Recombinant Human Tnf Receptor-Associated Factor 3 (TRAF3) Protein (His)

Name: Recombinant Human Tnf Receptor-Associated Factor 3 (TRAF3) Protein (His)
Brand: Beta LifeScience
SKU: BLC-04804P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Tnf Receptor-Associated Factor 3 (TRAF3) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q13114
Target Symbol	TRAF3
Synonyms	CAP 1; CAP-1; CAP1 ; CD40 associated protein 1 ; CD40 binding protein ; CD40 bp; CD40 receptor associated factor 1; CD40 receptor-associated factor 1; CD40-binding protein; CD40BP; CRAF 1; CRAF1; IIAE5; LAP 1; LAP1; LMP1 associated protein ; LMP1-associated protein 1; MIPT3; TNF receptor associated factor 3 ; TNF receptor-associated factor 3; TRAF 3; Traf3; TRAF3_HUMAN
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	MESSKKMDSPGALQTNPPLKLHTDRSAGTPVFVPEQGGYKEKFVKTVEDKYKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKDKVFKDNCCKREILALQIYCRNESRGCAEQLMLGHLLVHLKNDCHFEELPCVRPDCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIALQKHEDTDCPCVVVSCPHKCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIKDDTIFIKVIVDTSDLPDP
Expression Range	1-568aa
Protein Length	Full Length
Mol. Weight	68.6 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.
Subcellular Location	Cytoplasm. Endosome. Mitochondrion.
Protein Families	TNF receptor-associated factor family, A subfamily
Database References	HGNC: 12033 OMIM: 601896 KEGG: hsa:7187 STRING: 9606.ENSP00000376500 UniGene: PMID: 30018345 Association between the rs1883832 and rs3765459 CD40 gene polymorphism and susceptibility to cervical cancer in a subset of Malaysian population. PMID: 28181356 Study demonstrated that TRAF3, as a novel RIP2 binding partner, was downregulated in glioma tissues and functionally was a negative regulator involved in RIP2induced glioma cell growth. PMID: 29693188 Data show that TNF receptor-associated factor 3 (TRAF3) autophagy is driven by RAS and results in activation of transcription factor RelB (RELB). PMID: 29146913 MicroRNA-214 regulates immunity-related genes in bovine mammary epithelial cells by targeting NFATc3 and TRA PMID: 28627449 These results indicate that TRAF3 deficiency suffices to metabolically reprogram B cells, a finding that improves our understanding of the role of TRAF3 as a tumor suppressor, and suggests potential therapeutic strategies. PMID: 27752131 Viral proteins aim to subvert TRAF3 antiviral action. PMID: 27208423 Mechanistic studies showed that HACE1 exerts its inhibitory role on virus-induced signaling by disrupting the MAVS-TRAF3 complex. PMID: 27213432 An important B cell-specific role for TNFR-associated factor 3 is the inhibition of homeostatic survival, directly relevant to the common occurrence of TNFR-associated factor 3 mutations in human B cell malignancies. Review. PMID: 27154354 The current investigations identified a subset of HPV-positive HNSCCs with mutations in the genes TRAF3 (tumor necrosis factor receptor-associated factor 3) and CYLD (cylindromatosis lysine 63 deubiquitinase). Defects in TRAF3 and CYLD correlated with the activation of transcriptional factor nuclear factor kappaB, episomal HPV status of tumors, and improved patient survival. PMID: 28295222 NDR1 interacts with TRAF3 and interferes with the association of TRAF3 and IL-17R, resulting in increased formation of the activation complex IL-17R-Act1, which is required for the downstream signaling and production of pro-inflammatory factors PMID: 28219902 Data suggest that UBR5 down-regulates levels of TRAF3, a key component of Toll-like receptor signaling, via the miRNA pathway; p90RSK is an upstream regulator of UBR5; p90RSK phosphorylates UBR5 as required for translational repression of TRAF3 mRNA. (UBR5 = ubiquitin protein ligase E3 component n-recognin 5 protein; TRAF3 = TNF receptor-associated factor 3; p90RSK = 90 kDa ribosomal protein S6 kinase) PMID: 28559278 The GA genotype and GA+AA genotype of TRAF3 rs12147254 were found to increase the risk of coronary heart disease among T2DM patients. the GACGAC haplotype in TRAF3 had a protective effect on T2DM micro-macrovascular complications. PMID: 28479387 These data suggest an interplay between CELF2 and hnRNP C as the mechanistic basis for activation-dependent alternative splicing of TRAF3 exon 8. PMID: 28031331 DDX3 directly regulates TRAF3 ubiquitination and acts as a scaffold to co-ordinate assembly of signaling complexes downstream from MAVS. PMID: 27980081 The NleB effector limited host IFN-beta production by inhibiting Lys(63)-linked ubiquitination of TNF receptor-associated factor 3 (TRAF3). Inhibition was dependent on the glycosyltransferase activity of NleB. PMID: 27387501 TRAF3 epigenetics may significantly contribute to the variability of clopidogrel response and recurrence of ischemic events in patients with stroke. PMID: 27026631 Taken all together, Helicobacter pylori toxin Tip-alpha might activate NF-kappaB to promote inflammation and carcinogenesis by inhibiting miR-3178 expression, which directly targets TRAF3, during Helicobacter pylori infection in gastric mucosal epithelial cells. PMID: 27493095 PLK1 overexpression in prostate epithelial cells triggers oncogenic transformation. The signaling mechanisms underlying the observed cellular effects of PLK1 involve direct PLK1-dependent phosphorylation of CRAF with subsequent stimulation of the MEK1/2-ERK1/2-Fra1-ZEB1/2 signaling pathway. PMID: 27003818 These findings suggest that RNF166 positively regulates RNA virus-triggered IFN-beta production by enhancing the ubiquitination of TRAF3 and TRAF6. PMID: 26456228 Findings establish CK1varepsilon as a regulator of antiviral innate immune responses and indicate a novel mechanism of immunoregulation that involves CK1varepsilon-mediated phosphorylation of TRAF3. PMID: 26928339 findings identify TRAF3 and PTPN22 as inhibitors of IL-6R signaling in B cells and reveal a previously uncharacterized role for TRAF3 in the regulation of plasma cell differentiation PMID: 26329582 TRAF3 regulates B-cell survival via inhibition of CREB stability PMID: 26755589 ESR1 directly interacts with TRAF3 and promotes K48-linked proteasomal degradation of TRAF3. PMID: 26186972 these findings demonstrate that WDR82 is a negative regulator of virus-triggered type I IFNs pathway through mediating TRAF3 polyubiquitination status and stability on mitochondria. PMID: 26519536 RIG-I-like receptor mediated antiviral innate immune responses in the lower respiratory tract involves TRAF3 and TRAF5 signaling. PMID: 26454171 TRAF3 regulates signaling to T cells not only through costimulatory members of the TNFR superfamily, but also through the T cell receptor complex, and cytokine receptors. PMID: 25723057 Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a positive regulator of pathological cardiac hypertrophy in mice and humans. PMID: 26034202 Focal genetic loss of TRAF3 is recurrent in human diffuse large B-cell lymphoma. PMID: 25468570 The role of TRAF3 and NIK in T cell malignancies is clarified. Results indicate that TRAF3 differentially governs the growth of B and T cell cancers. PMID: 24739416 It has become increasingly evident that the roles and functions of TRAF3 are highly context-dependent. TRAF3 can serve distinct roles for different receptors in the same cell, and also has highly cell-type-dependent functions. PMID: 24433987 After viral infection, HSCARG interacted with tumor necrosis receptor-associated factor 3 (TRAF3) and inhibited its ubiquitination by promoting the recruitment of OTUB1 to TRAF3. PMID: 24763515 SUMOylation is required for optimal TRAF3 signaling capacity. PMID: 24260396 a mechanistic basis for Traf3 alternative splicing and ncNFkappaB activation in contributing to T cell-dependent adaptive immunity. PMID: 24671418 These findings demonstrate that herpes simplex virus 1 ubiquitin-specific protease UL36 removes polyubiquitin chains on TRAF3 and counteracts the IFN-beta pathway. PMID: 23986588 noncanonical NF-kappaB activation by Tio depends on a distinct sequence motif that directly recruits tumor necrosis factor receptor-associated factor 3 (TRAF3). PMID: 23612708 TRAF3 and TRAF5 are overexpressed in inflammatory bowel disease. Although the endoscopic appearance can be normal, TRAF3 and TRAF5 pre-activation can be detected in non-inflamed colonic segments PMID: 23329887 In an attempt to explore TRAF3 binding partners that may be involved in TRAF3-regulated signaling, a yeast two-hybrid screen of a human spleen cDNA library was undertaken. RIP2 was identified as a TRAF3 binding partner. PMID: 23333941 Levels of TRAF3 degradation correlate with number of cells infected with Chlamydia pneumoniae, because more TRAF3 is detected at a lower multiplicity of C. pneumoniae infection. PMID: 23303668 model proposed where dsDNA and dsRNA sensing induces the formation of membrane-bound compartments originating from the Golgi, which mediate the dynamic association of TRAF3 with MAVS leading to an optimal induction of innate immune responses PMID: 22792062 Ubiquitination of NEMO negatively regulates the interferon antiviral response through disruption of the MAVS-TRAF3 complex. PMID: 22901541 A novel microRNA-32 mediated mechanism regulates TRAF3 in human microglial cells exposed to human immunodeficiency virus (HIV)-1 Tat protein. PMID: 22709905 TRAF3 functioned as ubiquitin E3 ligase for calcineurin and promoted its degradation. PMID: 22715070 Genetic lesions of the TRAF3 and MAP3K14 genes in classical Hodgkin lymphoma. PMID: 22469134 MIP-T3 is unique among cellular inhibitors of innate immunity because it appears to affect TRAF3 ubiquitination only slightly, but is capable of preventing TRAF3 from engaging downstream transducers and effectors. PMID: 22079989 TRAF3 participates in a number of the regulatory post-translational modifications involving ubiquitin that are important in various signaling pathways. PMID: 22017431 Data show that IKBalpha, NFKB2, and TRAF3 gene polymorphisms play a role in the development of multiple myeloma and in the response to bortezomib therapy. PMID: 21228035 Studies indicate that TRAF3 plays a highly versatile role of in immunity-related signal transduction. PMID: 21660053 Lack of TRAF3 expression might be one of the reasons for the aberrant expression of the unclassical NF-kappaB activity in Hodgkin's lymphoma cells. PMID: 19954665 In contrast to TRAF3 transgenic mice lacking TRAF3 in B cells, T cell TRAF3-deficient mice exhibit defective IgG1 responses to T-dependent antigen, as well as impaired T cell-mediated immunity to infection with Listeria monocytogenes. PMID: 21084666

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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