Recombinant Human Soluble DLK-1 (sDLK-1) Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0008UL
Recombinant Human sDLK-1 (2 μg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band around 40-45 kDa, and purity greater than 98%.
Recombinant Human sDLK-1 (2 μg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band around 40-45 kDa, and purity greater than 98%.

Recombinant Human Soluble DLK-1 (sDLK-1) Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0008UL
/
Size

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Product Overview

Product Name Recombinant Human Soluble DLK-1 (sDLK-1) Protein
Target Symbol DLK1
Alternative Names FA1 (Fetal Antigen 1)
Accession Number P80370.3
Expression System HEK293 cell line
Molecular Weight 29.9 kDa (monomer, predicted), 40-45 kDa (observed)
Sequence Ala24-Ala304
Purity >98% under reducing conditions by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining
Activity Determined by an inhibition of adipogenesis differentiation using 3T3-L1 cells (Smas, C.M. et al. (1993) Cell73:725). There is >40% inhibition of differentiation as measured by Oil Red O staining with 10-100 ng/mL sDLK-1 treatment.
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level <0.001 EU per 1 μg of the protein as determined by the LAL method
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 μg/mL in PBS. Optionally, use PBS with 0.1% BSA.
Target Function The soluble form of human Delta-like 1 homolog (sDLK-1) is a circulating glycoprotein that primarily acts as a master regulator of cell differentiation and metabolic adaptation. sDLK-1 generally functions to maintain stem and progenitor cells in an undifferentiated, proliferative state. It is a well-characterized inhibitor of several mesodermal differentiation pathways. sDLK-1 functions as an endocrine signaling molecule, particularly during periods of high metabolic demand like pregnancy or early development. Unlike its typical inhibitory roles, soluble DLK-1 has been shown to stimulate angiogenesis (the formation of new blood vessels) by activating Notch1/Akt signaling in endothelial cells. In certain contexts, such as pancreatic cancer models, sDLK-1 exhibits antitumor effects by suppressing cell migration, wound healing, and colony formation. Because it is highly expressed in the fetus and placenta, maternal serum levels of sDLK-1 are used as a marker for fetal growth restriction and other pregnancy complications.
Tissue Specificity Expression in the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue.
Cellular Localization Secreted protein
Involvement In Disease It acts as a paracrine/endocrine regulator, with significant involvement in various diseases, particularly in bone metabolism, obesity, cancer, and cardiovascular fibrosis

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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