Recombinant Human Translationally-Controlled Tumor Protein (TPT1) Protein (His)

Name: Recombinant Human Translationally-Controlled Tumor Protein (TPT1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-06410P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Translationally-Controlled Tumor Protein (TPT1) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P13693
Target Symbol	TPT1
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC
Expression Range	1-172aa
Protein Length	Full Length
Mol. Weight	23.7 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Involved in calcium binding and microtubule stabilization.
Subcellular Location	Cytoplasm.
Protein Families	TCTP family
Database References	HGNC: 12022 OMIM: 600763 KEGG: hsa:7178 STRING: 9606.ENSP00000431872 UniGene: PMID: 30066846 The biological effects of Na,K-ATPase and TCTP interaction are reviewed. PMID: 29867020 The cells knocked down for TCTP show diminished invasiveness and migration under TGF-beta1 treatment. TGF-beta1 dependent TCTP expression is required for epithelial to mesenchymal transition in cells. PMID: 28958626 that TPT1 knockdown altered the BECN1 interactome, a representative MTOR-independent pathway, to stimulate autophagosome formation PMID: 28409693 Transfection with TCTP-Lipid modified antisense oligonucleotides led to rapid and prolonged internalization via macropinocytosis, TCTP downregulation and significant decreased cell viability in in castration-resistant prostate cancer cells. PMID: 28472637 extracellular TCTP has a role as a promoter of colorectal cancer progression and liver metastases via Cdc42/JNK/MMP9 activation PMID: 27367023 The structure of human HRF (hHRF) was solved in 2 new crystal forms, one at the highest resolution (1.4A) yet reported. The high resolution hHRF structure reveals a disulphide-linked dimer, in which the 2 molecules are closely associated and provides a model for the role of HRF in mast cell activation. PMID: 29216544 analyzed the interactome of TCTP in HeLa cells by coimmunoprecipitation (IP) and mass spectrometry (MS). Results not only reveal a large number of TCTP-associated proteins that possess pleiotropic functions, but also provide novel insights into the molecular mechanisms of TCTP in tumorigenesis PMID: 27607350 Fortilin directly interacts with the cytoplasmic domain of IRE1alpha, inhibits both kinase and endoribonuclease (RNase) activities of this stress sensor, and protects cells against apoptotic cell death at both cellular and whole animal levels. PMID: 28550308 These findings provide novel data on the role and regulation of TCTP in skeletal muscle in vivo. PMID: 27813490 TCTP may thus contribute to the development of anti-cancer drug resistance. PMID: 28143584 The findings suggest that the synergistic actions of Histamine Releasing Factor and Histamine Releasing Factor-reactive IgE may play important roles in the pathogenesis of Chronic Urticaria. PMID: 28219065 Downregulation of the expression of TCTP inhibits the migration and invasion of glioma cells. PMID: 26328748 These data may suggest an involvement of translationally controlled tumor protein in normal physiology and carcinogenesis. PMID: 26425551 High HRF expression is associated with non-Hodgkin lymphomas. PMID: 25363345 Results demonstrate that TCTP mRNA translation is regulated by signalling through the PI3-K/Akt/mTORC1 pathway in neoplasms. PMID: 25936523 High phospho-TCTP expression levels are associated with aggressive breast cancer. PMID: 25779659 TCTP binds to hemin and functions as a "buffer protein", controlling cellular homeostasis. PMID: 25396429 analysis of how TCTP-PTD penetrates A549 human lung cancer cell membranes and promotes protein internalization PMID: 25423047 Data indicate that Tanshinone IIA (Tan-IIA)T upregulated the protein expression of CHOP and Bax, but downregulated the protein expression of BiP, TCTP, Mcl-1 and Bcl-xL. PMID: 25270224 Targeting Hsp27 interaction with TCTP, a partner protein identified in a genetic screen, increases therapy sensitivity, and could decrease or abolish toxicity to normal cells. PMID: 25277244 TCTP is functionally implicated in the progression of NF1-associated tumors. PMID: 25092287 osteoclastogenic activity of translationally-controlled tumor protein (TCTP) with reciprocal repression of p21 PMID: 25263704 Interaction of translationally controlled tumor protein with Apaf-1 is involved in the development of chemoresistance in HeLa cells. PMID: 24606760 High TCTP expression is associated with glioma. PMID: 24311645 Plasmodium falciparum TCTP showed a lower B cell proliferative effect than human TCTP. PMID: 24465583 Cofilin-1 expression positively correlates with the tumor grade, and p23 expression correlates with the endoplasmic reticulum positive status. PMID: 24106833 TCTP is a novel mediator of endothelial prosurvival and growth in patients with pulmonary arterial hypertension. PMID: 24657995 observations indicate that TCTP enhances Pim-3 stability to simultaneously promote and prevent cell-cycle progression and apoptosis, respectively PMID: 24165482 TCTP may have a role in prostate cancer development PMID: 23894469 Data indicate that translationally controlled tumor protein (TCTP) expression in glioma was an independent prognostic factor, which might be a potential diagnostic and therapeutic target of glioma. PMID: 23749504 TCTP binds to the beta domain of VHL through competition with HIF1alpha, which promotes VHL degradation by the ubiquitin-proteasome system and HIF1alpha stability. PMID: 23387829 human miR-27b regulates the expression of the TCTP tumor protein, and circulating miR-27b may be useful as a biomarker for oral cancer research. PMID: 22902387 Demonstrate the presence of TCTP in human cornea, and suggest a potential role in the pathogenesis of herpes virus keratitis. PMID: 22853445 Results imply that TCTP might be a mediator of PRL-3-promoted proliferation, migration and invasion of human colon cancer cells. PMID: 22340241 Data suggest that TCTP may play a critical role in maintaining genomic integrity in response to DNA-damaging agents. PMID: 22451927 CHD1L/TCTP/Cdc25C/Cdk1 molecular pathway causes the malignant transformation of hepatocytes with the phenotypes of accelerated mitotic progression and the production of aneuploidy PMID: 21953552 The TCTP is a survival factor that protects cancer cells from oxidative stress-induced cell-death. PMID: 21801721 These results identify TCTP-bearing nanovesicles as a novel component of the paracrine apoptotic program of potential importance in vascular repair. PMID: 20966960 The Chfr-TCTP interaction was stable throughout the cell cycle, but it could be diminished by the complete depolymerization of the microtubules. PMID: 18504434 Knockdown of TCTP inhibited proliferation, migration, and invasion activities of LoVo cells in vitro and in vivo. PMID: 19621893 Data suggest a kind of feedback regulation between TCTP and VDR to regulate a variety of (Ca(2+) dependent) cellular effects and in this way further underscore the physiological relevance of the observed physical interaction beteen TCTP and VDR. PMID: 19815065 The mRNA of the translationally controlled tumor protein P23/TCTP is a highly structured RNA, which activates the dsRNA-dependent protein kinase PKR (TCTP PROTEIN) PMID: 11991642 Physical and functional interaction between myeloid cell leukemia 1 protein (MCL1) and this protein PMID: 12149273 Human TCTP was identified as a putative Plk-interacting clone by a two-hybrid screen. Plk phosphorylates TCTP on two serine residues in vitro and cofractionates with the majority of kinase activity toward TCTP in mitotic cell lysates. PMID: 12167714 genetic regulation in tumor reversion PMID: 12399545 results do not support the hypothesis that IgE(mn+) induces a late allergic reaction (LAR), but do not exclude the alternative hypothesis that HRFs are released during a LAR and contribute to asthma severity PMID: 12487227 Patients with Multiple drug allergy syndrome and more than 1/3 with hypersensitivity to a single antibacterial drug showed the presence of circulating histamine-releasing factors. Such factors might play a role in drug-induced adverse reactions. PMID: 12876410 HRF p23 can stimulate nonimmune epithelium such as bronchial epithelium PMID: 12948934 Human recombinant histamine-releasing factor can partially inhibit the secretion of IL-2 and IL-13 protein in a dose-dependent manner from both purified peripheral blood T cells and Jurkat T cells. PMID: 14500674

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.