Recombinant Human Toll-Like Receptor 8 (TLR8) Protein (His)

Name: Recombinant Human Toll-Like Receptor 8 (TLR8) Protein (His)
Brand: Beta LifeScience
SKU: BLC-04548P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Toll-Like Receptor 8 (TLR8) Protein (His) is produced by our Yeast expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q9NR97
Target Symbol	TLR8
Synonyms	CD 288; CD288; CD288 antigen; MGC119599; MGC119600; TLR 8; Tlr8; TLR8_HUMAN; Toll like receptor 8; Toll-like receptor 8
Species	Homo sapiens (Human)
Expression System	Yeast
Tag	N-6His
Target Protein Sequence	EENFSRSYPCDEKKQNDSVIAECSNRRLQEVPQTVGKYVTELDLSDNFITHITNESFQGLQNLTKINLNHNPNVQHQNGNPGIQSNGLNITDGAFLNLKNLRELLLEDNQLPQIPSGLPESLTELSLIQNNIYNITKEGISRLINLKNLYLAWNCYFNKVCEKTNIEDGVFETLTNLELLSLSFNSLSHVPPKLPSSLRKLFLSNTQIKYISEEDFKGLINLTLLDLSGNCPRCFNAPFPCVPCDGGASINIDRFAFQNLTQLRYLNLSSTSLRKINAAWFKNMPHLKVLDLEFNYLVGEIASGAFLTMLPRLEILDLSFNYIKGSYPQHINISRNFSKLLSLRALHLRGYVFQELREDDFQPLMQLPNLSTINLGINFIKQIDFKLFQNFSNLEIIYLSENRISPLVKDTRQSYANSSSFQRHIRKRRSTDFEFDPHSNFYHFTRPLIKPQCAAYGKALDLSLNSIFFIGPNQFENLPDIACLNLSANSNAQVLSGTEFSAIPHVKYLDLTNNRLDFDNASALTELSDLEVLDLSYNSHYFRIAGVTHHLEFIQNFTNLKVLNLSHNNIYTLTDKYNLESKSLVELVFSGNRLDILWNDDDNRYISIFKGLKNLTRLDLSLNRLKHIPNEAFLNLPASLTELHINDNMLKFFNWTLLQQFPRLELLDLRGNKLLFLTDSLSDFTSSLRTLLLSHNRISHLPSGFLSEVSSLKHLDLSSNLLKTINKSALETKTTTKLSMLELHGNPFECTCDIGDFRRWMDEHLNVKIPRLVDVICASPGDQRGKSIVSLELTTCVSDVT
Expression Range	27-827
Protein Length	partial
Mol. Weight	93.5kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Endosomal receptor that plays a key role in innate and adaptive immunity. Controls host immune response against pathogens through recognition of RNA degradation products specific to microorganisms that are initially processed by RNASET2. Recognizes GU-rich single-stranded RNA (GU-rich RNA) derived from SARS-CoV-2, SARS-CoV-1 and HIV-1 viruses. Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction. In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce proinflammatory cytokines and interferons, respectively.
Subcellular Location	Endosome membrane; Single-pass type I membrane protein.
Protein Families	Toll-like receptor family
Database References	HGNC: 15632 OMIM: 300366 KEGG: hsa:51311 STRING: 9606.ENSP00000218032 UniGene: PMID: 27677832 MBZ-induced IL-1beta release was found to be dependent on NLRP3 inflammasome activation and to involve TLR8 stimulation. PMID: 28472897 the current study shows that motolimod ( selective small-molecule agonist of TLR8, stimulates natural killer (NK) cells, dendritic cells, and monocytes))can be safely administered in combination with cetuximab with an acceptable toxicity profile PMID: 27810904 Epstein-Barr virus (EBV) replication activating the toll like receptor 8 (TLR8) molecular pathway in primary monocytes. PMID: 28245863 this study identified TLR8 as mediator of monocyte differentiation and M2 macrophage polarization during hepatitis C virus infection PMID: 28122964 Demonstrate STAT1-dependent transcriptional activation of TLR8 with estrogen stimulation. PMID: 28039018 TLR8 gene rs3764880 polymorphisms might be associated with susceptibility to ischemic stroke and involved in the inflammatory reaction and lipid metabolism of ischemic stroke in southern Chinese Han. PMID: 27772795 this study shows that TLR8 gene polymorphism is associated with susceptibility to HIV and HCV co-infection PMID: 28062211 Comparison of present and previous association studies reveals contradictory results for common variants. Thus, no associations exist between genetic variation in TLR8 and AR. PMID: 26556310 Identified human TLR8 as functional TLR13 equivalent that promiscuously senses ssRNA. PMID: 26545385 TLR8 polymorphisms seem to be related to non-progression of liver fibrosis in HIV/HCV coinfected patients, particularly in males and those patients infected with GT1. PMID: 26455634 TLR8 with the uncleaved Z-loop is unable to form a dimer. The uncleaved Z-loop located on the ascending lateral face prevents the approach of the dimerization partner by steric hindrance. PMID: 26929371 Sex-specific associations for TLR8 polymorphisms in tuberculosis. PMID: 26160538 Knockdown of LRRC59 reduced TLR3-, 8-, and 9-mediated, but not TLR4-mediated, signaling. PMID: 26466955 the activation of TLR8 by bi-specific si-RNA for the production of INFbeta, was investigated. PMID: 26202481 Results suggest a relationship between the TLR8 and the susceptibility to Crohn's disease and Behcet's disease. PMID: 26486764 Carriage of the G-allele of TLR8-rs3764879 was associated with the occurrence of SLE in Danish women, but not men. PMID: 24919757 TLR8 is identified as receptor for bacterial RNA in primary human monocyte-derived macrophages. Moreover, TLR8-dependent detection of bacterial RNA was critical for triggering monocyte activation in response to infection with Streptococcus pyogenes. PMID: 26101323 This study demonstrates a physiological role of TLR8 in the sensing of entire S. aureus in human primary phagocytes, including the induction of IFN-beta and IL-12 production via a TAK1-IKKbeta-IRF5 pathway that can be inhibited by TLR2 signaling. PMID: 26085680 No significant associations have been found between TLR8 polymorphisms and susceptibility to Graves' disease in Chinese Cantonese. PMID: 25430040 Met1Val of TLR8 is significantly associated with risk of pulmonary tuberculosis susceptibility in southern Punjab population. This effect was highly pronounced among males. PMID: 25572425 The minor allele of TLR8 rs3764879 of the donor is associated with outcome after myeloablative conditioned allogeneic hematopoietic cell transplantation. PMID: 25464115 TLR8, but not TLR7, is involved in priming of human neutrophil reactive oxygen species production by inducing the phosphorylation of p47phox and p38 MAPK. PMID: 25877926 Increased miR-K-10b and miR-K12-12 are functionally involved in sepsis as agonists of TLR8, forming a positive feedback that may lead to cytokine dysregulation. PMID: 25476907 Data show that activation of toll-like receptor 8 (TLR8) signaling in tumor cells can block the induction and reverse the suppression of senescent naive and tumor-specific T cells in vitro and in vivo, resulting in enhanced anti-tumor immunity. PMID: 25231413 Data indicate that treatment with toll-like receptor 8 (TLR8) agonists elicited granzyme B production. PMID: 25667415 TLR8 activation has direct anti-leukemic effects independent of its immunomodulating properties. PMID: 25283842 The insertion loop between leucine-rich repeats 14 and 15 in TLR8 is indispensable for the receptor cleavage in endosomes. PMID: 25297876 HIV-1 infection induced the expression of pro-IL-1beta via TLR8-mediated mechanisms and activated caspase-1 through the NLRP3 inflammasome to cleave pro-IL-1beta into bioactive IL-1beta PMID: 24939850 The mRNA expression level of TLR8 in the patients with cervical cancer and Hela cells were up-regulated, it consistent with the increased expression of VEGF and Bcl-2. PMID: 24782650 Data indicate 4-methyl-1-pentyl-1H-benzo[d]imidazol-2-amine a pure toll-like receptor 8 (TLR8) agonist, evoking proinflammatory cytokine and Type II interferon responses in mononuclear leukocytes. PMID: 25102141 non-synonymous TLR8 SNP rs3764880-G allele was a risk factor for oral ulcer and pericardial effusion, with significant additive effects PMID: 24445780 Variations in TLR8 genes may modulate immune responses during HCV infection. PMID: 24205871 TLR8 is a novel estrogen target gene that can lower the inflammatory threshold and implicate an IFNalpha-independent inflammatory mechanism that could contribute to higher systemic lupus erythematosus incidence in women. PMID: 24525049 The mechanisms of action of Aldaratrade mark are complex. Imiquimod is an effective ligand for TLR7 (and TLR8 in humans) and also interferes with adenosine receptor signaling. PMID: 24254490 Bb RNA is a TLR8 ligand in human monocytes and that transcription of IFN-beta in response to the spirochete is induced from within the phagosomal vacuole through the TLR8-MyD88 pathway. PMID: 23906644 Data indicate that TLR8 stimulation of NK-DC co-cultures significantly increased DC priming of EGFR-specific CD8(+) T cells in the presence of cetuximab. PMID: 23685782 TLR8 expression and signaling is impaired in peripheral blood mononuclear cells from patients with multiple sclerosis PMID: 23787171 This study demonstrates profibrotic properties of circulating monocytes from patients with systemic sclerosis and a key role for TLR signalling, particularly TLR8, in TIMP-1 secretion and matrix remodelling. PMID: 23223421 crystal structures of unliganded and ligand-induced activated TLR8 dimers were elucidated; ligand binding induces reorganization of the TLR8 dimer, which enables downstream signaling processes PMID: 23520111 Single nucleotide polymorphism in the TLR8 gene is associated with allergic rhinitis. PMID: 22857391 IL10 production after TLR9 ligands or HSV-1 stimulation was significantly related with plasma levels of sex hormones, whereas no correlation was found in cytokines produced following TLR7 and TLR8 stimulation. PMID: 22768144 Expression of TLR8 is significantly increased in HeLa cells. TLR8 agonist may influence tumor development. PMID: 22340042 UNC93B1 physically associates with human TLR8 and regulates TLR8-mediated signaling PMID: 22164301 Epigenetic regulation of tumor necrosis factor alpha (TNFalpha) release in human macrophages by HIV-1 single-stranded RNA (ssRNA) is dependent on TLR8 signaling PMID: 22393042 analyzed the frequency of TLR7 rs179008, TLR8 rs3764880, TLR9 rs5743836 and rs352140 in 370 patients with SLE and 415 healthy controls from southern Brazil PMID: 22065095 Human TLR8 detectS viral single-stranded RNA and imidazoquinoline compounds. Review. PMID: 22289837 Quantitative polymerase chain reaction showed significantly higher expression of LEAP-1 (P = 0.002) and TLR-8 (P = 0.023) and TLR-10 (P = 0.014) in viral keratitis and LEAP-2 (P = 0.034) in dry eye, versus controls. PMID: 21499082 Lower toll-like receptor 8 expression was observed in monocytes from infants with respiratory syncytial virus infection. PMID: 20946625 Phagosomal signaling involves a cooperative interaction between TLR2 and TLR8 in pro- and antiinflammatory cytokine responses, whereas TLR8 is solely responsible for IRF7-mediated induction of IFN-beta. PMID: 21321205

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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Recombinant Human Toll-Like Receptor 8 (TLR8) Protein (His)

Recombinant Human Toll-Like Receptor 8 (TLR8) Protein (His)

Product Overview

Target Details

FAQs