Recombinant Human T-Box Transcription Factor Tbx5 (TBX5) Protein (His&Myc)

Name: Recombinant Human T-Box Transcription Factor Tbx5 (TBX5) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-07417P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human T-Box Transcription Factor Tbx5 (TBX5) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q99593
Target Symbol	TBX5
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	MADADEGFGLAHTPLEPDAKDLPCDSKPESALGAPSKSPSSPQAAFTQQGMEGIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMELHRMSRMQSKEYPVVPRSTVRQKVASNHSPFSSESRALSTSSNLGSQYQCENGVSGPSQDLLPPPNPYPLPQEHSQIYHCTKRKEEECSTTDHPYKKPYMETSPSEEDSFYRSSYPQQQGLGASYRTESAQRQACMYASSAPPSEPVPSLEDISCNTWPSMPSYSSCTVTTVQPMDRLPYQHFSAHFTSGPLVPRLAGMANHGSPQLGEGMFQHQTSVAHQPVVRQCGPQTGLQSPGTLQPPEFLYSHGVPRTLSPHQYHSVHGVGMVPEWSDNS
Expression Range	1-518aa
Protein Length	Full Length
Mol. Weight	65.2 kDa
Research Area	Cardiovascular
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	DNA-binding protein that regulates the transcription of several genes and is involved in heart development and limb pattern formation. Binds to the core DNA motif of NPPA promoter.
Subcellular Location	Nucleus. Cytoplasm.
Database References	HGNC: 11604 OMIM: 142900 KEGG: hsa:6910 STRING: 9606.ENSP00000309913 UniGene: PMID: 29972125 Study showed that thalidomide inhibited the TBX5/HAND2 physical interaction, and the in silico docking revealed that the same amino acids involved in the interaction of TBX5 with DNA are also involved in its binding to HAND2. Results establish a HAND2/TBX5 pathway implicated in heart development and diseases. PMID: 28469241 down-regulation of miR-10a-5p promotes proliferation and restricts apoptosis via targeting TBX5 in inflamed synoviocytes. PMID: 29545315 Diagnosis of Holt-Oram syndrome is possible as early as in prenatal period and it can be verified by genetic tests. PMID: 27958623 TBX5 overexpression markedly suppressed in vitro NSCLC cell proliferation, colony formation, and invasion and induced apoptosis. PMID: 28276311 TBX5 variants show possible pathogenic Fibrosis of the Cardiac Conduction system. PMID: 27005929 TBX5 deficiency-mediated downregulation of NFAT3 is crucial for the high cytokine-producing activity of T cells PMID: 29180489 A novel heterozygous missense mutation in TBX5 gene identified in a case of ventricular septal defect. The mutation causes significant changes of the activity of TBX5 in vitro. PMID: 28434921 There is no difference in NKX2.5 and TBX5 gene mutations between in vitro fertilization and naturally conceived children with congenital heart disease (CHD). PMID: 28606231 Rs7312625 of TBx5 gene was significantly associated with lone atrial fibrillation, and snp-snp interaction increased the risk of atrial fibrillation. PMID: 27479212 Hence, the variant distribution of NKX2-5, GATA4 and TBX5 are tightly associated with particular Congenital heart disease subtypes. Further structure-modelling analysis revealed that these mutated amino acid residuals maintain their DNA-binding ability and structural stability PMID: 27426723 Findings suggest that a single introduction of the three cardiomyogenic transcription factor (GATA4, cand TBX5)genes using polyethyleneimine (PEI)-based transfection is sufficient for transdifferentiation of adipose-derived stem cells (hADSCs) towards the cardiomyogenic lineage. PMID: 27553283 Three genes-ATP6V1G1 in 9q32, GMPS in 3q25.31, and TBX5 in 12q24.21-exhibited concomitant hypermethylation and decreased expression. The i(12p)-positive cells displayed global hypomethylation of gene-poor regions on 12p, a footprint previously associated with constitutional and acquired gains of whole chromosomes as well as with X-chromosome inactivation in females PMID: 26890086 report on the association between a TBX5 lossoffunction mutation and increased susceptibility to atrial fibrillation PMID: 27035640 Whole-genome microarray mRNA-expression profiles of myofibroblasts and skin fibroblasts revealed four additional genes that are significantly differentially expressed in these two cell types: NKX2-3 and LRRC17 in myofibroblasts and SHOX2 and TBX5 in skin fibroblasts PMID: 27036009 The Barrett-associated variants at GDF7 and TBX5 also increase esophageal adenocarcinoma risk. PMID: 26783083 These include a patient with hypoplastic phalanges and absent hallux bilaterally with de novo deletion of 11.9 Mb on 7p21.1-22.1 spanning 63 genes including RAC1, another patient with severe Holt-Oram syndrome and a large de novo deletion 2.2 Mb on 12q24.13-24.21 spanning 20 genes including TBX3 and TBX5, and a third patient with acheiropodia who had a nullizygous deletion of 102 kb on 7q36.3 spanning LMBR1 PMID: 26749485 provide both genetic and functional evidence to support the contribution of TBX5 gene in the pathogenesis of Atrial fibrillation PMID: 26762269 In summary, our study implicated a gene network involving Tbx5, Osr1 and Pcsk6 interaction in second heart field for atrial septation, providing a molecular framework for understanding the role of Tbx5 in congenital heart disease ontogeny. PMID: 26744331 The findings expand the mutational spectrum of TBX5 linked to Atrial fibrillation (AF), and provide new evidence that dysfunctional TBX5 may contribute to lone AF. PMID: 26917986 TBX5 microdeletion with microinsertion was detected in patient with Holt-Oram syndrome. PMID: 26780237 The crystal structure of cardiac TBX5 protein includes the N-terminal and DNA binding domains, which mediate intermolecular interactions. PMID: 26926761 Exome analysis revealed the splice mutation (c.148-1G>C) in TBX5 gene showing that haploinsufficiency of TBX5 protein caused the symptoms of the patients with Holt-Oram syndrome. PMID: 26401820 defines a TBX5-nucleosome remodeling and deacetylase interaction essential to cardiac development and the evolution of the mammalian heart PMID: 26859351 Data show that the combination of GATA binding protein 4 (Gata4), T-box transcription factor 5 (Tbx5) and BRG1-associated factor 60C protein (Baf60c) is sufficient for inducing adipose tissue-derived mesenchymal stem cells (ADMSCs) to form cardiomyocytes. PMID: 26071180 All Holt-Oram syndrome patients in this study showed cardiac septal anomalies. Half of them showed TBX5 gene mutations. PMID: 25216260 a novel heterozygous TBX5 mutation, p.A143T, was identified in a patient with sporadic dilated cardiomyopathy. PMID: 25963046 TBX5 mutation is involved in the development of cardiac conduction disorders. PMID: 25426816 Two heterozygous mutations in TBX5 were discovered in screening a series of 94 patients with Tetralogy of Fallot. PMID: 25263169 A novel TBX5 mutation, p.S154A, was identified in a family with familial dilated cardiomyopathy.The mutation was associated with significantly decreased TBX5 transcriptional activity. PMID: 25725155 a novel mutation of TBX5 gene in Holt-Oram Syndrome PMID: 24408148 TBX5 isoforms derived from novel exons have distinct expression domains and function. Alternative splicing regulates TBX5 function in heart and limb. PMID: 25623069 TBX5 mutations and clinical features of Holt-Oram syndrome. [Review] PMID: 25680289 This is a novel gene implicated in atrial fibrillation PMID: 25124494 miR-10a and miR-10b repressed TBX5 expression and decreased TBX5 protein levels by targeting the TBX5 3'-untranslated region in Congenital heart disease patients . PMID: 24714979 closest protein-coding genes were respectively GDF7 (rs3072), which encodes a ligand in the bone morphogenetic protein pathway, and TBX5 (rs2701108), which encodes a transcription factor that regulates esophageal and cardiac development PMID: 25447851 Reprogramming efficiency was significantly enhanced for cardiac myocytes when there were lower levels of Tbx5. PMID: 25416133 Rheumatoid arthritis synovial fibroblasts contribute to the inflammatory processes operating in the pathogenesis of rheumatoid arthritis via epigenetic control of TBX5. PMID: 25320281 Data show that Hdac3 physically interacts with Tbx5 and modulates its acetylation to repress Tbx5-dependent activation of cardiomyocyte lineage-specific genes. PMID: 24565863 The TBX5 DNA-binding domain complex was shown to interact with a DNA element from atrial natriuretic factor. PMID: 24817716 Tbx5 knock-down hearts displayed a marked decrease in vascular density and coronary vasculogenesis. PMID: 25245104 Our results indicate that rs3825214 conferred a significant risk of lone AF in this Chinese Han population. PMID: 23717681 beta-catenin forms a complex with YAP1 and TBX5, which promotes colon cancer cell survival and contributes to malignant transformation. These observations reveal hitherto unidentified components of the beta-catenin pathway that play key roles in survival of beta-catenin-active cells. PMID: 23245941 Single-base-pair mutation in the TBX5 enhancer is associated with the isolated congenital heart disease. PMID: 22543974 This is the first known report of an intragenic duplication of TBX5 and its clinical effects; an atypical HOS phenotype. PMID: 22333898 data will not only deepen our understanding of genetic causes of CHD but also provide insight into designing novel personalized therapy for adult patients with CHD by upregulating TBX5 gene expression with different approache PMID: 22901678 We will introduce several genetic approaches, which have been or are currently being applied to the study of PDA, that have been successful in identifying polymorphisms associated with adult diseases. PMID: 22414880 Mutations of TBX5 cause Holt-Oram syndrome, which includes congenital heart disease as a clinical feature. PMID: 22011241 This incomplete penetrance has not been described in TBX5-associated Holt-Oram syndrome or in families with clinical Holt-Oram syndrome PMID: 21752519 Data show that Tbx4 and Tbx5 harbour conserved and divergent transcriptional regulatory domains that account for their roles in limb development. In particular, both factors share an activator domain and the ability to stimulate limb growth PMID: 20975709

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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