Recombinant Human Suppressor Of Cytokine Signaling 1 (SOCS1) Protein (His)

Name: Recombinant Human Suppressor Of Cytokine Signaling 1 (SOCS1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-03037P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Suppressor Of Cytokine Signaling 1 (SOCS1) Protein (His) is produced by our Yeast expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	O15524
Target Symbol	SOCS1
Synonyms	CISH 1; CISH1; Cytokine inducible SH2 protein 1; JAB; JAK binding protein; JAK-binding protein; Janus kinase binding protein ; SOCS 1; SOCS-1; Socs1; SOCS1_HUMAN; SSI 1; SSI-1; SSI1; STAT induced STAT inhibitor 1; STAT-induced STAT inhibitor 1; Suppressor of cytokine signaling 1; Supressor of cytokine signalling 1; TEC interacting protein 3; Tec-interacting protein 3; TIP 3; TIP-3; TIP3
Species	Homo sapiens (Human)
Expression System	Yeast
Tag	N-6His
Target Protein Sequence	MVAHNQVAADNAVSTAAEPRRRPEPSSSSSSSPAAPARPRPCPAVPAPAPGDTHFRTFRSHADYRRITRASALLDACGFYWGPLSVHGAHERLRAEPVGTFLVRDSRQRNCFFALSVKMASGPTSIRVHFQAGRFHLDGSRESFDCLFELLEHYVAAPRRMLGAPLRQRRVRPLQELCRQRIVATVGRENLARIPLNPVLRDYLSSFPFQI
Expression Range	1-211aa
Protein Length	Full Length
Mol. Weight	25.6kDa
Research Area	Immunology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Through binding to JAKs and IFNGR1, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway.
Subcellular Location	Nucleus. Cytoplasmic vesicle. Note=Detected in perinuclear cytoplasmic vesicles upon interaction with FGFR3.
Protein Families	SOCS1 family
Database References	HGNC: 19383 OMIM: 603597 KEGG: hsa:8651 STRING: 9606.ENSP00000329418 UniGene: PMID: 30453988 SOCS1 gene methylation caused gene silencing of SOCS1. PMID: 29916533 SOCS1 is regulated in prostate cancer cells through miR-221-5p expression. PMID: 29506516 The results indicate SOCS1 plays an important role in AML and its higher expression serves as a new biomarker to risk-stratify AML patients. PMID: 28753595 reveal a vital contribution of miR-155 upregulation and consequent SOCS1 downregulation to an immune response triggered via IFN-gamma in BA PMID: 28355202 Guar gum fiber increases SOCS-1 expression in Caco-2 cells. PMID: 28608623 overexpression of SOCS-1 prevented TNF-alpha-mediated cell oxidative stress and apoptosis in human renal tubular epithelial cells PMID: 28732559 we report here that SOCS1 impacts the pattern of secreted products in cells with active p53 and is required for the expression of a selective set of p53 target genes including those involved in ferroptosis. PMID: 29081404 SOCS1 attenuates migration and invasion properties of hepatocellular carcinoma cells at least partly via modulation of MET-mediated epithelial-mesenchymal transition, and controls invasive tumor growth. PMID: 29085209 Our findings revealed significantly higher rates of insulin resistance, obesity and dyslipidaemia in Turkish patients with polycystic ovary syndrome compared with age-matched healthy controls, while no difference between study groups in terms of the rate of SOCS1-1478 CA/del polymorphism along with no significant relation of SOCS1-1478 CA/del polymorphism to insulin resistance and PCOS status. PMID: 28569589 polymorphisms of the SOCS1 gene are associated with the risk of ischemic stroke in a northern Chinese Han population, suggesting that SOCS1 gene polymorphisms may play an important role in the pathogenesis of ischemic stroke PMID: 27216615 our findings demonstrate that 5-hmC loss is an epigenetic hallmark of hepatocellular carcinoma , and miR-29a is an important epigenetic modifier, promoting HCC metastasis through TET-SOCS1-MMP9 axis silencing. The results offer a new strategy for epigenetic cancer therapy PMID: 28661477 SOCS-1, SOCS-2, and SOCS-3 proteins may directly or indirectly, have important roles in development and pathogenesis of papillary thyroid cancer PMID: 28643757 Report simultaneous over-expression of miR-155 and SOCS1 that may be characteristic for Sjogren's syndrome. PMID: 26620207 SOCS1 inhibits epithelial IL-13 signalling, supporting its key role in regulating Th2-driven eosinophilia in severe asthma PMID: 27338192 SOCS1 antagonizes epithelial-mesenchymal transition by suppressing Src activity, leading to thioredoxin expression and down-regulation of ROS levels in colon cancer cells PMID: 27613835 Our findings support the tumor suppressor role of SOCS1 in PCa and suggest that attenuation of MET signaling is one of the underlying mechanisms. SOCS1 in PCa cells also appears to prevent the tumor-promoting functions of cancer-associated fibroblasts. PMID: 27779203 Findings indicate that miR-21 and miR-155 promote the development of non-small cell lung carcinoma (NSCLC) in part by downregulating three tumor suppressor genes suppressor of cytokine signaling 1 protein (SOCS1), SOCS6, and PTEN. PMID: 27811366 Evaluating SOCS1 and p21 protein expression in prostatectomy specimens may have a prognostic value in identifying the aggressive prostate cancer. Inverse correlation between SOCS1 expression and disease severity was observed. However, SOCS1 expression did not correlate with that of p53, MET or p21 in the whole study cohort. PMID: 28235401 In this study, the authors report that JOSD1 plays an important role in regulating type-I interferon (IFN-I)-mediated antiviral activity. JOSD1 physically interacts with SOCS1, which is an essential negative regulator of many cytokines signaling, and enhances SOCS1 stability by deubiquitinating K48-linked polyubiquitination of SOCS1. PMID: 28355105 Co-immunoprecipitation experiments showed that SOCS1 mutations abolished JAK3 binding, revealing a key role for SOCS1 in regulating JAK3/STAT5 signaling PMID: 27144517 C/EBPbeta overexpression or knockdown did not change the levels of IL-1beta-induced SOCS1. SOCS1 regulated the levels of C/EBPbeta mRNA by ubiquitination of C/EBPbeta as well as transcriptional regulation in human chondrocytes. PMID: 27339399 patients with renal graft rejection expressed lower levels of SOCS1 PMID: 27423763 Inhibiting the STAT3-Mcl-1 signaling axis by ectopic SOCS1 improved radiosensitivity by inducing apoptosis and enhancing DNA damage after radiotherapy, offering a mechanistic rationale for a new esophageal squamous cell carcinoma treatment. PMID: 29042418 an overview of the mechanisms underlying SOCS1 function as a tumor suppressor and discuss the emerging evidences of SOCS1 activity as an oncogene. PMID: 26811119 MiRNA sponges against miR-19 or miR-155 inhibit the functions of these miRNAs and potentiate the induction of SOCS1 and p53 in mouse leukemia cells and in human myeloma cells. PMID: 26841929 Mutations of CREBBP and SOCS1 are independent prognostic factors in diffuse large B cell lymphoma; SOCS1 mutations were associated with better progression-free survival. PMID: 28302137 These results suggest that miR146a may regulate SOCS1/STAT3 and cytokine signalling in monocytes, directing T-cell differentiation and balancing immune clearance and immune injury during chronic viral infection. PMID: 27004559 IFN-lambda signaling is regulated by SOCS1 but not by SOCS3 or USP18. PMID: 28900038 This study evaluation the roles of SOCS1, the regulator of TLR9, RIG-I, and CD152 in patients with liver fibrosis/cirrhosis; the use of polymorphisms as markers for genetic risk is reported. PMID: 28762092 SOCS1 rs33932899 genotype was significantly related to severe necroinflammatory activity (NIA) grade of chronic hepatitis C patients. PMID: 28704535 Studies indicate that suppressors of cytokine signaling (SOCS) proteins CIS, SOCS1, and SOCS3 can be considered the third immunocheckpoint molecules since they regulate cytokine signals that control the polarization of CD4(+) T cells and the maturation of CD8(+) T cells. PMID: 28188673 Results provide evidence that repression of SHP-1, SHP-2 and SOCS-1 gene expression in patient plasma cells supports the constitutive activation of the JAK/STAT3 pathway and probably leads to higher degrees of bone marrow invasion. PMID: 28369102 Suppressor of cytokine signaling 1 (SOCS1) expression was upregulated in sustained virological responses (SVR) patients compared with non-SVR patients based on a multiplex reverse transcription-polymerase chain reaction analysis. Peripheral blood SOCS1 expression before treatment was not associated with SVR in hepatitis C virus (HCV) genotype 1 patients treated with pegylated IFN-alpha and ribavirin. PMID: 26786212 tumor-suppressor functions of SOCS1 in the liver could be mediated, at least partly, via regulation of the expression, stability and subcellular distribution of p21 and its paradoxical oncogenic functions, namely, resistance to apoptosis and increased proliferation PMID: 26725321 Suppressor Of Cytokine Signaling 1 (SOCS1) overexpression in retinal pigment epithelium cells inhibits some IFNgamma-mediated responses that lead to uveitis development. This notion raises the possibility that SOCS1 overexpression could be a novel target for treating non-infectious uveitis. PMID: 27478014 nectin-4 serves as a stimulatory co-receptor for the prolactin receptor by regulating the feedback inhibition of SOCS1 in the JAK2-STAT5a signaling pathway PMID: 28258213 This study showed that SOCS5 expression was significantly down-regulated. PMID: 28196747 this study shows that IL-7 induces the expression of SOCS1 through the JAK/STAT-5 pathway PMID: 27423467 SOCS1 silencing can inhibit proliferation and induce apoptosis of MV3 and A357 melanoma cells in vivo and in vitro by inhibiting extracellular signal-regulated kinase and janus kinase signal transduction and activators of transcription signaling pathways. PMID: 28466787 SOCS1 may promote apoptosis of intestinal epithelial cells at least partly through mediating p53 signaling. PMID: 27236107 The results revealed that although the expression levels of SOCS1, SOCS3 and, in particular, pSHP2, tend to decrease in the four types of astrocytomas, PIAS3 downregulation is more negatively correlated with STAT3 activation in the stepwise progress of astrocytomas and would indicate an unfavorable outcome. PMID: 28035384 findings provide novel insight into how dysregulated expression of SOCS increases IL-4 responses in allergic monocytes, and this may represent a new therapeutic avenue for managing allergic disease. PMID: 27507812 Overexpression of SOCS1 has a potent antitumor effect against ESCC. PMID: 28233302 IL-4 signaling plays a crucial role in the regulatory functions of SOCS1 in apoptosis and inflammation in human osteoarthritic chondrocytes. These findings provide a potential therapeutic target for the clinical treatment of osteoarthritis (OA) PMID: 28373981 Through promoter hypermethylation, the SOCS-1 gene was severely down-regulated in the colorectal cancer tissue samples. PMID: 27133036 Reduced SOCS1 protein levels in systemic lupus erythematosus patients are correlated with enhanced STAT1 activation. PMID: 27781323 These data thus suggest that overexpression of SOCS1 and SOCS3 induced by denguevirus infection leads to impairment of antiviral response through the inhibition of STAT functionality. PMID: 26709547 miR-203 and miR-221 regulate SOCS1 and SOCS3 in essential thrombocythemia PMID: 26990535 The HBVX gene upregulated the mRNA and protein expression levels of DNMT3A/3B, downregulated the expression of SOCS1 and increased SOCS1 gene promoter CpG island methylation. PMID: 26573490

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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Recombinant Human Suppressor Of Cytokine Signaling 1 (SOCS1) Protein (His)

Recombinant Human Suppressor Of Cytokine Signaling 1 (SOCS1) Protein (His)

Product Overview

Target Details

FAQs