Recombinant Human Transcription Factor 7-Like 2 (TCF7L2) Protein (His)

Name: Recombinant Human Transcription Factor 7-Like 2 (TCF7L2) Protein (His)
Brand: Beta LifeScience
SKU: BLC-08662P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Transcription Factor 7-Like 2 (TCF7L2) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q9NQB0
Target Symbol	TCF7L2
Synonyms	HMG box transcription factor 4; hTCF 4; hTCF-4; T cell factor 4; T cell specific HMG box; T cell specific transcription factor 4; T-cell factor 4; T-cell-specific transcription factor 4; TCF 4; TCF-4; TCF4; TCF7L2; TCF7L2 protein; TF7L2_HUMAN; Transcription factor 7 like 2; Transcription factor 7 like 2 T cell specific HMG box; Transcription factor 7-like 2
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPPRSESFRDKSRESLEEAAKRQDGGLFKGPPYPGYPFIMIPDLTSPYLPNGSLSPTARTLHFQSGSTHYSAYKTIEHQIAVQYLQMKWPLLDVQAGSLQSRQALKDARSPSPAHIVSNKVPVVQHPHHVHPLTPLITYSNEHFTPGNPPPHLPADVDPKTGIPRPPHPPDISPYYPLSPGTVGQIPHPLGWLVPQQGQPVYPITTGGFRHPYPTALTVNASMSRFPPHMVPPHHTLHTTGIPHPAIVTPTVKQESSQSDVGSLHSSKHQDSKKEEEKKKPHIKKPLNAFMLYMKEMRAKVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKRDKQPGETNEHSECFLNPCLSLPPIT
Expression Range	1-456aa
Protein Length	Partial
Mol. Weight	54.9kDa
Research Area	Epigenetics And Nuclear Signaling
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Participates in the Wnt signaling pathway and modulates MYC expression by binding to its promoter in a sequence-specific manner. Acts as repressor in the absence of CTNNB1, and as activator in its presence. Activates transcription from promoters with several copies of the Tcf motif 5'-CCTTTGATC-3' in the presence of CTNNB1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest in G1. Necessary for the maintenance of the epithelial stem-cell compartment of the small intestine.
Subcellular Location	Nucleus, PML body. Note=Diffuse pattern. Colocalizes with SUMO1 and PIAS4 in a subset of PML (promyelocytic leukemia) nuclear bodies.
Protein Families	TCF/LEF family
Database References	HGNC: 11641 OMIM: 125853 KEGG: hsa:6934 STRING: 9606.ENSP00000444972 UniGene: PMID: 28263491 The meta-analysis suggested that TCF7L2 polymorphism rs7903146 was significantly associated with type 2 diabetes mellitus in Caucasian, East Asian, South Asian and other ethnicities. [Meta-analysis] PMID: 29514658 The responses of insulin and HOMA-IR to ALE supplementation have shown an interaction with single-nucleotide polymorphism rs7903146 in TCF7L2. PMID: 30177026 The two varients of the gene TCF7L2 are important genetic risk factors for the T2D development in the Han ethnic group in China. PMID: 30266127 In conclusion, homozygous TT allele carriers show altered postprandial triglyceride response, mainly influencing very low density lipoproteins and high density lipoproteins subclasses suggesting a genotype-mediated effect on hepatic lipid regulation. PMID: 28220878 a considerable connection of DEFB1 and TCF7L2 gene polymorphisms with nephrolithiasis PMID: 29959006 stable knockdown of FOXO3, NCOA3, and TCF7L2 restored growth in low glucose but reduced MEK/MAPK phosphorylation, reduced anchorage-independent growth, and modulated expressions of GLUT1 and Ras pathway related proteins. PMID: 29301589 Study showed the association of TCF7L2 SNPs (rs7903146, rs12255372, and rs10885406) with high total cholesterol/high-density lipoproteins ratio. Findings highlight the influence of TCF7L2 SNPs on altered lipid profile in the Balinese, which may further link to the risk of cardiovascular diseases. PMID: 30027476 TCF7L2 rs7903146 polymorphism may be associated with the susceptibility to diabetic nephropathy in Chinese Han population, but rs290487 is not. The strong linkage disequilibrium existed between the 2 single nucleotide polymorphisms and haplotype T-T (rs7903146-rs290487) increased the susceptibility to diabetic nephropathy. PMID: 30290587 the T risk allele of the rs7903146 in the TCF7L2 gene increases the risk of type 2 diabetes, was determined. PMID: 29631902 Common variation at TCF7L2 influences acute responses to both glipizide and metformin in people without diabetes and highlight altered incretin signaling as a potential mechanism by which TCF7L2 variation increases T2D risk. PMID: 29326107 No correlation between the studied polymorphisms of the TCF7L2 gene and GDM was observed. PMID: 27958632 Type 1 diabetes mellitus patients carriers of the TCF7L2 variant had a milder immunologic and metabolic phenotype at type 1 diabetes diagnosis, which could be partly driven by type 2 diabetes-like pathogenic mechanisms. PMID: 29025879 The SNPs in TCF7L2 and HHEX were genotyped by polymerase chain reaction-restriction fragment length polymorphism. There were no significant differences in the distribution of genotypes and alleles between polycystic ovary syndrome cases and controls PMID: 26563606 rs122555372 was associated with pancreatic reserve in patients with type 2 diabetes and with fasting glucose and beta-cell function in individuals without diabetes PMID: 28101933 TCF7L2 mRNA expression is downregulated in humans with impaired glucose tolerance and adipocyte insulin resistance. PMID: 29317436 PGC-1alpha induction during differentiation is required for both mitochondrial biogenesis and commitment to the hepatocytic lineage, and TCF7L2 repression is sufficient to increase PGC-1alpha expression, mitochondrial biogenesis and OXPHOS activity. PMID: 28795454 role in dermal papilla cell proliferation PMID: 24354472 Silencing the tcf4 gene confers sensitivity to oxaliplatin in colorectal cancer cells. PMID: 24869759 GRbeta bound to the N-terminus domain of TCF-4 its influence on Wnt signaling required both ligand- and DNA-binding domains. This is enough to maintain the TCF/LEF activity at a high level in the absence of beta-catenin stabilization. PMID: 25301232 TRIB2 negatively regulates Wnt activity through a reduction in protein stability of TCF4 and beta-Catenin PMID: 25311538 CtBP physically interacted with TCF-4, and this interaction was significantly inhibited in the presence of MTOB. The above findings point to a novel role of CtBPs in the promotion of CSC growth and self-renewal PMID: 25483087 Our data revealed that low TCF4 protein expression was a useful predictive factor of good tumor response to nCRT and good outcomes in patients with LARC. PMID: 25519018 These results indicate that a dynamic interplay of TCF transcription factors governs MYC gene expression in colorectal cancers. PMID: 25659031 KLF5 facilitates lysophosphatidic acid -induced interaction between beta-catenin and TCF4. PMID: 25683913 The E3 ligase RNF43 inhibits Wnt signaling downstream of mutated beta-catenin by sequestering TCF4 to the nuclear membrane. PMID: 26350900 Results provides evidence that TCF4 and ZEB1 modulate each other's transcriptional activity in the regulation of tumor pro-invasive Wnt target genes. PMID: 26387539 The expression of NLK was negatively correlated with TCF4 expression in lung cancers PMID: 26823848 the beta-catenin/Tcf4 interaction is disrupted by BC-23 PMID: 27014877 Data show that HMG-box transcription factor 1 protein HBP1-mediated elevation of CDK inhibitor p21 through the Mdm2/p53 and TCF4/EZH2 pathways contributes to both cellular senescence and tumor inhibition. PMID: 27129219 These results may provide a linkage between PCa chemoresistance and exosome regulatory networks and thus lead us to propose that AR, PTEN and TCF4 genes may be the important genes which are regulated by exosome miRNAs in chemoresistance cancer cells. PMID: 27278879 G-17 caused the overexpression of beta-catenin and TCF-4. PMID: 27430592 Taken together, these results suggest that this newly identified Rock2-beta-catenin/TCF4-SCARA5 axis will provide novel insight into the understanding of the regulatory mechanisms of proliferation in human RCC. PMID: 27793664 we observed Dickkopf-related protein 3 (DKK3) as a direct target of miR-25 in vitro. Upregulation of DKK3 partially attenuated the oncogenic effect of miR-25 on melanoma cells. Ectopic expression of miR-25 in melanoma cells induced beta-catenin accumulation in nuclear and inhibited TCF4 (T cell factor 4) activity, as well as the expression of c-Myc and Cyclin D1. PMID: 27801786 FOXN3 bind to beta-catenin and inhibited beta-catenin/TCF signaling by blocking the interaction between beta-catenin and TCF4. Loss of FOXN3 in colon cancer activates beta-catenin/TCF signaling and promotes the growth and migration of cancer cells. PMID: 28039460 High TCF4 expression is associated with colorectal cancer. PMID: 28921929 Overexpression of vPK led to reduced mRNA expression of cyclin D1, a well-known transcriptional product of Wnt signaling, suggesting that vPK effectively regulates the host signaling pathway through direct interactions with cellular proteins. PMID: 29432739 TCF7L2 rs290487, rs6585194, and rs7094463 polymorphisms were found to be significantly associated with gestational diabetes mellitus. PMID: 27465520 TCF7L2 rs7903146 polymorphism is associated with ischemic heart disease. PMID: 28299838 The IVS3C>T locus in the TCF7L2 gene is not independently statistically significantly associated with the development of type 2 Diabetes Mellitus in the Kyrgyz population. PMID: 29171469 six of eight SNPs were found to have significant associations between TCF7L2 variants and gestational diabetes mellitus (GDM) risk in the overall population, with the most powerful SNPs being rs7903146, rs12255372 and rs7901695, but the contribution of these SNPs to GDM risk were variable among different racial/ethnic groups - meta-analysis PMID: 28002648 These results suggest rs12573128 is significantly associated with an increased risk of SCZ in the Chinese Han population. PMID: 28404897 The study found that TCF7L2 SNPs (rs1225404 and rs7003146) might be associated with breast cancer risk in Northwest Chinese Han populations. PMID: 27738320 These findings further support the hypothesis that TCF7L2 gene variation contributes to diabetogenesis in a subset of young people with Type 1 diabetes. PMID: 27027642 The rs7903146 variant in the TCF7L2 gene increases the risk of impaired glucose tolerance or type 2 diabetes in obese adolescents by impairing beta-cell function, and hepatic insulin sensitivity predicts the development of impaired glucose tolerance or type 2 diabetes over time. PMID: 28611053 In conclusion, TCF7L2 regulates estradiol- or progesterone-modulated islet and hepatic glucose metabolism. PMID: 27108846 TCF7L2 rs7903146 and 112/112 haplotype of CAPN10 might be associated with gestational diabetes risks.[meta-analysis] PMID: 28277135 Authors found that KIF23 was regulated by TCF-4 at transcriptionally level. Therefore, this evidence indicates KIF23 over-expression is associated with glioma malignancy and conferred a worse survival time in glioma. PMID: 27013586 findings indicate that the association between TCF7L2 SNP rs12255372 and HDL-C may be modified by dietary fat intake in this Asian Indian population PMID: 29182660 Wnt Signaling Pathway Effector TCF7L2 plays a role in Glucose Homeostasis. PMID: 27159876

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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