Recombinant Human Tata Box-Binding Protein-Like Protein 1 (TBPL1) Protein (His-SUMO)

Name: Recombinant Human Tata Box-Binding Protein-Like Protein 1 (TBPL1) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-10104P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Product Overview

Description	Recombinant Human Tata Box-Binding Protein-Like Protein 1 (TBPL1) Protein (His-SUMO) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P62380
Target Symbol	TBPL1
Synonyms	21 kDa TBP-like protein; 21kDa TBP like protein; Second TBP of unique DNA; Second TBP of unique DNA protein; STUD; TATA box binding protein like protein 1; TATA box binding protein-related factor 2; TATA box-binding protein-like protein 1; TATA box-binding protein-related factor 2; TBP like 1; TBP like protein; TBP related factor 2; TBP-like factor; TBP-like protein 1; TBP-related factor 2; TBP-related protein; tbpl1; TBPL1_HUMAN; TLF; TLP; TRF2
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	MDADSDVALDILITNVVCVFRTRCHLNLRKIALEGANVIYKRDVGKVLMKLRKPRITATIWSSGKIICTGATSEEEAKFGARRLARSLQKLGFQVIFTDFKVVNVLAVCNMPFEIRLPEFTKNNRPHASYEPELHPAVCYRIKSLRATLQIFSTGSITVTGPNVKAVATAVEQIYPFVFESRKEI
Expression Range	1-185aa
Protein Length	Partial
Mol. Weight	36.8kDa
Research Area	Epigenetics And Nuclear Signaling
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Part of a specialized transcription system that mediates the transcription of most ribosomal proteins through the 5'-TCT-3' motif which is a core promoter element at these genes. Seems to also mediate the transcription of NF1. Does not bind the TATA box.
Subcellular Location	Cytoplasm. Nucleus.
Protein Families	TBP family
Database References	HGNC: 11589 OMIM: 605521 KEGG: hsa:9519 STRING: 9606.ENSP00000237264 UniGene: Hs.486507
Tissue Specificity	Ubiquitously expressed, with highest levels in the testis and ovary.

Gene Functions References

TLP Prevents p53 Degradation through Disrupting the p53-MDM2 Interaction. PMID: 28082682
The interaction of TBP-like protein with TFIIA is essential to suppress proteasome-dependent turnover of TBP-like protein PMID: 27696626
miR18a exhibits a protective role in colorectal cancer (CRC) via inhibiting proliferation, invasion and migration of CRC cells by directly targeting the TBPL1 gene. PMID: 26398009
results suggest that activation of the p21 upstream promoter is mediated by interaction between specific regions of TLP and p53 PMID: 24594805
Western blotting and immunohistochemistry both indicated a higher TBPL1 protein expression level in colorectal cancer tissue. siRNA for TBPL1 transfection obviously slowed down the cell proliferation in both SW-620 and HT-29 cells. PMID: 24870791
TLP mediates p53-governed transcriptional activation of the p21 upstream promoter. PMID: 22511763
Data show that during interphase, endogenous or exogenously expressed TRF2 is located almost exclusively in the nucleolus PMID: 15269281
TLF is a functional regulator of transcription with targets distinct from those of TBP. PMID: 15767669

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.