Recombinant Human Upstream Stimulatory Factor 1 (USF1) Protein (His)

Name: Recombinant Human Upstream Stimulatory Factor 1 (USF1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-10061P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Upstream Stimulatory Factor 1 (USF1) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P22415
Target Symbol	USF1
Synonyms	USF1; BHLHB11; USF; Upstream stimulatory factor 1; Class B basic helix-loop-helix protein 11; bHLHb11; Major late transcription factor 1
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	GQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN
Expression Range	3-310aa
Protein Length	Partial
Mol. Weight	37.3kDa
Research Area	Transcription
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Transcription factor that binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') that is found in a variety of viral and cellular promoters.
Subcellular Location	Nucleus.
Database References	HGNC: 12593 OMIM: 144250 KEGG: hsa:7391 STRING: 9606.ENSP00000356999 UniGene: Hs.414880
Associated Diseases	Hyperlipidemia combined 1 (HYPLIP1)

Gene Functions References

Study did not show any associations between rs2516839 and rs3737787 polymorphisms of the USF-1 gene and pediatric ischemic stroke. However, found an influence of specific genotypes on the outcome of stroke, including epilepsy, dysarthria, and a decrease in intellectual functioning. PMID: 29598907
our study first demonstrated that USF1 could activate the transcription of MUC13, thereby enhancing the proliferation and self-renewal of glioblastoma stem cells PMID: 29441861
miR-483 is a self-regulating microRNA and can activate its own expression via USF1 PMID: 27693430
Findings suggest the underlying mechanism of upstream transcription factor 1 (USF1) in papillary thyroid cancer (PTC) development. PMID: 26052935
SNP (single-nucleotide polymorphism) alleles that reduced USF1 mRNA expression also displayed a beneficial cardiometabolic profile, featuring improved insulin sensitivity, a favorable lipid profile, and reduced atherosclerosis PMID: 26819196
USF1 gene can take part in basal transcription regulation of the human A3G gene in hepatocyte, and the identified E-box represented a binding site for the USF1. PMID: 26772882
A trend of increasing triglycerides levels in relation to the C allele dose of rs2516839 SNP was observed. The synergistic effect of cigarette smoking and C allele carrier state on CAD risk was found. PMID: 26068452
Rta autoregulates BRLF1 transcription via its interaction with USF1 on E1. PMID: 26297580
Results show that the USF1 and APOA5 polymorphisms are associated with Familial combined hyperlipidemia and that the S19W SNP in the APOA5 gene is associated to the disease independently of total cholesterol, triglycerides and body mass index. PMID: 25308402
The carriers of minor alleles of rs2516839 (C) and rs1556259 (G) of USF1 gene had decreased USF1 expression in atherosclerotic plaques as compared to non-carriers. PMID: 24722012
Our results provided a new insight into susceptibility of USF1 variant with male infertility. rs11204546 of OR2W3 and rs11677854 of GTF2A1L might be additional valuable molecular predictive markers PMID: 25374392
CK2 phosphorylation of USF1 modulates two functionally important properties of USF1, namely hetero-dimerization and transactivation. PMID: 25194820
Further studies may be needed to validate our results and gain insights into the pathological mechanism of USF1 gene in the HCC tumorigenesis. PMID: 25480412
A significantly elevated genotype frequency in certain USF1 genes was observed in hepatocellular carcinoma patients. PMID: 25367853
Increased upstream stimulatory factor in HCC is associated with heparanase expression. USF might be an important factor in regulating HPSE expression and act as a novel marker of metastatic recurrence of HCC patients PMID: 25149140
They underscore the new role of USF1 and give new clues of how p53 loss of function can occur in any cell type. PMID: 24831529
USF1 binding to an E-box element may be critical for basal MVP promoter activation. PMID: 24173679
Knockdown of USF-1 protected the HepG2 cells from oxygen and glucose deprivation (OGD)-induced apoptosis. PMID: 24661879
The rs2516838 and rs2516839 polymorphisms of USF1 influence total unstable CPA in atherosclerotic stroke patients, which might be new markers to predict the risk of recurrence for this disease. PMID: 23271305
USF1 SNP's distribution and genetic risk for cardiovascular disease in Azoreans (Portugal) PMID: 24042129
our findings reveal that the guided-recruitment of the hSET1A histone methyltransferase complex and its H3K4 methyltransferase activity by transcription regulator USF1 safeguards hematopoietic transcription programs PMID: 23754954
These results suggest USF1 has an independent but gender- and age-associated effect on Alzheimer Disease-related brain lesion development. PMID: 22390463
USF1 and USF2 are important regulatory factors for Alx3 gene expression in different cell types PMID: 23181698
Transcription factors upstream stimulatory factor 1 (USF-1) and USF-2 are indispensable for the transcription of RORgammaT in human lymphocytes. PMID: 22891280
Identify transcriptional circuitry whereby USF-1 variants influence hepatic triglyceride secretion in familial combined hyperlipidemia and related disorders. PMID: 22460558
the loss of USF-1 compromises DNA repair, which suggests that USF-1 plays an important role in maintaining genomic stability. PMID: 22291606
the T-1514C polymorphism affects TBX21 gene expression and Th1 cytokine production by binding USF-1 to the SNP site PMID: 22258560
The E-box binding factors Max/Mnt, MITF, and USF1 act coordinately with FoxO to regulate expression of proapoptotic and cell cycle control genes by phosphatidylinositol 3-kinase/Akt/glycogen synthase kinase 3 signaling. PMID: 21873430
These data indicate that an E-box motif (RBE1) within the core promoter in the long terminal repeat of HIV-1 is a bona fide binding site for the RBF-2 transcription factor complex USF1, USF2, and TFII-I. PMID: 21813151
This study demonstrated the involvement of USF1 and USF2 as molecular targets of Helicobacter pylori and the key role of DNA methylation in their regulation. PMID: 20180799
USF1 is expressed in the nuclei of nicotine-stimulated odontoblasts, but in the cytoplasm of unstimulated odontoblasts. PMID: 18070436
Upstream stimulatory factor 1 could regulate the osteopontin expression in odontoblasts. PMID: 18307922
there is an indication of an interaction between APOA5 and USF1 on the risk for metabolic syndrome PMID: 20054229
Over-expression of human USF1 in transgenic mice influenced metabolic traits. PMID: 19995791
Defective gene induction of USF1 variant by insulin contributes to the risk of developing dyslipidemia. PMID: 20031629
Reinforce the roles of APOA5 and USF1 variants on cardiovascular phenotypes and suggest that both genes contribute to lipid levels and aortic atherosclerosis individually and possibly through epistatic effects. PMID: 19910639
requirement of carboxy ester lipase gene E-box for activation binds upstream regulatory factor 1 and 2 PMID: 11945176
Cooperative E-box regulation of human GLI1 by TWIST and this protein PMID: 11948912
demonstrated a novel mechanism for USF activation, which contributes to differential vasopressin expression in lung cancer. PMID: 12403649
data indicated that upstream-stimulatory-factor (USF) might be involved in the basal transcriptional machinery of apolipoprotein E by binding to a non-canonical E-box motif within the proximal promoter PMID: 12444925
transfection of expression vectors containing USF-1 or USF-2 cDNAs activated CYP1A2 reporter gene activity, while a dominant-negative USF-2 expression vector blocked such activity. PMID: 12663044
we report the molecular cloning and functional characterization of a novel alternative splicing variant of human USF1 (hUSF1), termed USF1/BD. PMID: 12851711
These findings suggest that increased protein levels and DNA binding of USF1 and USF2 mediate the inhibitory effects of hypoxia and of Mash-2 on CYP19 gene expression in human placenta PMID: 12917334
USF1 transcription is regulated by cha, a basic helix-loop-helix transcription factor PMID: 12923186
USF1/2 activates the hTERT promoter exclusively in hTERT-positive cells in a manner that is enhanced by the coactivator p300 and attenuated upon inhibiting p38-MAP kinase, a known modulator of USF activity. PMID: 12970752
USF and Sp1 proteins interact and regulate human deoxycytidine kinase promoter activity PMID: 14514691
transcription factors upstream stimulatory factor (USF) and c-Myc may exert opposing effects on PIGR promoter activity PMID: 14644095
encodes a transcription factor known to regulate several genes of glucose and lipid metabolism PMID: 14991056
role in regulating transcription of many genes involved in lipid and glucose homeostasis PMID: 15054483
the influencing feature of both glucose and lipid homeostasis showing case-control heterogeneity PMID: 15175273

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.