Recombinant Human Thyrotropin Receptor (TSHR) Protein (His)

Name: Recombinant Human Thyrotropin Receptor (TSHR) Protein (His)
Brand: Beta LifeScience
SKU: BLC-00867P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Thyrotropin Receptor (TSHR) Protein (His) is produced by our Baculovirus expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P16473
Target Symbol	TSHR
Species	Homo sapiens (Human)
Expression System	Baculovirus
Tag	C-10His
Target Protein Sequence	GMGCSSPPCECHQEEDFRVTCKDIQRIPSLPPSTQTLKLIETHLRTIPSHAFSNLPNISRIYVSIDVTLQQLESHSFYNLSKVTHIEIRNTRNLTYIDPDALKELPLLKFLGIFNTGLKMFPDLTKVYSTDIFFILEITDNPYMTSIPVNAFQGLCNETLTLKLYNNGFTSVQGYAFNGTKLDAVYLNKNKYLTVIDKDAFGGVYSGPSLLDVSQTSVTALPSKGLEHLKELIARNTWTLKKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILESLMCNESSMQSLRQRKSVNALNSPLHQEYEENLGDSIVGYKEKSKFQDTHNNAHYYVFFEEQEDEIIGFGQELKNPQEETLQAFDSHYDYTICGDSEDMVCTPKSDEFNPCEDIMG
Expression Range	21-413aa
Protein Length	Partial
Mol. Weight	48.7 kDa
Research Area	Neuroscience
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Plays a central role in controlling thyroid cell metabolism.
Subcellular Location	Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.
Protein Families	G-protein coupled receptor 1 family, FSH/LSH/TSH subfamily
Database References	HGNC: 12373 OMIM: 275200 KEGG: hsa:7253 STRING: 9606.ENSP00000298171 UniGene: PMID: 29290039 TSHR mutations occur in approximately 5% thyroid nodules in a large consecutive series with indeterminate cytology. TSHR mutations may be associated with an increased cancer risk when present at high allelic frequency PMID: 29516685 This work is devoted to the ascertainment of serological cross-reactivity between OmpF porin from Yersinia pseudotuberculosis (YpOmpF) and human thyroid-stimulating hormone receptor (hTSHR). PMID: 29079441 Genetic polymorphisms of CTLA-4 gene on the nucleotide 49 at codon 17 of exon 1, TSHR gene SNP rs2268458 of intron 1, number of regulatory T cells and TRAb levels play a role as risk factors for relapse in patients with Graves' disease. PMID: 29093229 Overexpression of TSHR was found in a great majority of hepatocellular carcinomatissues and associated with unfavorable prognosis PMID: 29715101 Signaling dissection using diverse inhibitors indicated that EOC cell proliferation driven by thyrostimulin-TSHR signaling is PKA independent, but does require the involvement of the MEK-ERK and PI3K-AKT signal cascades, which are activated mainly via the trans-activation of EGFR PMID: 27273257 SNPs rs179247 and rs12101255 were significantly associated with Graves disease. rs12101255 and rs2268458 polymorphisms had no association with Graves diseases and Graves ophthalmopathy. PMID: 27456991 Among the evaluated TSHR gene SNPs, the rs4411444 GG genotype and the rs4903961 C allele in the enhancer regions of the TSHR gene were most strongly associated with the development of Graves disease, especially intractable disease, and that of Hashimoto disease, respectively. PMID: 27762730 Low expression of TSHR is associated with dilated cardiomyopathy and impaired left ventricular function accompanied by increased risk of death. PMID: 29320567 Monoallelic TSHR mutations are significantly associated with positive newborn screen for congenital hypothyroidism, and the association is further strengthened by the coexistence of monoallelic DUOX2 mutations. PMID: 29092890 The role of TSH Receptor cleavage into subunits and shedding of the A-Subunit in Graves' disease pathogenesis is reviewed. PMID: 27454362 a hot-spot mutation in EZH1 is the second most frequent genetic alteration in autonomous thyroid adenomas; the association between EZH1 and TSHR mutations suggests a 2-hit model for the pathogenesis of these tumors, whereby constitutive activation of the cAMP pathway and EZH1 mutations cooperate to induce the hyperproliferation of thyroid cells PMID: 27500488 Germline mutation in the thyrotropin receptor gene is associated with non-autoimmune hyperthyroidism. PMID: 28195550 Congenital Hypothyroidism With Gland-in-Situ 59% attributable to mutations in TSHR. PMID: 27525530 data support the association that low intrathymic TSHR expression is associated with susceptibility to developing pathogenic TSHR antibodies, while high intrathymic TSHR expression is protective PMID: 28099999 Graves' disease is associated with polymorphisms of TSHR intron 1 rs179247 and rs12101255. There is no association between rs179247 SNPs and Graves' ophthalmopathy PMID: 27465319 TSHR gene mutations p.R528C and c.392+4del4 associated with congenital hypothyroidism. PMID: 26864598 this study shows that the TSHR is not main factor contributing to determining common genetic basis among Graves disease and alopecia areata PMID: 27810496 TSHR D727E polymorphism might be involved in the pathogenesis of toxic multinodular goiter PMID: 27525921 lncRNA PVT1 may contribute to tumorigenesis of thyroid cancer through recruiting EZH2 and regulating TSHR expression. PMID: 26427660 experimentally verified contact of Ser-281 (ECD) and Ile-486 (TMD) was subsequently utilized in docking homology models of the ECD and the TMD to create a full-length model of a glycoprotein hormone receptor PMID: 27129207 TSHR gene polymorphisms are associated with typical symptoms in primary congenital hypothyroidism. PMID: 26356361 The eminent ratio of TSHr methylation in well-differentiated thyroid carcinoma against benign thyroidal nodules adduced that TSHr methylation status can be utilized as a tumor marker for well-differentiated thyroid cancer. PMID: 26519197 Thyroid-stimulating hormone receptor intronic polymorphisms are associated with the susceptibility to Graves' disease and Graves' ophthalmopathy in the Brazilian population, but do not appear to influence the disease course. PMID: 25543543 data support a structural model of the TSHR transmembrane domain with a bulged transmembrane helix TM2 and a straight TM5 that is specific of glycoprotein hormone receptors PMID: 26545118 TSHR gene variants are associated with congenital hypothyroidism. PMID: 25153578 the hinge region and its adjacent domains have roles in binding and signaling patterns of the thyrotropin and follitropin receptor PMID: 25340405 subclinical hypothyroidism in heterozygotes with TSHR mutations is a stable compensated condition with an appropriately adjusted set point for pituitary-thyroid feedback that does not require replacement therapy PMID: 25557138 Patients with preoperative TSHR-mRNA >/=1.02 ng/mug may be at a greater risk for recurrence of thyroid cancer. PMID: 26212344 The present study demonstrates that the SmartAmp2 method is useful to detect the R450H mutation in TSHR. PMID: 24895636 The aim of this study was to investigate whether chimeric TSH receptor based bioassay can predict Graves' disease remission/relapse after antithyroid drug PMID: 24968734 TSHR polymorphisms were not associated with the dose of T4 or central obesity among hypothyroid patients. PMID: 25079464 TSHR mutations are common among Hungarian patients with Congenital hypothyroidism. The novel genetic alterations revealed an important structural role of the N432(1.50) and the P449(2.39) residues in receptor expression and signaling, respectively. PMID: 25978107 c.317+1G>A splice site mutation in the TSHR gene leads to sever congenital hypothyroidism PMID: 24859513 Our data suggest a role for PTCSC2, FOXE1, and TSHR in the predisposition to papillary thyroid carcinoma. PMID: 25303483 germline polymorphisms of TSHR do not confer susceptibility for the development of autonomously functioning thyroid nodules. PMID: 24789540 our study showed a high implication of TSHR gene methylation and its significant association with BRAF V600E mutation in thyroid tumors, depicting a positive connection between TSHR pathway and MAP Kinase pathway. PMID: 24927793 Allele A of the rs179247 polymorphism in the TSHR gene is associated with lower risk of GO in young GD patients. PMID: 25061884 study reports a large deletion of the TSHR gene in two siblings with congenital hypothyroidism coming from a consanguineous Turkish family PMID: 24690939 A gain-of-function mutation (M435R) activates the Gs/adenylyl cyclase pathway, deactivates the Gq/11-phospholipase C pathway, and causes nonautoimmune hyperthyroidism in a Japanese family. PMID: 24608569 This review covers several mutations in TSHR which are clearly associated with a hyperthyroidism-phenotype, but interestingly show a lack of constitutive activity determined by in vitro characterization. PMID: 24845969 A nonsense thyrotropin receptor gene mutation is associated with congenital hypothyroidism and heart defects. PMID: 24945425 A newly discovered TSHR mutation L665F in transmembrane helix 7 of the receptor was detected in six members of a family with hyperthyroidism. PMID: 24947036 findings demonstrate TSH-R expression is thymus-specific within the immune system; data support the notion of a novel neuroendocrine-immune interaction in which TSH-R signaling in the thymus, most likely mediated by TSH, enhances thymic T-cell development PMID: 24635198 Autonomous adenomas are caused by somatic mutations of the thyroid-stimulating hormone receptor in children. PMID: 24480816 genetic association studies in population in Japan: Data suggest that patients with TSHR mutations exhibit persistent hyperthyroidism throughout follow-up; in such patients, hyperthyroidism progresses despite treatment with antithyroid drugs. PMID: 24279482 Significant reduction in TSHR messenger RNA is associated with ovarian carcinomas. PMID: 24844218 discovered a genetic-epigenetic interaction involving a noncoding SNP in the TSHR gene that regulates thymic TSHR gene expression and facilitates escape of TSHR-reactive T cells from central tolerance, triggering Graves disease PMID: 25122677 The expression of TSHR and NIS genes is differently controlled by multiple mechanisms, including epigenetic events elicited by major signaling pathways involved in thyroid tumorigenesis. PMID: 24353283 Only the IL1RN tandem repeats polymorphism may be associated with Hashimoto's thyroiditis susceptibility; TSHR and IL1RN polymorphisms may represent prognostic factors for predicting the severity of the disease. PMID: 24328419

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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Recombinant Human Thyrotropin Receptor (TSHR) Protein (His)

Recombinant Human Thyrotropin Receptor (TSHR) Protein (His)

Product Overview

Target Details

FAQs