 (Crhr2) protein is abundantly expressed in the heart, and plasma Ucn2 levels were higher in patients with heart failure compared to healthy controls, indicating that endogenous Crhr2 is associated with heart failure. PMID: 28550160 up-regulated in placenta, fetal membranes, and myometrium in labor PMID: 25426872 Corticotrophin-Releasing Factor (CRF) and the urocortins are potent regulators of the inflammatory phenotype of human and mouse white adipocytes. PMID: 24835211 Stresscopin-related peptide induces a weaker enhancement of cardiovascular function through corticotropin-releasing factor receptorn 2 than that induced by stresscopin. PMID: 23850799 Solubilized SUMO-eXact-preproUCN2 was used successfully to generate two high affinity mouse monoclonal antibodies specific to the pro-region of urocortin 2. PMID: 24291344 UCN2 is significantly associated with AAA and inhibits VSMC proliferation by inducing a G1 cell cycle arrest suggesting a plausible regulatory role in AAA pathogenesis PMID: 24107226 UCNII caused cell death via different membrane-disrupting mechanisms that involve aggregation & membrane depolarization in bacteria and pore formation in Leishmania even inside macrophages. Innate immune cells produce UCNII in response to infections. PMID: 24249730 Description of ucn2 posttranslational processing and peptide sequence. Hypoxia and glycosylation, paradigms that might influence secretion or processing of gene products, did not significantly impact hUcn 2 prohormone cleavage. PMID: 23493376 study showed that Ucn2 and Ucn3 differentially regulate the LPS-induced TNF-alpha and IL-10 expression and secretion in trophoblast explants acting through CRH-R2. Ucn2 is proinflammatory. PMID: 22000474 These data suggest a possible involvement of urocortin 2 and Ucn 3 in the mechanisms of endometriosis. PMID: 21454316 Confirm the anti-inflammatory function of VIP, through the modulation of the expression of CRF system that impacts in a reduction of mediators with inflammatory/destructive functions. PMID: 21360527 Unlike urocortin-1 levels, higher levels of urocortin 2 are not associated with worse left ventricular diastolic performance in patients with chronic systolic heart failure. PMID: 20670842 Urocortin 2 may play a role in the regulation of estradiol production throughout pregnancy, thereby contributing to the placental regulation of key reproductive events in pregnancy maintenance and parturition. PMID: 19417223 Placental Ucn2 and Ucn3 expression is sensitive to O(2) tensions and mediated by HIF-1alpha. In preeclampsia, the increased expression of both peptides may reflect a response to the oxidative stress. PMID: 20616367 SCP and UCN are potent activators of the p42/44 MAPK pathway, with SRP able to induce phosphorylation of p42/44 MAPK as well, albeit not as pronounced PMID: 14519439 data suggest a distinctive role for corticotropin-releasing hormone receptor 2 specific agonists like urocortin II in the control of myometrial contractility during human pregnancy PMID: 14592950 data indicate that intracisternal Ucn 2 induced a central CRF(2)-mediated inhibition of gastric emptying involving sympathetic alpha(1)-adrenergic mechanisms independent from the vagus contrasting with the vagal-dependent inhibitory actions of CRF & Ucn 1 PMID: 16223946 the 38 amino acid urocortin 2 peptide is the minimal pharmacophore PMID: 16476507 UCN2 and UCN3 are expressed in human placenta, decidua, and fetal membranes and may be proposed as regulators of placental vascular endothelial tone. PMID: 16626608 Both peptides were immunolocalized in epithelial, stromal, and endothelial cells. PMID: 17074339

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.