Recombinant Human Thyrotropin Subunit Beta (TSHB) Protein (His-SUMO)

Name: Recombinant Human Thyrotropin Subunit Beta (TSHB) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-09805P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Thyrotropin Subunit Beta (TSHB) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P01222
Target Symbol	TSHB
Synonyms	TSHBThyrotropin subunit beta; Thyroid-stimulating hormone subunit beta; TSH-B; TSH-beta; Thyrotropin beta chain; Thyrotropin alfa
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	FCIPTEYTMHIERRECAYCLTINTTICAGYCMTRDINGKLFLPKYALSQDVCTYRDFIYRTVEIPGCPLHVAPYFSYPVALSCKCGKCNTDYSDCIHEAIKTNYCTKPQKSY
Expression Range	21-132aa
Protein Length	Full Length of Mature Protein
Mol. Weight	28.9kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Indispensable for the control of thyroid structure and metabolism.
Subcellular Location	Secreted.
Protein Families	Glycoprotein hormones subunit beta family
Database References	HGNC: 12372 OMIM: 188540 KEGG: hsa:7252 STRING: 9606.ENSP00000256592 UniGene: Hs.406687

Gene Functions References

Serum macro TSH level is associated with sleep quality in patients with cardiovascular risks. PMID: 28287185
present the cases of two siblings with a novel mutation of TSHB. One of them was c.40A>G (rs10776792) which is a very common variation that is also seen in healthy individuals, the other was c.94G>A at codon 32 of exon 2 which resulted in a change from glutamic acid to lysine (p.E32K PMID: 28515030
The serum levels of IL-8, MIP-1 alpha, MIP-1 beta, MMP-8, Resistin, FLRG, and BCAM were significantly higher in breast cancer patients, but LAP and TSH-beta levels were lower. PMID: 26898119
results provide novel evidence of TSHB as a paracrine factor that is modulated in parallel with cholesterol metabolism in human adipose tissue PMID: 28646016
In hyperthyroidism, bone marrow resident macrophages have the potential to exert enhanced osteoprotective effects by oversecreting a TSH-beta splice variant. PMID: 27300765
In the context of metabolic syndrome, a higher TSH within the euthyroid range confers an increased leptin/adiponectin ratio, a proposed marker of atherosclerosis susceptibility and adipocyte dysfunction. PMID: 28077136
the distribution of 1(st) trimester TSH and evaluate its association with perinatal outcomes and future development of maternal thyrotoxicosis, is reported. PMID: 27351808
Data suggest that the subtle thyroid-stimulating hormone (TSH) reduction could possibly reflect minor hypothalamic-pituitary damage. PMID: 26432979
analysis of a single nucleotide substitution in TSHbeta related to clinical euthyroidism [family case report] PMID: 25950606
Findings defined the immune-derived functional TSHb splice variant that resided in the thyroid of patients with Hashimoto's thyroiditis (HT), which exerted the unique effects on the pathogenesis of the disease. PMID: 26170068
Serum TSH was associated with pulse wave velocity on population level when adjusted with age and sex. PMID: 25185682
Higher TSH levels are associated with less favorable lipid levels in children PMID: 25781359
In cases with isolated central congenital hypothyroidism and undetectably low TSH serum concentrations, a TSHbeta gene deletion should be considered PMID: 25012771
Epistasis between single nucleotide polymorphisms within the TSHB and ADAMTS16 genes may increase the risk of premature ovarian failure in Korean women. PMID: 24366283
High Thyroid Stimulating Hormone levels are associated with papillary thyroid carcinoma. PMID: 24595799
bTSH Induces IL-6 Protein and mRNA in Orbital Fibroblasts and Fibrocytes PMID: 24086448
The use of these age-specific reference intervals for TSH, especially in those over 70 years old, would result in the reclassification of many TSH results from "abnormal" to "normal" and avoid unnecessary treatment. PMID: 23345094
In Hashimoto's thyroiditis the hTSHbeta splice variant exists in human serum and dimerises with TSHalpha. PMID: 22752807
Data suggest that a progressive increase in TSH are predictive factors for thyroid failure in Hashimoto's thyroiditis (HT) patients. PMID: 21981142
Data suggest that likelihood of papillary thyroid carcinoma is reduced when serum TSH is lower, as in thyroid autonomy, and increased when serum TSH is higher, as in thyroid autoimmunity. [Meta-Analysis; REVIEW] PMID: 22278420
Studies indicate that immune system-derived TSH, in particular, a splice variant of TSHbeta that is preferentially made by cells of the immune system, is produced by a subset of hematopoietic cells that traffic to the thyroid. PMID: 20826543
Results suggest that a serum TSH concentration at the lower end of the reference range may be associated with low BMD in men. PMID: 20455884
Novel mutations of the TSH-beta subunit gene underlying congenital central hypothyroidism undetectable in neonatal TSH screening. PMID: 20534762
we describe two new sibships with isolated congenital central hypothyroidism due to two different homozygous TSHbeta gene mutations (c.Q49X and c.C105fs114X). PMID: 20553196
TSHbeta variants C105Vfs114X and Q49X are the most frequent cause of this severe disorder in Europe, now for the first time observed in compound heterozygous state. PMID: 15297803
Data suggest that the expression of Pit-1 in cells of the alpha SU-based gonadotropin cell lineage might also lead to the expression of growth hormone, prolactin, and thyroid-stimulating hormone beta subunit. PMID: 16133148
N-CoR and TRbeta cooperate in the regulation of the TSHbeta gene and this ligand-dependent repression is mediated by the LXXLL motif in N-CoR PMID: 16216492
Serum TSH is frequently suppressed after treatment with antithyroid drugs. PMID: 17954417
In a cohort of pregnant women without overt thyroid dysfunction, the risk of child loss increased with higher levels of maternal TSH. PMID: 19273570
Human pituitary, thyroid, and mononuclear leukocytes express a TSHbeta isoform that is analogous to the mouse TSHbeta isoform, consisting of a 27 nucleotide portion of intron 2 and all of exon 3, coding for 71.2% of the native human TSHbeta polypeptide. PMID: 19364510

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.