Recombinant Human TDGF1 Protein (C-Fc)

Name: Recombinant Human TDGF1 Protein (C-Fc)
Brand: Beta LifeScience
SKU: BL-0019NP
Availability: InStock

BL-0019NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

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Product Overview

Description	Recombinant Human Teratocarcinoma-derived Growth Factor 1 is produced by our Mammalian expression system and the target gene encoding Leu31-Ser169 is expressed with a human IgG1 Fc tag at the C-terminus.
Accession	P13385
Synonym	Teratocarcinoma-derived growth factor 1;Cripto-1 growth factor;CRGF;Epidermal growth factor-like cripto protein CR1;TDGF1;CRIPTO
Gene Background	Teratocarcinoma-derived growth factor 1(TDGF1) is a Cell membrane protein and contains 1 EGF-like domain. The protein plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. It also may play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Molecular Mass	42.8 KDa
Apmol Mass	40-60 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated TDGF1 acts as a Nodal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Subcellular Location	Cell membrane; Lipid-anchor, GPI-anchor. Secreted.
Protein Families	EGF-CFC (Cripto-1/FRL1/Cryptic) family
Database References	HGNC: 11701 OMIM: 187395 KEGG: hsa:6997 STRING: 9606.ENSP00000296145 UniGene: PMID: 29223130 Targeting the Cripto-1/TAK-1/NF-kappaB/Survivin pathway may be an effective approach to combat apoptosis resistance in cancer. PMID: 29807222 Knockdown of Cripto-1 inhibits the proliferation, migration, invasion, and angiogenesis in prostate carcinoma cells. PMID: 29358554 Results identified elevated CR-1 expression in esophageal squamous cell carcinoma (ESCC) specimens which correlated to poor prognosis of the patients. CR-1 high cells isolated from ESCC cells possess cancer stem-like cells (ECSLC) properties. PMID: 28431580 These results highlight a functional role for CRIPTO and GRP78 in prostate cancer metastasis and suggest that targeting CRIPTO/GRP78 signaling may have significant therapeutic potential. PMID: 28394345 PGAP6 plays a critical role in Nodal signaling modulation through CRIPTO shedding. PMID: 27881714 Upregulation of cripto-1 is associated with prostate cancer. PMID: 28098905 the miR-15b expression is negatively associated with the cripto-1 expression in glioma cells. miR-15b may subsequently impair growth and invasion of glioma cells through targeted regulation of cripto-1. PMID: 27082313 These findings suggest that CRIPTO expression may be a useful serological marker for diagnostic and/or prognostic purposes during germ cell cancer management. PMID: 26654129 serum CR-1 is a useful diagnostic and prognostic marker for nonsmall cell lung cancer patients. PMID: 26109366 Cripto-1 overexpression contributes to aggressiveness and poor prognosis of hepatocellular carcinoma PMID: 26375669 The main signaling pathways mediating Cripto-1 effects include Nodal-dependent (Smad2/3) and Nodal-independent (Src/p44/42/Akt) signaling transduction pathways PMID: 26327334 Cripto-1 is a novel and dynamically regulated effector of stem cell functions in colorectal cancer. PMID: 26343543 these findings suggest a novel molecular network, involving CRIPTO, AKT, and FGFR signaling, in favor of the emergence of mesenchymal-like cancer cells during the development of aggressive prostate tumors. PMID: 25596738 Report lower gene expression of Cripto in endometriosis but difference not maintained at protein level. PMID: 25228630 Treatment of U-87 MG cells with CR-1 leads to higher expression of drug efflux protein MDR-1 in the CR-1 positive subpopulation, indicating correlated induction of these two proteins. PMID: 25658584 These findings clearly suggest that the downregulation of miR-15a-16 with Cripto amplification may be involved in the development of nonsmall cell lung cancer. PMID: 24500260 Snail represses Cripto-1 gene by direct transcriptional interaction PMID: 25889638 CR-1 expression in glioblastoma multiforme(GBM)tissue and blood; CR-1 plasma levels from GBM patients were elevated compared with normal; CR-1 concentrations higher than normal correlated with shorter overall survival; identified CR-1 in different areas of GBM tissue including perivascular tumor cells and endothelial cells PMID: 24521322 the functional analysis of the main associated locus identified a causal variant in the 5'UTR of CRIPTO gene which is able to strongly modulate CRIPTO expression through an AP-1-mediate transcriptional regulation. PMID: 25629528 Our findings suggest that high Cripto-1 expression might be involved in the development of bladder cancer and a potentially effective prognostic marker in bladder cancer patients PMID: 25326807 Over expression of Cripto-1 was correlated with the occurrence, development, metastasis and malignant degree of lung adenocarcinoma. PMID: 25796148 these findings identify the CRIPTO/GRP78 pathway as a developmentally conserved regulator of fetal and adult mammary stem cell behavior ex vivo, with implications for the stem-like cells found in many cancers. PMID: 24749068 This study demonstrated CR-1 expression in the placental bed, its increased expression in creta placentas, and EVT cells as the main CR-1-producing cell type. PMID: 25165718 Data suggest that the high expression of Cripto-1 (CR-1) may play an important role in the progression of non-small cell lung cancer (NSCLC), and CR-1 expression may offer a valuable marker for predicting the outcome of patients with NSCLC. PMID: 24870591 Tumors from NSCLC patients with EGFR-activating mutations that were intrinsically resistant to EGFR-TKIs expressed higher levels of CRIPTO1 compared with tumors from patients that were sensitive to EGFR-TKIs. PMID: 24911146 Cripto is overexpressed in colonic neoplasms and is related to cancer cell migration/invasion. PMID: 24379580 The significance of cell surface CR-1 expression in human melanoma cells, was examined. PMID: 23574716 Cross-talk between Cripto-1 and the Wnt/beta-catenin signaling pathway might play a role in mammary transformation leading to a more aggressive behavior of mammary cancer cells. PMID: 23022962 Offer some insight into the transcriptional regulation of CR-1 gene expression and its critical role in the pathogenesis of human cancer. PMID: 23129342 human testicular tumors showed upregulation of NODAL and CRIPTO that was proportional to invasiveness and to the number of malignant cells. PMID: 23034635 Cripto/GRP78 modulates the TGF-beta pathway in development and oncogenesis [review] PMID: 22306319 Cripto-1 may play a role during developmental EMT, and it may also be involved in the reprogramming of differentiated tumor cells into cancer stem cells through the induction of an EMT program. PMID: 22542493 Cripto-1 plays a role in the malignant transformation of oral mucosa and is involved in the tumorigenesis and progression of oral squamous cell carcinoma by promoting the growth and migration of malignant cells. PMID: 21824804 High CRIPTO-1 is associated with cutaneous melanoma. PMID: 21863025 Disturbed expression of endometrial activin A, cripto , and follistatin suggests a dysfunction of the activin pathway in endometriosis/endometrioma. PMID: 21496809 work firstly provides human genetic evidence of TDGF1 involved in the pathogenesis of ventricular septal defects PMID: 19853938 expression of CR-1 may alter the physiochemical properties of the plasma membrane resulting in an enhancement of intercellular transfer of cellular signaling components which may account for the paracrine activity of CR-1. PMID: 21055389 Results demonstrate that CR-1 expression is enriched in an undifferentiated, tumorigenic subpopulation and is regulated by key regulators of pluripotent stem cells. PMID: 20549704 molecular model of activin receptor-like kinase 4/Cripto/Nodal complex built by homology modeling as well as docking tests aimed at identifying potential binding epitopes PMID: 20629020 TDGF1 has a role in metachronous metastasis of colorectal cancer PMID: 20126975 TDGF-1, which is significantly upregulated in APA and mediates aldosterone hypersecretion and deregulated growth in adrenocortical cells in vitro, may represent a key player in the development and pathophysiology of primary aldosteronism. PMID: 20385969 Nodal and Cripto immunoreactivity increased dramatically in the transition from histologic Grade 1 to histologic Grades 2 and 3 endometrial carcinomas. PMID: 19874624 Cripto has dual roles as a coreceptor as well as a coligand for Nodal and that this signaling interaction with Nodal is regulated by an unusual form of glycosylation. PMID: 12052855 A loss-of-function mutation in the CFC domain of TDGF1 is associated with human forebrain defects. PMID: 12073012 complexation with activin and type II activin receptors and role in blocking activin signaling PMID: 12682303 CRIPTO signaling promotes cardiomyogenesis and redirects the neural fate of embryonic stem cells. PMID: 14581455 Results suggest that cripto-1 overexpression might be associated with the progression towards a more aggressive phenotype in breast carcinoma, through the activation of both Akt and Smad-2 signalling pathways. PMID: 14584041 The regulation of Netrin-1 expression is important in regulating Cripto-1-dependent invasion and migration of mammary epithelial cells. PMID: 16176936 The plasma CR-1 might represent a novel biomarker for the detection of breast and colon carcinomas. A statistically significant increase in the levels of plasma CR-1 was found in patients with colon carcinoma and in patients with breast carcinoma. PMID: 16951234

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

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Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

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