Recombinant Human Tnfaip3-Interacting Protein 1 (TNIP1) Protein (His&Myc)

Name: Recombinant Human Tnfaip3-Interacting Protein 1 (TNIP1) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-06540P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Tnfaip3-Interacting Protein 1 (TNIP1) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q15025
Target Symbol	TNIP1
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	MEGRGPYRIYDPGGSVPSGEASAAFERLVKENSRLKEKMQGIKMLGELLEESQMEATRLRQKAEELVKDNELLPPPSPSLGSFDPLAELTGKDSNVTASPTAPACPSDKPAPVQKPPSSGTSSEFEVVTPEEQNSPESSSHANAMALGPLPREDGNLMLHLQRLETTLSVCAEEPDHGQLFTHLGRMALEFNRLASKVHKNEQRTSILQTLCEQLRKENEALKAKLDKGLEQRDQAAERLREENLELKKLLMSNGNKEGASGRPGSPKMEGTGKKAVAGQQQASVTAGKVPEVVALGAAEKKVKMLEQQRSELLEVNKQWDQHFRSMKQQYEQKITELRQKLADLQKQVTDLEAEREQKQRDFDRKLLLAKSKIEMEETDKEQLTAEAKELRQKVKYLQDQLSPLTRQREYQEKEIQRLNKALEEALSIQTPPSSPPTAFGSPEGAGALLRKQELVTQNELLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQAQVTLSNAQLKAFKDEEKAREALRQQKRKAKASGERYHVEPHPEHLCGAYPYAYPPMPAMVPHHGFEDWSQIRYPPPPMAMEHPPPLPNSRLFHLPEYTWRLPCGGVRNPNQSSQVMDPPTARPTEPESPKNDREGPQ
Expression Range	1-636aa
Protein Length	Full Length
Mol. Weight	76.9 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Inhibits NF-kappa-B activation and TNF-induced NF-kappa-B-dependent gene expression by regulating A20/TNFAIP3-mediated deubiquitination of IKBKG; proposed to link A20/TNFAIP3 to ubiquitinated IKBKG. Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway; blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcription. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity; this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflammation; this function is mediated by association with SELPLG and dependent on phosphorylation by SRC-family kinases. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. In case of infection, promotes association of IKBKG with Shigella flexneri E3 ubiquitin-protein ligase ipah9.8 p which in turn promotes polyubiquitination of IKBKG leading to its proteasome-dependent degradation and thus is perturbing NF-kappa-B activation during bacterial infection.
Subcellular Location	Cytoplasm. Nucleus. Note=Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner.
Database References	HGNC: 16903 OMIM: 607714 KEGG: hsa:10318 STRING: 9606.ENSP00000317891 UniGene: PMID: 30402506 Results showed that ABIN1 acted as a modulator to down-regulate HDAC1 ubiquitination via three different sites, thereby stabilizing HDAC1 by inhibiting its lysosomal and proteasomal degradation. PMID: 29058807 Results provide evidence that TNIP1 SNP (rs7708392) might be associated with the development of lupus nephritis in Egyptian SLE patients. PMID: 29589214 cell viability and apoptosis in the hepatic cell line L02 were detected after hypoxia/reoxygenation (H/R) treatment, and MTT assay showed that cell viability was inhibited after H/R treatment, but reversed after TNIP1 transfection PMID: 29709475 association of TNIP1 was stronger in AIH without HLA-DRB104:05 allele (P = 0.0063, Q = 0.0127, OR 1.48, 95% CI: 1.12-1.96), though the association was not detected in AIH with DRB104:05 PMID: 29559739 Naf1 contributes to the maintenance of HIV-1 latency by inhibiting long terminal repeat driven HIV-1 gene transcription in a nuclear factor kappa B-dependent manner. PMID: 27795436 the risk allele for the TNIP1 rs7708392 polymorphism was associated with systemic lupus erythematosus susceptibility. PMID: 29783072 C/EBP beta LAP isoform expression was increased and LIP/TNFAIP3/TNIP1 expression was decreased in systemic lupus erythematosus (SLE) patients. LAP expression was positively correlated with SLE disease activity; TNFAIP3 and TNIP1 expression was negatively correlated with SLE disease activity. PMID: 27659348 genetic variation in TNIP1 gene is associated with gastric carcinoma PMID: 27250029 An association of the GPX3-TNIP1 locus with ALS is identified using cross-ethnic meta-analyses. [Meta-Analysis] PMID: 28931804 These studies define a novel role for ABIN1 dysfunction and NF-kappaB in mediating glomerulonephritis through proinflammatory activation of podocytes. PMID: 28935578 IM could upregulate A20 protein to inhibit the activation of NF-kappaB pathway in Jurkat T cells, which was independent of the ABIN1 protein. PMID: 28502291 LILRB1 ligation during monocyte differentiation to dendritic cells in vitro increases ABIN1 expression. Increased ABIN1 expression occurs in "immunosuppressive" monocytes of patients with non-Hodgkin lymphoma ex vivo. ABIN1 siRNA allows dendritic cells and immunosuppressive monocytes to respond to stimulation by allowing NF-kappaB translocation to the nucleus, more phagocytosis, cytokine secretion, and T-cell stimulation. PMID: 27129285 These findings show ABIN1 as an intrinsic suppressor of HIV-1 mRNA transcription by regulating the ubiquitination of Tat. PMID: 28193275 Polymorphisms in TNIP1 are associated with GPP in Chinese Han population. However, no association with PPP was found. These findings suggest that TNIP1 might be a susceptibility gene for GPP. PMID: 27364786 The findings of this study suggested that TNIP1 is a new potential predisposing gene to spina bifida (SB) and its pathway needs to be investigated in human NTD in order to confirm its role and to plan appropriate counseling to families PMID: 27125519 Data suggest that reduced TNFalpha-induced protein 3-interacting protein 1 (TNIP1) expression may have a role in the pathogenesis of myasthenia gravis (MG)for patients with thymoma. PMID: 27577206 HIV-1 depends on host-cell-encoded factors to complete its life cycle; data suggest that nucleus-located NAF1/TNIP1 promotes nuclear export of un-spliced HIV-1 gag mRNA; association between NAF1/TNIP1 and CRM1 is required for this function. PMID: 26733199 Hepatitis C virus antigen stimulation up-regulates A20/A20-binding inhibitor of NF-kappaB binding protein expression, which consequently contributes to inefficient M1 macrophage polarization. PMID: 26382585 Data suggest that tumour necrosis factor alpha-induced protein 3 (TNFAIP3) and TNFAIP3-interacting protein 1 (TNIP1) single nucleotide polymorphisms (SNPs) confer increased risk of psoriasis in ethnic South Indian Tamils. PMID: 26738398 Since TNIP1 may facilitate the function of TNFAIP3 in the negative feedback regulation of NF-kappaB activation [5], the corresponding transcript levels of TNIP1 in psoriasis vul- garis were investigated in this study. PMID: 25631139 The aim of this study was to investigate whether the HCP5, TNIP1, TNFAIP3, SPATA2 and COG6 genes were genetic risk factors for psoriasis in Chinese population. PMID: 25264125 Single-nucleotide polymorphisms rs7708392 and rs495881 in TNIP1 were significantly associated with Lupus Nephritis in individuals with European or African ancestry. PMID: 25034154 HSPA6 regulation by TNIP1 occurs in promoter regions lacking binding sites for known TNIP1-repressed transcription factors PMID: 25447897 Report role of TNIP1 genetic variants in confering risk of systemic lupus erythematosus in Chinese population. PMID: 24091983 TNIP1 polymorphism may be a risk factor for VKH syndrome in Han Chinese PMID: 24788730 Analysis of SNP in the TNIP1 gene and expression of this gene in peripheral blood lymphocytes indicated these SNP were associated with the occurrence of systemic lupus erythematosus in Han Chinese patients. PMID: 23858047 We conclude that polymorphisms in TNIP1 are associated with antibody-positive primary Sjogren's syndrome. PMID: 23944604 variants in the TNIP1 gene are associated with the risk for lupus nephritis and could be mechanistically involved in disease development via aberrant regulation of NF-kappaB and mitogen-activated protein kinase activity. PMID: 23970121 TNIP1/ANXA6 and CSMD1 variants interacting with cigarette smoking and alcohol intake affect risk of psoriasis. PMID: 23541940 Data indicate significant multiplicative interaction observed between NFKB1 rs28362491 and TNIP1 rs3792783 in systemic lupus erythematosus. PMID: 23911423 Analysis of the TNIP1 promoter reveals multiple transcription start sites in its GC-rich proximal regions along with two transcriptionally active specificity protein (SP) sites, responsive to both Sp1 and Sp3. PMID: 23464785 These data confirmed the influence of TNIP1 on an increased susceptibility to systemic sclerosis and reinforced this locus as a common autoimmunity risk factor. PMID: 22896740 This study demonistrated that (151) Pro-->Ala change in TNIP1 association to myasthenia gravis. PMID: 23055271 Our results confirm the association signals between systemic lupus erythematosus and TNIP1 variants in multiple populations and provide new insight into the mechanism by which TNIP1 variants may contribute to SLE pathogenesis. PMID: 22833143 Data indicate that TNFAIP3, ETS1 and TNIP1 are probably common susceptibility genes for systemic lupus erythematosus (SLE) in Chinese populations, and they may contribute to the pathogenesis of multiple SLE subphenotypes. PMID: 22087647 we integrate the current knowledge of TNIP1s functions with the diseases in which it has been associated to potentially elucidate the role this regulator has in promoting or alleviating these inflammatory diseases[review] PMID: 22542476 The TNIP1 distal promoter, approximately 4600 nucleotides upstream of a frequently utilized transcription start site region, contains PPAR-gamma and NF-kappaB sites that bind the respective transcription factors in vivo. PMID: 22001530 recombinant transgene facilitates the transcription of pro-inflammatory cytokines in fibroblast-like synoviocytes in rheumatoid arthritis PMID: 22093807 A markedly reduced expression of the TNIP1 gene and also its protein product were observed both in lesional skin tissue and in cultured dermal fibroblasts from SSc(Systemic sclerosis) patients. PMID: 21750679 The requirements for TNIP1-peroxisome proliferator activated receptor (PPAR) interaction are characteristic for coactivators; however, TNIP1 partially decreases PPAR activity. PMID: 21967852 The varying levels and distribution of TNIP1 in normal and disease state tissues could be expected to affect processes in which TNIP1 is involved PMID: 22147607 The researchers found a genotype association of SNP rs1808593 NOS3 polymorphisms and HIE. PMID: 21674837 ABIN1 requires its ubiquitin binding domain and cooperates with TAX1BP1 and A20 to restrict antiviral signaling. PMID: 21885437 The IL23A and TNIP1 genes showed convincing evidence for association SNPs mapping to previously reported psoriasis loci show evidence for association to PSA. PMID: 21623003 the interaction of ABIN1 with polyubiquitin is required to limit the activation of TLR-MyD88 pathways and prevent autoimmunity PMID: 21606507 A20, ABIN-1/2, and CARD11 mutations have prognostic value in gastrointestinal diffuse large B-cell lymphoma PMID: 21266526 Association of TNIP1 with systemic lupus erythematosus was confirmed in a Japanese population PMID: 20849588 Significant associations were found for the single nucleotide polymorphism rs10036748 of TNIP1 with photosensitivity and vasculitis in patients with systemic lupus erythematosus PMID: 20516000 A new ERK2 binding protein, Naf1, attenuates the EGF/ERK2 nuclear signaling. PMID: 12220502

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

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