Recombinant Human Transcription Factor Sox-2 (SOX2) Protein (His)

Name: Recombinant Human Transcription Factor Sox-2 (SOX2) Protein (His)
Brand: Beta LifeScience
SKU: BLC-06361P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Transcription Factor Sox-2 (SOX2) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P48431
Target Symbol	SOX2
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM
Expression Range	1-317aa
Protein Length	Full Length
Mol. Weight	38.4 kDa
Research Area	Transcription
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Binds to the proximal enhancer region of NANOG. Critical for early embryogenesis and for embryonic stem cell pluripotency. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. May function as a switch in neuronal development.
Subcellular Location	Nucleus.
Database References	HGNC: 11195 OMIM: 184429 KEGG: hsa:6657 STRING: 9606.ENSP00000323588 UniGene: PMID: 30190462 Results demonstrated that SOX2 is a target of miR200c in MCF7/Tax cells. Knockdown of SOX2 expression increased chemosensitivity to paclitaxel and upregulated miR200c expression in MCF7/Tax cells. PMID: 30272330 BCL11A is integral to lung squamous cell carcinoma pathology and highlights the disruption of the BCL11A-SOX2 transcriptional programme as a novel candidate for drug development. PMID: 30127402 Hedgehog signaling (HH) and SOX2 function are inclined to developmental cues and arbitrating malignant growth in prostate cancer. SOX2 inhibition reduces cell proliferation and induces apoptosis. Conjoint inhibition of SOX2 and HH pathway induce apoptosis, reduce proliferation and migration. PMID: 27816521 The results remind us that clinical anophthalmia may be accompanied by sensorineural hearing loss and may be associated with SOX2 mutation, and it will contribute to improving diagnosis and patient care. PMID: 30262714 Metastatic double primary endometrioid endometrial and ovarian carcinoma sections expressed a higher level of SOX2 compared to the corresponding DPEEOC tissues. PMID: 29569698 The invasion and migration of osteosarcoma cells were down-regulated significantly through the inhibition of Sox2 by inactivating Wnt/beta-catenin signaling pathway related proteins PMID: 29619662 SOX2 is a critical oncogene linked to cancer stemness properties in urothelial carcinoma of the bladder. PMID: 29180467 Six SOX2 positive synovial sarcoma cases were analyzed by FISH using a SOX2/CEN3 dual color FISH probe PMID: 29773426 Tumor tissue Sox2 expression was not positively correlated with histologic grade, pathological tumor size and prognosis in surgical patients with triple-negative breast cancer. PMID: 29536377 Study in glioma stem-like cells (GSCs) identified SOX2 as one of downstream METTL3 targets and further recruitment of HuR onto m6A-modified sites in SOX2 is essential for SOX2 mRNA stabilization. METTL3 alters the DNA repair efficiency and radiation sensitivity partially via SOX2 in GSCs. PMID: 28991227 Myeloid Zinc Finger 1 and GA Binding Protein Co-Operate with Sox2 in Regulating the Expression of Yes-Associated Protein 1 in Cancer Cells PMID: 28905448 Human cytomegalovirus IE1 causes SOX2 downregulation by promoting the nuclear accumulation and inhibiting the phosphorylation of STAT3, a transcriptional activator of SOX2 expression. PMID: 29950413 hypopharyngeal squamous cell carcinomas characteristically almost completely lacking SOX2 expression appeared to be aggressive neoplasms with high recurrence rates. Promoter hypermethylation was determined to be a major mechanism underlying epigenetic SOX2 silencing PMID: 29143365 this study shows that basaloid squamous cell carcinoma and basal cell carcinoma of the head and neck can be readily distinguished by a limited panel consisting primarily of EMA, and supported by SOX2 and p16 PMID: 27438511 Results show that the levels of small nucleolar RNA host gene 5 (SNHG5) and SRY (sex determining region Y)-box 2 protein (SOX2) were significantly downregulated in osteoarthritis (OA) tissues, while the level of miR-26a was upregulated. PMID: 29409014 SOX2 copy number increases are detectable in a substantial proportion of high-grade HPV-positive vulvar carcinomas with basaloid differentiation. PMID: 28700423 The staining patterns did not reliably distinguish cervical intraepithelial neoplasia, grade 3 (CIN 3) from CIN 3-like Squamous cell carcinoma (SCC). Small infiltrative tumor nests around the margins of CIN 3 or deeply invasive CIN 3-like SCC often showed a localized reduction in SOX2 expression suggesting SOX2 downregulation during the transition to invasive growth. PMID: 28319578 Low SOX2 expression marks a distinct subset of adenoid cystic carcinoma of the head and neck and is associated with an advanced tumor stage PMID: 29596469 SOX2 overexpression in DPSCs promoted the regulation of odontoblast differentiation of DPSCs PMID: 29039570 SOX2 expression is dependent on contact with enhancers 700 kb downstream via CTCFdependent chromatin looping. PMID: 29091765 LncRNA SOX2-OT is a novel prognostic biomarker for osteosarcoma patients, it regulates osteosarcoma cells proliferation and motility through SOX2 modulation, and is correlated with patients' survival. PMID: 28960757 SOX2 overexpression is associated with cancer. PMID: 29587142 Histone acetyltransferase EP300 is necessary for the transcription factor SOX2 activity in basal cells, including for induction of the squamous fate. EP300 copy number gains are common in squamous cell carcinoma SQCCs, including lung cancer SQCC cell lines. PMID: 28794006 NFATc2 enhances tumor-initiating phenotypes through the NFATc2/SOX2/ ALDH1A1 axis in lung adenocarcinoma. PMID: 28737489 Block the activation of the VRK1 promoter by Sox2. PMID: 27334688 Heterozygous loss-of-function mutations in SOX2 are the commonest cause of severe microphthalmia and anophthalmia. PMID: 28635421 This data demonstrated immunogenicity of SOX2, which is specifically overexpressed on pediatric glial tumor cells. PMID: 28620836 OCT4 and SOX2 work as transcriptional activators in reprogramming human fibroblasts. PMID: 28813671 Sox2-expressing cells mark a subpopulation of tumor cells that fuel the growth of established BCa. SOX2-positive cells also expressed other previously reported BCa CSC markers, including Keratin14 (KRT14) and CD44v6. Ablation of Sox2-expressing cells within primary invasive BCa led to enhanced tumor regression PMID: 28793245 studies on the role of SOX2 in breast cancer may provide effective biomarkers and potential therapeutic targets for the diagnosis and treatment of breast cancer PMID: 28674712 In conclusion, targeted sequencing for SOX2 and VSX2 identified the etiology in two patients (7.4%) and this is the first report of SOX2 mutation from Egypt. PMID: 28121235 Kat2b are essential to the differentiation through enhancing recruitment neuroectodermal factors Sox2 and Pax6 to their target sites. PMID: 28475175 combined administration of small-interfering RNA (siRNA) transfection with PPARgamma ligands induced downregulation of SOX2 and MMP2 activity together with inhibition of sphere-forming activity regardless of poly(ADP-ribose) polymerase (PARP) cleavage. Taken together, our findings suggest that a combination therapy using PPARgamma ligands and its inhibitor could be a potential therapeutic strategy targeting glioblastom... PMID: 28642874 DDX53 directly regulates the SOX-2 expression in drug-resistant melanoma cells. PMID: 28535666 Rectal tumor tissue OCT4 (p<0.001), SOX2 (p=0.003), and NANOG (p<0.001) expressions were higher than those in adjacent tissue. PMID: 29214774 Thus, SOX2 is a master regulator of the acinar cell lineage essential to the establishment of a functional organ. PMID: 28623666 Results provide evidence that SOX2 expression is regulated by the transcription factor HSF1. PMID: 29316077 SOX2 overexpression and the loss of Rb1 protein expression might have a pivotal role in the divergent differentiation of pluripotent embryonic-like epithelial cells and the development of esophageal small-cell carcinoma. PMID: 28106103 In SOX2-deficient cells, BMP signaling is inhibited, but NODAL signaling is not activated. Thus, SOX2 appears to be downstream of BMP signaling but upstream of NODAL activation. PMID: 27283990 Results show lower SOX2 in the lymph node metastases from head and neck squamous cell carcinomas (HNSCCs) and in cervical cancer of unknown primary patients compared with the primary tumor. Tumors from N1 to N3 stage exhibit decreasing SOX2 expression in the lymphatic metastases. Its expression is showed to be involved in the proliferation and migration of HNSCC cell lines. PMID: 28002801 SOX2 overexpression could be used as a positive prognostic factor in patients with stage III lung squamous cell carcinoma receiving adjuvant radiotherapy PMID: 26323639 The survival rate was increased by 11.1% in Oct4/Sox2-hAT-MSC-injected mice. PMID: 28438862 hypoxia-NOTCH1-SOX2 signaling axis is important for activation of ovarian cancer stem cells. PMID: 27489349 This severe movement disorder appears to be a feature of some types of SOX2-anophthalmia syndrome thus expanding the phenotype. We strongly recommend that all newborns with bilateral anophthalmia/microphthalmia be screened for mutations in SOX2 PMID: 27427475 Findings indicate that long-term exposure to IM results in dysregulation of stem cell renewal-regulatory Hippo (MST1/2)/YAP signaling, and that inhibition of miR-181a using a microRNA sponge inhibitor resulted in decreased transcription of SOX2 and SALL4. PMID: 28103766 The authors found that high/positive SOX2 alterations, either DNA amplification or protein expression, were favorable for overall survival (OS) in non-small cell lung cancer. On the contrary, high/positive Nestin protein expression was poor for OS. PMID: 27150062 Data show that tunicamycin reduces the expression of self-renewal regulator Sox2 at translation level. PMID: 27119230 Increasing SOX2 reduces growth inhibition mediated by MEK and AKT inhibitors; whereas knockdown of SOX2 further reduces growth when Pancreatic ductal adenocarcinoma cells are treated with these inhibitors. Thus, targeting SOX2, or its mode of action, could improve the treatment of Pancreatic ductal adenocarcinoma. PMID: 27145457 This study discloses novel SOX2 target genes driving neuroendocrine differentiation and spread of PC and proposes SOX2 as a functional biomarker of LN metastasization for prostate cancer. PMID: 26540632

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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