Recombinant Human Serine/Threonine-Protein Phosphatase 2B Catalytic Subunit Gamma Isoform (PPP3CC) Protein (His)

Name: Recombinant Human Serine/Threonine-Protein Phosphatase 2B Catalytic Subunit Gamma Isoform (PPP3CC) Protein (His)
Brand: Beta LifeScience
SKU: BLC-10038P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Serine/Threonine-Protein Phosphatase 2B Catalytic Subunit Gamma Isoform (PPP3CC) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P48454
Target Symbol	PPP3CC
Synonyms	calcineurin A gamma; CALCINEURIN A3; Calcineurin; Calcineurin testis specific catalytic subunit; Calmodulin dependent calcineurin A subunit gamma isoform; Calmodulin-dependent calcineurin A subunit gamma isoform; CALNA3 ; CAM PRP catalytic subunit; CAM-PRP catalytic subunit; CNA3 ; pp2b gamma; PP2BC_HUMAN; PP2Bgamma; PPP3CC; protein phosphatase 3 (formerly 2B) catalytic subunit gamma isoform (calcineurin A gamma) ; protein phosphatase 3 (formerly 2B) catalytic subunit gamma isoform; Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform; testis-specific catalytic subunit
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	SGRRFHLSTTDRVIKAVPFPPTQRLTFKEVFENGKPKVDVLKNHLVKEGRLEEEVALKIINDGAAILRQEKTMIEVDAPITVCGDIHGQFFDLMKLFEVGGSPSNTRYLFLGDYVDRGYFSIECVLYLWSLKINHPKTLFLLRGNHECRHLTDYFTFKQECRIKYSEQVYDACMETFDCLPLAALLNQQFLCVHGGMSPEITSLDDIRKLDRFTEPPAFGPVCDLLWSDPSEDYGNEKTLEHYTHNTVRGCSYFYSYPAVCEFLQNNNLLSIIRAHEAQDAGYRMYRKSQATGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELISDDEAEGSTTVRKEIIRNKIRAIGKMARVFSILRQESESVLTLKGLTPTGTLPLGVLSGGKQTIETATVEAVEAREAIRGFSLQHKIRSFEEARGLDRINERMPPRKDSIHAGGPMKSVTSAHSHAAHRSDQGKKAHS
Expression Range	2-512aa
Protein Length	Partial
Mol. Weight	62.0kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. Dephosphorylates and activates transcription factor NFATC1. Dephosphorylates and inactivates transcription factor ELK1. Dephosphorylates DARPP32.
Protein Families	PPP phosphatase family, PP-2B subfamily
Database References	HGNC: 9316 OMIM: 114107 KEGG: hsa:5533 STRING: 9606.ENSP00000240139 UniGene: Hs.731683
Tissue Specificity	Testis.

Gene Functions References

PPP3CC rs7431 may alter miRNA binding ability of miR-212 and miR-132, and thus decrease bladder cancer risk. PMID: 29275364
These findings indicated that the down-regulation of PPP3CC by ZEB1 resulted in activation of NF-kappaB is a critical oncogenic event in gliomas. PMID: 29294030
The rs2443504 AA genotype was associated with the Glasgow coma score at 3, 6, and 12 months following severe brain injury. PMID: 27225880
Knockdown of CNAgamma leads to a disruption of synaptic vesicle cycling in hippocampal neurons. PMID: 26627835
The PPP3CC involved in the regulation of immune system and synaptic plasticity, seems promising for further investigation. PMID: 24709691
protein level of PPP3CC negatively correlates with the cellular level of IP3, suggesting a regulatory role of PPP3CC in the IP3-Ca2+ signaling pathway PMID: 23747857
study suggests that NMDA receptor-mediated signalling genes, DAO, PPP3CC, DTNBP1 might be involved in schizophrenia pathogenic mechanisms related to gender PMID: 23497497
This study demoistrated that PPP3CC are not genetic risk factors for schizophrenia in japanese. PMID: 20537399
results identify PPP3CC, located at 8p21.3, as a potential schizophrenia susceptibility gene and support the proposal that alterations in calcineurin signaling contribute to schizophrenia pathogenesis PMID: 12851458
Decreased hippocampal expression of the susceptibility gene PPP3CC in schizophrenia. PMID: 15820226
PPP3CC may not play a major role in Japanese schizophrenia PMID: 15843870
The results suggest that PPP3CC gene may be a true susceptibility gene for schizophrenia. PMID: 17339875
The Polymorphism, Single Nucleotide of ppp3cc is assosciated with schizophrenia. PMID: 17895921
No significant differences in calcineurin A gamma expression was observed between patients with schizophrenia and normal controls PMID: 18343007
Data suggest that PPP3CC does not elevate the risk of methamphetamine-use disorder in the Japanese population. PMID: 18991849

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.