Recombinant Human Synaptosomal-Associated Protein 25 (SNAP25) Protein (His)

Name: Recombinant Human Synaptosomal-Associated Protein 25 (SNAP25) Protein (His)
Brand: Beta LifeScience
SKU: BLC-04384P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Synaptosomal-Associated Protein 25 (SNAP25) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P60880
Target Symbol	SNAP25
Synonyms	bA416N4.2; Bdr; CMS18; dJ1068F16.2; FLJ23079; HGNC:11132; MGC105414; MGC139754; Resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC-4; RIC4; SEC 9; SEC9; SNAP 25; SNAP; SNAP-25; SNAP-25B; SNAP25; SNP 25; SNP25; SNP25_HUMAN; sp; SUP; Super protein; Synaptosomal associated 25 kDa protein; Synaptosomal associated protein; Synaptosomal associated protein 25; Synaptosomal associated protein 25kDa; Synaptosomal-associated 25 kDa protein; Synaptosomal-associated protein 25; Synaptosomal-associated protein; Synaptosomal-associated protein, 25-KD
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLDRVEEGMNHINQDMKEAEKNLKDLGKCCGLFICPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG
Expression Range	1-206aa
Protein Length	Full Length of Isoform 2
Mol. Weight	27.3kDa
Research Area	Neuroscience
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Subcellular Location	Cytoplasm, perinuclear region. Cell membrane; Lipid-anchor. Cell junction, synapse, synaptosome. Photoreceptor inner segment.
Protein Families	SNAP-25 family
Database References	HGNC: 11132 OMIM: 600322 KEGG: hsa:6616 STRING: 9606.ENSP00000254976 UniGene: PMID: 29491473 report some evidence supporting the association of SNAP25 to Attention Deficit/Hyperactivity Disorder PMID: 26941099 Single nucleotide polymorphism in SNAP-25 gene is associated with Attention deficit hyperactivity disorder. PMID: 28512748 Findings indicate that lower SNAP-25 in ventromedial caudate in schizophrenia is primarily due to less SNAP-25A, and that the remaining SNAP-25 is associated with greater protein-protein interaction with syntaxin. The absence of a link to recent treatment effects in the human samples, or to administration of antipsychotic drugs in rats suggests the findings are illness- and not treatment-related. PMID: 26971072 Allelic variation of SNAP25 modulates development and plasticity of the prefrontal-limbic network and a shared genetic vulnerability between Bipolar Disorder and Schizophrenia. PMID: 28972123 SH3BP5, LMO3, and SNAP25 were expressed in diffuse large B-cell lymphoma cells and associated with clinical features. PMID: 27184832 show that FOXC1 regulates the expression of RAB3GAP1, RAB3GAP2 and SNAP25 PMID: 28575017 The results of this study suggested that the polymorphisms rs3746544 and rs1051312 may increase the odds of developing ADHD. PMID: 27380186 study demonstrated that miR-27a and -b, which are widely expressed in host cells, suppress SNAP25 and TXN2 expression through posttranscriptional gene silencing. PMID: 28356525 Data suggest that A-syn (alpha-synuclein) promotes SNARE-dependent vesicle docking; phosphatidylserine (PS) removal from t-SNARE-bearing vesicles causes A-syn to inhibit vesicle docking; PS removal from v-SNARE-bearing vesicles promotes vesicle docking; the C-terminal 45 residues of A-syn are required for promotion of vesicle docking. (Here, t-SNARE is SNAP-25; v-SNARE is VAMP2.) PMID: 28495859 our data indicate that the expression of SNAP25 is crucial for dendrite formation and is associated with the effects of targeted chemotherapy. The detection of SNAP25 expression in MB cells may thus be essential for the chemotherapeutic application of Ara-C. PMID: 28339008 Robust association of the rs3746544 SNP and ASD, in both allele and haplotype-based analyses, was observed in Iranian population. PMID: 27888397 Our results will provide novel evidence to reveal the possible role of SNAP-25 in B[a]P-induced neurotoxicity and may be helpful for searching the potential strategy for the prevention measures against B[a]P neurotoxicity. PMID: 28412278 Our analysis indicated that there is no significant association between none of studied variants in SNAP-25 and ADHD. PMID: 27627841 These results of this study suggested that SNAP25 and NOS1 genotypes influence ADHD symptoms only in adults with ADHD. PMID: 26821215 Snap25 rs363050 (G) allele, which results in a reduced expression of Snap25, is associated with altered glycemic parameters in T2DM possibly because of reduced functionality in the exocytotic machinery leading to suboptimal release of insulin. PMID: 26779543 single nucleotide polymorphisms in either the region of NEUROD6 or SNAP25 were significantly associated with Alzheimer's Disease, in APOE4+ females and APOE4+ males, respectively. PMID: 26395074 Data demonstrate a role for SNAP-25 in controlling PSD-95 clustering and open the possibility that genetic reductions of the protein levels may contribute to the pathology through an effect on postsynaptic function and plasticity. PMID: 25678324 BoNT-A injection, but not Lipotoxin instillation, effectively cleaves SNAP-25 in the suburothelium. PMID: 26241848 The expression of SNAP-25 within the enteric nervous system and its downregulation in DD suggest an essential role in enteric neurotransmission and as a marker for impaired synaptic plasticity in enteric neuropathies. PMID: 25655772 The SNAP-25 Ddel T/C genotype was more common in fibromyalgia syndrome patients compared to controls, and it related to behavioral symptoms, personality and psychological disorders. PMID: 24885975 The rs363050 gene polymorphism correlates with decreasing cognitive scores in autistic children. PMID: 25629685 This study demonstrated that SNAP-25 polymorphisms may be associated with Alzheimer's disease and correlate with alterations in categorical fluency and a reduced localized brain activity. PMID: 25024311 SNARE complex genes and their interactions may play a significant role in susceptibility and working memory of ADHD. PMID: 25445064 Patterns of the immunoreactivity with antibodies to SNAP-25, synapsin-I and synaptophysin are completely appropriate to those of adult's OB on the 38-40 weeks of the prenatal development. PMID: 26204769 The results show that the positively charged amino acids at the SNAP-25 C terminus promote tight SNARE complex zippering and are required for high release frequency and rapid release in individual fusion events. PMID: 25698757 Ile67Asn variant in SNAP25B is pathogenic because it inhibits synaptic vesicle exocytosis PMID: 25381298 Cerebrospinal fluid SNAP-25 differentiated Alzheimer's disease from controls PMID: 25418885 SNPs in SNAP25 represent a common risk factor of both schizophrenia and major depressive disorder in the Han Chinese population. PMID: 25650683 In conclusion,the present results of a family-based association study do not support a strong role of SNARE genes in adult ADHD PMID: 24176595 Full-length SNAP25 is cleaved more efficiently by the protease domain of serotype A botulinum neurotoxin than its shorter fragments. PMID: 24769566 found evidence of the association of SNAP25 and ADHD. PMID: 24362847 no associaton with idiopathic generalized epilepsy was found regarding Intron 7 rs1569061 of Syntaxin 1A gene, MnlI rs3746544 and DdeI rs1051312 polymorphisms of SNAP-25 gene compared with healthy subjects PMID: 24164654 Association of impulsivity and polymorphic microRNA-641 target sites in the SNAP-25 gene. PMID: 24391914 direct interaction between TRIM9 and the Netrin-1 receptor DCC as well as a Netrin-1-sensitive interaction between TRIM9 and the SNARE component SNAP25. The interaction with SNAP25 negatively regulates SNARE-mediated exocytosis and axon PMID: 24778312 A combination of NET1 (rs2242447) and SNAP-25 (rs3746544) is a risk factor for ADHD. PMID: 23872233 DNA variation at SNAP25 confers risk to attention deficit disorder with hyperactivity. PMID: 23593184 Significant associations between two SNPs in the SNAP-25 gene (rs363039 and rs363050) and shyness was found. PMID: 23888754 There was an association between schizophrenia and the SNAP-25 rs1503112 polymorphism found, which survived correction for multiple testing. PMID: 22940547 There was a trend towards the increase in the frequency of an G allele of SNAP-25 in siblings of patients. PMID: 23612411 syntaxin 1 and SNAP-25 cooperate as SNARE proteins to support neuron survival. PMID: 23403573 PRIP inhibits regulated exocytosis through the interaction of its C2 domain with syntaxin 1 and SNAP-25, potentially competing with accessory proteins such as synaptotagmin I and by directly inhibiting trans-SNARE complex formation PMID: 23341457 low IQ group of children has higher frequency of SNAP25 allele, associated with intellectual disability. PMID: 22762387 There was a loss of SNAP-25 in visual cortex in dementia with Lewy bodies compared to controls. PMID: 23242284 no Alzheimer's Disease-associated differences in SNAP25 promoter DNA methylation were observed PMID: 22732502 Data obtained in this preliminary study indicates that rs3746544 'T' SNAP25 allele may have some role in the disease etiology in the studied Indian population PMID: 21996783 SNAP25 might represent the first description of an adaptively evolving gene with a role in cognition. PMID: 22193912 Letter: incubation of pancreatic islets with fibronectin improves beta cell function via increased expression of syntaxin 1 and SNAP25. PMID: 21926557 Forming an acceptor SNARE complex between syntaxin-1A and SNAP-25 weakens but does not abrogate cholesterol-controlled cluster formation and indicates that the reconstitution process results in equal incorporation of protein at either lipid composition. PMID: 21916482 DdeI and MnII T/T genotypes of SNAP25 may be a risk factor for antisocial behavior in a Turkish population. PMID: 21756448

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Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

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