Recombinant Human T-Cell Immunoglobulin And Mucin Domain-Containing Protein 4 (TIMD4) Protein (His)

Name: Recombinant Human T-Cell Immunoglobulin And Mucin Domain-Containing Protein 4 (TIMD4) Protein (His)
Brand: Beta LifeScience
SKU: BLC-07839P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human T-Cell Immunoglobulin And Mucin Domain-Containing Protein 4 (TIMD4) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q96H15
Target Symbol	TIMD4
Synonyms	T-cell immunoglobulin mucin receptor 4 (TIM-4) (T-cell membrane protein 4)
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-10His
Target Protein Sequence	ETVVTEVLGHRVTLPCLYSSWSHNSNSMCWGKDQCPYSGCKEALIRTDGMRVTSRKSAKYRLQGTIPRGDVSLTILNPSESDSGVYCCRIEVPGWFNDVKINVRLNLQRASTTTHRTATTTTRRTTTTSPTTTRQMTTTPAALPTTVVTTPDLTTGTPLQMTTIAVFTTANTCLSLTPSTLPEEATGLLTPEPSKEGPILTAESETVLPSDSWSSVESTSADTVLLTSKESKVWDLPSTSHVSMWKTSDSVSSPQPGASDTAVPEQNKTTKTGQMDGIPMSMKNEMPISQ
Expression Range	25-314aa
Protein Length	Partial
Mol. Weight	37.4 kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Protein Families	Immunoglobulin superfamily, TIM family
Database References	HGNC: 25132 OMIM: 610096 KEGG: hsa:91937 STRING: 9606.ENSP00000274532 UniGene: PMID: 29208769 High expression of TIMD4 in clear cell renal cancer cells was closely related to short progression free survival time, and was associated with resistance to sorafenib. PMID: 28631038 VitD deficiency may contribute to the pathogenesis of allergic rhinitis (AR) by increasing the TIM4 expression. The results suggest that to regulate the serum calcitriol levels and the expression of VDR in DCs may be necessary to be taken into account in the treatment of AR. PMID: 28160341 This study aimed to investigate the association of two TIM-4 SNPs) with systemic lupus erythematosus (SLE)susceptibility in a Chinese Han population.The results imply that GG genotype of the TIM-4 gene at -1419 site might be associated with the disease activity of SLE. PMID: 28371471 Role of TIM-4 in exosome-dependent entry of HIV-1 into human immune cells PMID: 28740388 analysis of the molecular characteristics of both mTIM-4 and hTIM-4 provides a better understanding of the regions of the TIM-4 IgV domain critical for Ebola virus entry PMID: 27122575 Tim-4 expression is closely associated with glioma and may have a regulatory role PMID: 26741116 Data indicate that T cell immunoglobulin and mucin domain containing 4 (TIM-4) contributes, at least in part, to the pathogenesis of type 2 diabetes mellitus (T2D), possibly by regulating interleukin-1beta (IL-1beta). PMID: 25676395 TIM4 binds TIM3 on the surface of polarized Th1 cells to induce Th1 cell apoptosis, which may contribute to the development of Th2-dominant immune disorders. PMID: 26403707 TIM-4 rs7700944 and not TIM-1 rs41297579 G>A (-1454) is associated with rheumatoid arthritis(RA) in the present cohort of Egyptian and may be a risk factor for development of RA in Egyptian. PMID: 25899833 Data showed up-regulation of TIM-4 in lung cancer tissues and a correlation with poor prognosis. Also, TIM-4 was found to promote growth of lung cancer cells by its interaction with integrin alphavbeta3 through its RGD motif. PMID: 26512878 Tim-4 expression on monocytes and Tim-4 level in plasma were more highly increased in ankylosing spondylitis patients than in controls. PMID: 25359708 Our results do not support an association between the rs7700944 polymorphism of the TIM-4 gene and rheumatoid arthritis PMID: 24217665 Data suggest that genetic polymorphisms in ANGPTL3 (angiopoietin-like 3 protein), TIMD4 (T cell immunoglobulin mucin-4), and apolipoproteins A5 and B are among the genetic determinants of hypertriglyceridemia in Amerindian populations. [REVIEW] PMID: 24768220 TIM4 expression is promoted by Cockroach allergen Bla g 7 in dendritic cells leading to Th2 polarization PMID: 24204099 TIM-1 and TIM-4 are novel targets for ADAM10- and ADAM17-mediated ectodomain shedding. PMID: 24286866 FG-CC' siRNA blocking interaction of Tim-1 and Tim-4 can enhance dendritic cell vaccine activity against gastric cancer. PMID: 22709877 There were four SNPs in the promoter region of TIM4 in asthma patients of Chinese Han population, which were in linkage disequilibrium. PMID: 18704309 TIM-4 variant or a highly correlated nearby gene of the TIM-4 gene may play a critical role in the pathogenesis of rheumatoid arthritis in many ethnicities. PMID: 22353209 macrophage-derived TIM4 plays an important role in the induction of Tregs in gliomas, which may play an important role in tumor tolerance. PMID: 21896488 TIM-4 gene polymorphisms are associated with asthma in a Chinese Han population. PMID: 20727045 Overexpression of TIM-4 on antigen- presenting cells in transgenic mice reduces the number of antigen-specific T cells that remain after immunization, resulting in reduced secondary T cell responses. PMID: 21037090 results showed that Tim-4 mRNA expression in peripheral blood mononuclear cells was significantly higher in system lupus erythematosus patients than in healthy controls, especially those patients in the active phase of disease. PMID: 20140011 The polymorphism of 8570G > A in TIM4 may be associated with allergic asthma in the population of Han nationality from Hubei province of China. PMID: 17407086 The results demonstrate that Staphylococcus aureus derived Staphylococcal enterotoxin B promotes the TIM4 production in human dendritic cells. PMID: 17439824 TIM-4 and TIM-1 are immunologically restricted members of the group of receptors whose recognition of PS is critical for the efficient clearance of apoptotic cells and prevention of autoimmunity. PMID: 18082433 Structures of T cell immunoglobulin mucin protein 4 show a metal-Ion-dependent ligand binding site where phosphatidylserine binds. PMID: 18083575 Data show that the transmembrane region and cytoplasmic tail of TIM-4 are dispensable for apoptotic cell engulfment, and suggest that TIM-4 is a PtdSer tethering receptor without any direct signaling of its own. PMID: 19217291 TIM4 -1419G>A polymorphism might be the genetic factor for the risk of childhood asthma in Chinese Han population. PMID: 19392790

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.